We narrowed to 800 results for: Des

1. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   Published

   Nov. 10, 2022, 2:15 p.m.

   ... another way, only cells or organisms with the desired phenotype will survive and be assayed at the end...

2. OCaMP: A New Calcium Indicator for Neural Imaging

   Type

   Blog Post

   Published

   Aug. 19, 2025, 1:15 p.m.

   ... from University of Calgary. Over the past few decades, genetically encoded calcium indicators (GECIs)...

3. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ...2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your gRNA. It’s the former version...

4. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ...focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with actionable steps...

5. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ... that you know how to read flow plots and have designed your first flow panel, you’ll load your samples...

6. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   Published

   Aug. 7, 2014, 1:58 p.m.

   ... don’t overdo it (some of my best studies were designed over late night poker games at Gordon Conferences...

7. 3 Challenges in Plant Synthetic Biology

   Type

   Blog Post

   Published

   July 22, 2014, 5:46 p.m.

   ...have been a model organism for plant research for decades, and the biochemical pathways involved in central...

8. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

   Type

   Blog Post

   Published

   Feb. 2, 2018, 3:20 p.m.

   ...these difficult pieces: “It’s probably the best.” Besides working through the tough assignments, the skills...

9. RNA Extraction Without A Kit

   Type

   Blog Post

   Published

   March 24, 2020, 1:15 p.m.

   ...Allewell and Sarma, 1974). Additionally, ThermoFisher provides a protocol on how to integrate RNAlater® with ...

10. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    Published

    Dec. 21, 2021, 2:15 p.m.

    ...affinity, many indirect antibody protocols are designed to take place over multiple days. Increased run...

11. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ...novel RNA-protein interactions, Ramanathan et al. designed an RNA bait containing three well conserved but...

12. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    Published

    Jan. 31, 2019, 1:32 p.m.

    ...to the development and spread of cancer cells. Besides these stromal cell modifications, stromal cells...

13. Antibodies 101: Fab Fragments

    Type

    Blog Post

    Published

    Sept. 5, 2024, 1:15 p.m.

    ...talking about here? Many of the Fab fragments described above can be used for similar applications and...

14. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    Published

    April 13, 2017, 2:30 p.m.

    ... phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally PHO8 ...

15. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    Published

    Jan. 11, 2017, 3:30 p.m.

    ...the postdoctoral population which keeps growing despite a lack of available academic positions.   Many...

16. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    Published

    May 5, 2015, 3:54 p.m.

    ...down your own results; are they the same as the description the authors give? Ask yourself if the results...

17. Working with Nuclear Receptors

    Type

    Blog Post

    Published

    July 15, 2014, 4:03 p.m.

    ...Activation of membrane receptors and signaling cascades conceivably allows the genome to sense the impact...

18. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ...specific protein-protein interactions, SplitBioID provides a candidate list of proteins that are part of ...

19. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources at Addgene.org Find...

20. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...that are built into how something functions, were designed for this purpose. One such control is dividing...