We narrowed to 804 results for: arc
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TypeBlog PostPublishedOct. 1, 2019, 1:21 p.m.... culture and connections. Start your interview research there! 6) Your LinkedIn profile should be as good...
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Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....Researchers express genes of interest from plasmids in order to study gene function or to engineer cells... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....photoswitchable kinases in vivo with C. elegans. Researchers commonly use rectal bacterial infection to induce... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog PostPublishedApril 13, 2017, 2:30 p.m....Levine, B. (2010). Methods in Mammalian Autophagy Research. Cell,140(3), 313-326. doi:10.1016/j.cell.2010.01.028... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....protocols! We invite feedback from all members of the research community to help us develop these resources further... -
The time and cost required to make a plasmid
TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m....special collection pages to more easily find plasmids Search for a plasmid in Addgene’s repository ... -
To Each HIS Own
TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m....Much of today's biological research requires a close examination of specific proteins within a system... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi:10.1093/nar/gku1198... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog PostPublishedAug. 1, 2024, 1:15 p.m....reprobe your western blot several times — some researchers report up to ten cycles! Just remember that a... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....fluorescence-activated cell sorting (FACS) helps researchers sift through thousands of cells to identify cells... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....p300 or the DNA methyltransferase DNMT3A allows researchers to easily add biochemical marks to genetic regions... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m.... viral protocols and videos Find AAVs for your research ... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 1, 2018, 1:57 p.m....half-lives. Interested in using Split-BioID with your research? Plasmids are available from Addgene! References... -
Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m....After all, there’s no sense in spending valuable research dollars on a new secondary antibody if you don... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m.... Before constructing your own FRET probe, try searching PubMed for articles describing the FRET tool that... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....alleles, and the growing need for gene editing in research, the old PAM constraints just won’t cut it anymore... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....expression in a context-dependent manner. Nucleic Acids Research. 2000. PubMed PMID:10648781. PubMed Central PMCID... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....resistance-genes, or the transgene itself. Many researchers prefer to design primers that target the common... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog PostPublishedJan. 21, 2015, 1:30 p.m.... to Expedite Your Experiments and Achieve Your Research Goals ... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....using adenoviral vectors with your CRISPR/Cas9 research! To find more information about the adenoviral...