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... plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences...

...mindful that your mentor will still have their own deadlines and priorities to attend to. Being mindful looks...

...Yourself for a New Career in Science with Our Career Readiness Posts Learn Good Management for Scientists Resources...

...You can check out the r/Science AMA Submission Guidelines here, but essentially we just needed to send ...

...Witten, Ilana B., et al. "Recombinase-driver rat lines: tools, techniques, and optogenetic application ...

...below or browse Addgene’s collection of empty backbones.   Our Most Popular Plasmids 101 Posts: Origin...

...make stricter gates while some like to make looser ones (Figure 3). A more “liberal” gate in the beginning...

...must be recoded to ‘TGA’ using site-directed mutagenesis. In the C321.ΔA strain, this TGA will be recognized...

... At Addgene we try to do our part in boosting awareness of various career opportunities through our career...

...Brenner, M. (2004). Expression specificity of GFAP transgenes. Neurochemical Research, 29(11 SPEC. ISS.), 2075...

...interpret. Check out (Jambor et al., 2021) for more guidelines for creating clear and informative figures. Pro-tip...

...Western blotting When comparing samples in different lanes in a western blot, you’ll need to include a loading...

... comb, run the gel on the slower side, and skip lanes between samples. In addition to a DNA ladder standard...

...of these decisions is complex, here are a few guidelines to help you evaluate projects:   If all of your...

...constructs have facilitated the creation of mouse lines that can be used in fate mapping experiments. Immune...

...plasmid purification is always an option, let’s be honest — kits are quick, easy, and don’t involve phenol-chloroform...

...article). The group first tested photocaging various lysines in Cas9 to determine which best deactivated the...