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We narrowed to 902 results for: Ide

Showing: 741 - 760 of 902 results

  1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...proteins to be imaged together, you will need to consider the same factors as when choosing an individual... the filter sets or lasers on your microscope. Ideally, you would like to have substantial overlap between...protein and a filter set, many filter set vendors provide tools to plot the fluorescence spectra of proteins...

  2. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... kB, then you would be oversized even before considering the need for some manner of polyA. In this scenario...can be safely concentrated to ~1x1014 vg/ml.. As evident in our repository, this application is an outlier...Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors Blog Posts ...

  4. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...promoter should flank this component as well. On both sides of this promoter-gene combo are two long terminal... and have appropriate factors displayed on the outside of the particle to interact with the host. This...applications and aid in ease of use. Our lentiviral guide outlines these components.     Fig. 1 - ...

  5. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...within a biological system. Basically, biosensors provide quantifiable readouts for levels of different chemicals...FRET-based biosensors have been engineered to detect a wide array of neurotransmitters ranging from dopamine...fluorescence-based and have been optimized for a wide-range of uses to monitor the fundamental cellular...

  6. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...based on maps of protein structure. Sometimes this provides a great deal of clarity. But, often these methods...Institute Target Accelerator Plasmid Collections, now widely available to the scientific community through Addgene...Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y....

  7. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...check out this blog post or see Addgene's CRISPR guide page for a review of Cas9.  For a brief comparison...Essletzbichler, P.,  Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell...Research Catch up on Your CRISPR Background with Our Guide Pages ...

  8. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...there are many great resources available to help guide you through the rough waters of mutation validation...great deal of precision. This means digital PCR provides an absolute measure of target abundance, does ...screening to validate your genome edits, this paper provides detailed instructions on how to design such assays...

  9. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ... and gotten unusual or unexpected results? Or considered why DpnI will degrade your template DNA from ...methylated (if it comes from a phage for instance). Aside from restriction modification systems, DNA methylation...level, the potential for methylation should be considered when digesting DNA. Why? Well, even though Dam...

  10. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...task and will prevent you from getting lost in the wide word of informatics. I am a molecular biologist ... project a few years ago when my advisor and I decided to do a massive RNA-seq analysis. I had never interacted...even if your conclusion is “this approach is not ideal because…” Including a conclusion for each analysis...

  11. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...can be reconstituted in vivo to form a protein identical in sequence to the unmodified enzyme. Each half...any exogenous sequences and regenerates a native peptide bond at the split site. Figure 1: The base...

  12. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...interactome of these key regulatory molecules, and has provided clues to their previously uncharacterized cellular...acetyltransferase to luciferase, the basic assay remains widely used today.  The sheer abundance of these critical...greatly increased the burden associated with identifying plasmids in the research literature and acquiring...

  13. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...specificity of AAV, work has focused on modifying the outside protein shell, or capsid, of the virus so that ...here.  Applications for synthetic promoter AAVs Besides restricting AAV transgene expression to particular...synthetic promoters with high rates of labeling are ideal of gene therapy applications, promoters that produce...

  14. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...molecular tools to map neuronal connectivity are widely used in neuroscience. In this post, I’ll describe... in first order neurons, the G-deleted RABV can divide and replicate (since these processes are mediated...Another study used RABV monosynaptic tracing to identify inputs to two major dopaminergic cell populations...

  15. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...initiation step of gene transcription; today we'll provide an overview on how transcription stops, or termination...post-transcriptional additional of multiple adenine (A) nucleotides to the tail of a messenger RNA transcript. The...be detailed here, but additional references are provided at the end.  Nearly all common bacterial expression...

  16. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide off-targets, since increasing... Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn...

  17. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...below. Types of Voltage Indicators GEVIs can be divided up structurally into 2 main groups: voltage sensitive...voltage sensing component (i.e., rhodopsin naturally resides in the membrane), or both. Targeting these systems...Addgene’s Virus Protocols Addgene’s Chemogenetics Guide Resources on the Addgene blog Viral Vectors ...

  18. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...Even before fluorescent proteins (FPs) came into wide use, there were a variety of ways to monitor cell...few properties that have now become hallmarks of ideal FP timers: 1. Ratiometric determination of expression...Empty Backbones Check Out Our Fluorescent Protein Guide ...

  19. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...that popularity through the years.  Libraries are divided into different categories based on function: knockout... years, even as more CRISPR technologies become widely used and available. If we look closely at Figure...navigate that learning curve. As of 2023, our CRISPR guide has been viewed 1,904,462 times, and the CRISPR ...

  20. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...Degrons are destabilizing domains which will be identified by various enzymes and sent to the proteasome...protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC...some stage, then choosing a HaloTag could pay dividends down the road. It is difficult to predict precisely...
Showing: 741 - 760 of 902 results