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Showing: 741 - 760 of 855 results

  1. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...academic labs. Being unable to publish or attend conferences makes it very challenging to compete in today...pressure to publish and more freedom to attend conferences, but after receiving her degree she was faced...

  2. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...against improper, random recombination into a different locus. Correctly recombined cells will not contain...should increase. New genome engineering tools also offer hope for the creation of new knockout animal models...

  3. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...species, like S. aureus and N. meningitidis require different PAM sites than SpCas9, in this case, NNGRRT and...is working for you in the comments section! References Hu, Johnny H., et al. "Evolved Cas9 variants ...

  5. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ... the process is similar to academia. The only difference is that your paper will be reviewed by an internal...LinkedIn connections and other contacts to get that referral and find out more about the company’s program....

  6. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making... the target DNA strand, and then activate two different endonuclease active sites. Fortunately, there ...

  7. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...mixed population, represented here by cells of different colors, specifically targets bacteria with the...ground in the fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark, and Timothy...

  8. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...expertise with our district middle school was a very different experience since, unlike elementary schools, middle...such a valuable extension to our curriculum and offered engaging examples of how understanding genetics...

  9. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...truth, people are incredibly varied in how they prefer to consume information. Personally, I would much...outcome to the limitations and potential of your preferred platform before you start can save a lot of trouble...

  10. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...human genome-wide CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do...conditions such as model systems and primary cells. References 1. Doench, John G., et al. "Rational design of...

  11. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...comes down to the researcher’s familiarity with different systems.  Using Cas9-gRNA RNPs in your experiments...include genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse...

  12. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...Joanne’s back yard this year, though she generously offered. Instead we picniced outside of our new office ...Netflix series. The thing that has made all the difference here at Addgene is the emphasis placed on team...

  13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...Red-shifts the wavelength (yellow derivatives) A206K Interferes with dimer interface (monomeric derivatives) ...earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions...

  15. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...existing comprehensive mutagenesis methods, PFunkel offers the best combination of library coverage, mutational...mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al. "Epistasis...

  16. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...similar properties to Broccoli despite binding to a different small molecule. Notably, like Broccoli, Red Broccoli...reports on the next additions to aptamer soup! References Alam, Khalid K., et al. "A fluorescent split ...

  17. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle Charpentier for their pioneering...more at his webpage or follow him on Twitter.   References Arribere JA, Bell RT, Fu BXH, Artiles KL, Hartman...

  18. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ...are, their role in the immune response, and the different antibody isotypes found in mammals.      Subscribe...

  19. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...guide. Print out the guide and use it for quick reference when you're designing your next virus experiment...

  20. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...support use of their antibody tools. Addgene also offers a growing collection of educational resources on...
Showing: 741 - 760 of 855 results