We narrowed to 803 results for: TIM

1. Improved Plasmid Maps Powered by SnapGene

   Type

   Blog Post

   Published

   June 29, 2017, 1:06 p.m.

   ...will cut (in basepairs), and the total number of times they cut. Use the filters on the right to find single...

2. Plasmids 101: Yeast Vectors

   Type

   Blog Post

   Published

   Feb. 25, 2014, 7:11 p.m.

   ...replication of these vectors. S. pombe plasmids oftentimes utilize an ARS to aid in high transformation ...

3. Antibodies 101: Fab Fragments

   Type

   Blog Post

   Published

   Sept. 5, 2024, 1:15 p.m.

   ...affinity reagents, and for good reason. However, sometimes you want a tool that is a little more compact ...

4. Antibodies 101: Conjugation

   Type

   Blog Post

   Published

   March 26, 2024, 1:15 p.m.

   ...antibodies are provided in a buffer with the antimicrobial agent sodium azide, which can interfere with...

5. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   Published

   March 19, 2019, 1:31 p.m.

   ...inserting electrodes into the brain. But this process is time consuming and expensive. Thus scientists have turned...

6. Transferable Skills Guide: Cross-team Communication

   Type

   Blog Post

   Published

   Nov. 21, 2019, 1:53 p.m.

   ... protocols, SOPs, and methods sections. The next time you write or read one of these, keep an eye out ...

7. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

   Type

   Blog Post

   Published

   Oct. 29, 2019, 2:52 p.m.

   ... light (590nm). BLINK2 potassium channel - An optimized blue-light induced synthetic potassium channel...

8. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   Published

   Dec. 16, 2015, 3:30 p.m.

   ...cumbersome, can lower on-target efficiency, and sometimes even increase off-target effects, respectively...

9. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   Published

   Oct. 20, 2015, 2:30 p.m.

   ... and students will again gather in Boston - this time with the aim of finding solutions to some of the...

10. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...desired edit to still occur if the cut site is sub-optimal? As with everything in biology, the answer is always...

11. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ... The Junwei Shi Lab addressed this problem by optimizing the Cas protein delivery with Peptide-Assisted...

12. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ...treat these diseases (drug repositioning). The ultimate goal of this work is to lower the burden of immune...

13. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ... for viewing how gene expression changes through time or after genetic manipulation. The technique involves...

14. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ... interact with the bait at a particular point in time, i.e. when the bait is interacting with a particular...

15. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...in vivo studies. There are different versions optimized for neuron-specific, astrocyte-specific (GCaMP6...

16. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...1. In BSL-2 labs, eye protection is required any time you have to work with BSL-2 materials, such as lentivirus...

17. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ...greater amount of antigen in a shorter period of time and would likely perform better for probing poorly...

18. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...genome of an hiPS cell. While the majority of the time the genome will attempt to repair itself without...

19. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...the MTA. Pro Tip! Download a new spreadsheet each time you want to submit new plasmids! We update the spreadsheet...

20. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...this TIR1 complex binds to the degron sequence ultimately leading to the degradation of the AID-tagged ...