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Showing: 371 - 380 of 602 results

  1. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ..., their makeup (in terms of nationality, gender, race, etc), or their career outcomes. Some of the more...their mentorship with the same critical eye they place on others within their labs - as a sort of over-...

  2. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...expressed on T cells to make CAR T cells. The extracellular scFv portion of the receptors recognizes an ...an antigen of interest while the intracellular T cell receptor portion of the CAR facilitates signal transduction...

  3. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...structural complexity inside the cell or on its surface. Bear in mind, the voltage setting of the laser...through conjugated antibodies targeting the cell surface markers CD11 (anti-mouse CD11b-BV510), which is...

  5. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...but is activated by GAL4 binding to UAS. If you place the GAL4 gene downstream of a tissue- or developmental...inhibiting the binding of GAL4 to UAS. One can therefore place GAL4 and GAL80 under different promoters and create...

  6. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...and the ATP dependent 9°NTM DNA Ligase are not replacements for T4 DNA Ligase in applications such as routine...PMCID: PMC306403. 11. Wu, Dan Y., and R. Bruce Wallace. "Specificity of the nick-closing activity of bacteriophage...

  7. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...function. After analysis, CRISPR classifications were placed into a fairly simple hierarchy: class to type to.... However, knowing the basic diversity and the surface-level function of each type could lead you to new...

  8. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ... presence or absence of certain markers on the surface or the inside of your cells. While proteins themselves...plasma membrane and can be detected through a cell surface label. The viral vector also encodes for eGFP, ...

  10. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...multiplex your reference genes to save on plate space. For many cell lines and tissue types, appropriate...your reference gene set can save significant plate space and may be worth the initial effort of setting it...
Showing: 371 - 380 of 602 results