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We narrowed to 874 results for: sta

Showing: 741 - 760 of 874 results

  1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ... into the bacterial genome. REPLACR-mutagenesis starts out in familiar territory, but quickly diverges...recombination then joins the linear PCR products into stable circular plasmids - no nick repair is needed. Since...

  2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...only 44% and 66% indel frequency with the use of standard single gRNA constructs. When PTG constructs with...<200 bp tRNA-gRNA takes up half the space of a standard pol III-gRNA, which will facilitate CRISPR/Cas9...

  3. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...intervening DNA (“pop-out” deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to...References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM (2009) Limiting the Persistence of a Chromosome...

  4. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...several considerations. First, it is important to establish the type of audience you want to reach with your...and help you correct any language- and spelling mistakes. A manuscript that is well written looks more ...

  5. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...DNA-protein interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect ...interactions. To address this need, the Khavari lab at Stanford created the RNA-protein interaction detection ...

  7. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...resulting in GFP signal. With the addition of standards, the assay is semi-quantitative and can approximate...plasmids and suitability of various antibiotic resistance markers, promoters, and inducible gene expression...

  8. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...possible antibody conjugates (especially if you start to consider clinical applications). But there are...make the process quick and easy. Before getting started, be sure to check that your antibody’s buffer is...

  9. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...However, transcription of thousands of genes is constantly occurring throughout a single cell, so these ... these structures can in turn lead to genome instability. Thus, it’s important to strike a balance between...

  11. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...use electroporation, a technique that briefly destabilizes the cell’s outer membrane and allows the components...structures and processes and can lead to the better understanding of cell biology. We hope that these high-quality...

  12. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...vivo studies should always take DNA’s chromatin status into account (Fig 1a). The laboratory of Karmella...dCas9 expression and contain different antibiotic resistance markers. New CRISPR pooled library from Keith...

  14. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...that treat disease at the molecular level. For instance, if a disease is caused by a mutation in a single...developing AAV-based therapies. Furthermore, deeper understanding of disease pathogenesis will enable robust therapeutic...

  15. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...considered "Cre-On" meaning that your gene of interest starts in the inverse/antisense "off" position and is ...employed for "Cre-off" (i.e. your gene of interest starts in the sense orientation and Cre is used to flip...

  16. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...light up endogenous genes of interest to better understand normal protein function. However, CRISPR protein...introduced directly into a Drosophila S2 cell line stably expressing Cas9. This PCR toolkit offers C- and...

  17. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...et al., 2024) have highlighted how chromatin substantially affects prime editing outcomes across many genomic...with PRIDICT/PRIDICT2.0, ePRIDICT can help you understand how chromatin might influence editing results...

  18. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...is carefully designed primers. The majority of mistakes are due to incorrect primer sequences! Primers...transduction, and uses electron cryo-microscopy to understand receptor function.     Follow them on Twitter...

  19. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...CNS and PNS! What is the CREATE method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution...number of neurons overall (GFP+ cells that also stained for a neuronal marker). Check out tables 1 and ...

  20. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...randomly, or at fixed genomic target sites.  This obstacle was recently surpassed with the discovery of CRISPR-transposons...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...
Showing: 741 - 760 of 874 results