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We narrowed to 895 results for: Ide

Showing: 741 - 760 of 895 results

  1. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...within a biological system. Basically, biosensors provide quantifiable readouts for levels of different chemicals...FRET-based biosensors have been engineered to detect a wide array of neurotransmitters ranging from dopamine...fluorescence-based and have been optimized for a wide-range of uses to monitor the fundamental cellular...

  2. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ... and gotten unusual or unexpected results? Or considered why DpnI will degrade your template DNA from ...methylated (if it comes from a phage for instance). Aside from restriction modification systems, DNA methylation...level, the potential for methylation should be considered when digesting DNA. Why? Well, even though Dam...

  3. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...task and will prevent you from getting lost in the wide word of informatics. I am a molecular biologist ... project a few years ago when my advisor and I decided to do a massive RNA-seq analysis. I had never interacted...even if your conclusion is “this approach is not ideal because…” Including a conclusion for each analysis...

  4. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...can be reconstituted in vivo to form a protein identical in sequence to the unmodified enzyme. Each half...any exogenous sequences and regenerates a native peptide bond at the split site. Figure 1: The base...

  5. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...interactome of these key regulatory molecules, and has provided clues to their previously uncharacterized cellular...acetyltransferase to luciferase, the basic assay remains widely used today.  The sheer abundance of these critical...greatly increased the burden associated with identifying plasmids in the research literature and acquiring...

  6. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...molecular tools to map neuronal connectivity are widely used in neuroscience. In this post, I’ll describe... in first order neurons, the G-deleted RABV can divide and replicate (since these processes are mediated...Another study used RABV monosynaptic tracing to identify inputs to two major dopaminergic cell populations...

  7. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...specificity of AAV, work has focused on modifying the outside protein shell, or capsid, of the virus so that ...here.  Applications for synthetic promoter AAVs Besides restricting AAV transgene expression to particular...synthetic promoters with high rates of labeling are ideal of gene therapy applications, promoters that produce...

  8. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...initiation step of gene transcription; today we'll provide an overview on how transcription stops, or termination...post-transcriptional additional of multiple adenine (A) nucleotides to the tail of a messenger RNA transcript. The...be detailed here, but additional references are provided at the end.  Nearly all common bacterial expression...

  9. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide off-targets, since increasing... Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn...

  10. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...Even before fluorescent proteins (FPs) came into wide use, there were a variety of ways to monitor cell...few properties that have now become hallmarks of ideal FP timers: 1. Ratiometric determination of expression...Empty Backbones Check Out Our Fluorescent Protein Guide ...

  11. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...that popularity through the years.  Libraries are divided into different categories based on function: knockout... years, even as more CRISPR technologies become widely used and available. If we look closely at Figure...navigate that learning curve. As of 2023, our CRISPR guide has been viewed 1,904,462 times, and the CRISPR ...

  12. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...Degrons are destabilizing domains which will be identified by various enzymes and sent to the proteasome...protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC...some stage, then choosing a HaloTag could pay dividends down the road. It is difficult to predict precisely...

  13. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...below. Types of Voltage Indicators GEVIs can be divided up structurally into 2 main groups: voltage sensitive...voltage sensing component (i.e., rhodopsin naturally resides in the membrane), or both. Targeting these systems...Addgene’s Virus Protocols Addgene’s Chemogenetics Guide Resources on the Addgene blog Viral Vectors ...

  15. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...factor of 2. This means that if you set up two identical assays with the same sample, you could end up ... should fall within the range of the assay for a wide range of titers. We usually dilute our samples 1...experiments.  References Furuta-Hanawa, Birei, Teruhide Yamaguchi, and Eriko Uchida. "2D droplet digital...

  16. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... codon, stop codons, reading frame considerations, etc). This video demonstrates how to use the Snapgene...Protocols Watch Our Diagnostic Restriction Digest Video Check Out Our Molecular Biology Reference Pages  ...

  17. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...sites. FRT sites and loxP sites differ at the nucleotide level but share an overall structure of 13 base...37 °C). Smart molecular evolution led to the identification of FLPe which has a temperature optimum of ...Recombination between the two target sites with identical sequence leads to inversion of the flanked DNA...

  18. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...more funding and research opportunities are being provided to immunotherapy than ever before. Although different...from many other types of cancer. Mesothelioma is considered an orphan cancer; it is rare disease with only...(ADAO) are working toward such a ban, and the president of the organization, Linda Reinstein – whose husband...

  19. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...fluorescence can occur. Cre recombinase can be used alongside common, existing tools for fly research that use...turnover of Langerhans cells in the dermis and identify that Langerhans cells are replenished through ...The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations." Proceedings...

  20. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...results in a polymerase error rate of up to 2% per nucleotide position. The “sloppy” PCR reaction is created...ssDNA is packaged into phage and then extracted. Besides taking a couple days to generate the phage-derived...strand: A mix of degenerate primers and the high-fidelity Phusion polymerase are used to introduce point...
Showing: 741 - 760 of 895 results