We narrowed to 745 results for: nes

1. The Importance of a Fun Workplace: Company Culture at Addgene

   Type

   Blog Post

   Published

   Sept. 3, 2015, 2:30 p.m.

   ...place to work, we do SO many fun things!!”  Okay - honestly, maybe not everyone replies with quite so much...

2. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

   Type

   Blog Post

   Published

   Sept. 23, 2014, 3:05 p.m.

   ...you like to bring your phone in the lab, though, honestly, this still grosses me out. Maybe it’s just me...

3. Hot Plasmids - August 2020

   Type

   Blog Post

   Published

   Aug. 18, 2020, 1:15 p.m.

   ...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

4. Plasmids 101: Plasmid Incompatibility

   Type

   Blog Post

   Published

   March 31, 2020, 1:15 p.m.

   ...pathogenic Ti plasmids in Agrobacterium tumefaciens. Genes & Genetic Systems 77:1–9 . https://doi.org/10.1266...

5. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...temperature than its Spinach ancestors, and has a lower magnesium dependence. Though initially still unstable in...

6. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   Published

   July 30, 2024, 1:15 p.m.

   ...piece of the viral vectors service puzzle: the usefulness of each viral prep in practice. The number of...

7. Antibodies 101: Immunoprecipitation

   Type

   Blog Post

   Published

   Dec. 7, 2021, 2:15 p.m.

   ... Common negative controls include knockout cell lines or tissues that do not express the protein. With...

8. Hot Plasmids - November 2023

   Type

   Blog Post

   Published

   Nov. 14, 2023, 3:35 a.m.

   ...Through Envelope Design). This modular approach combines an interchangeable set of fusogenic and targeting...

9. Viral Production at Addgene

   Type

   Blog Post

   Published

   Oct. 24, 2023, 1:15 p.m.

   ... plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences...

10. How to Be an Excellent Trainee

    Type

    Blog Post

    Published

    Sept. 26, 2023, 1:15 p.m.

    ...mindful that your mentor will still have their own deadlines and priorities to attend to. Being mindful looks...

11. Changing Labor Laws Bring Increased Postdoc Wages

    Type

    Blog Post

    Published

    Oct. 25, 2016, 2:30 p.m.

    ...Yourself for a New Career in Science with Our Career Readiness Posts Learn Good Management for Scientists Resources...

12. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    Published

    Oct. 20, 2016, 2:30 p.m.

    ...You can check out the r/Science AMA Submission Guidelines here, but essentially we just needed to send ...

13. Important Considerations in Optogenetics Behavioral Experiments

    Type

    Blog Post

    Published

    Oct. 1, 2015, 2:30 p.m.

    ...Witten, Ilana B., et al. "Recombinase-driver rat lines: tools, techniques, and optogenetic application ...

14. Plasmids 101: E. coli Strains for Protein Expression

    Type

    Blog Post

    Published

    Feb. 10, 2015, 3:06 p.m.

    ...below or browse Addgene’s collection of empty backbones.   Our Most Popular Plasmids 101 Posts: Origin...

15. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    ...make stricter gates while some like to make looser ones (Figure 3). A more “liberal” gate in the beginning...

16. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ...must be recoded to ‘TGA’ using site-directed mutagenesis. In the C321.ΔA strain, this TGA will be recognized...

17. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    Published

    Nov. 25, 2015, 4 p.m.

    ... At Addgene we try to do our part in boosting awareness of various career opportunities through our career...

18. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ...Brenner, M. (2004). Expression specificity of GFAP transgenes. Neurochemical Research, 29(11 SPEC. ISS.), 2075...

19. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ...interpret. Check out (Jambor et al., 2021) for more guidelines for creating clear and informative figures. Pro-tip...

20. A Control for All Seasons

    Type

    Blog Post

    Published

    Oct. 5, 2023, 1:15 p.m.

    ...Western blotting When comparing samples in different lanes in a western blot, you’ll need to include a loading...