We narrowed to 803 results for: TIM
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TypeBlog PostPublishedAug. 10, 2023, 1 p.m....in vivo studies. There are different versions optimized for neuron-specific, astrocyte-specific (GCaMP6...
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Viral Vectors 101: Virus Safety
TypeBlog PostPublishedJune 22, 2023, 1:15 p.m....1. In BSL-2 labs, eye protection is required any time you have to work with BSL-2 materials, such as lentivirus... -
Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m....greater amount of antigen in a shorter period of time and would likely perform better for probing poorly... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....desired edit to still occur if the cut site is sub-optimal? As with everything in biology, the answer is always... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m.... The Junwei Shi Lab addressed this problem by optimizing the Cas protein delivery with Peptide-Assisted... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m....allowing for rescued expression of GeneR. Sometimes also referred to as DiO (Double-floxed inverse... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation is the post-... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....so far! Why do we need PAM flexibility? IDT estimates that there is an NGG PAM every 42 bases in the... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can be exploited to... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....of course, only hypothetical at this point. More time and energy must be devoted to studying Cas3 and ... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m....expressed in non-dividing cells for long periods of time. This reduces the chance of integration at random... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....before moving onto your experimental samples. With time and practice, you may be able to do your compensation... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m.... Editing by John Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....available through Addgene, with more being added all the time. Keep in mind that libraries are only as good as... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m....appropriate immunizing reversal drive at the same time to mitigate the potential effects of an accidental... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....directed evolution across the full ADAR2DD to further optimize cytidine deaminase activity. While RESCUE can ... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....antibodies from its serum, and meticulously screening, optimizing, and characterizing several anti-mouse and -rabbit... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....activate the immune system, and of the infection to sometimes result in cell death, has led to interest in using... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....ancestry group. With the existing methodology, they estimate that the African ancestry-associated cancer dependencies... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....titration experiments might be helpful to achieve optimal results. It is important to note that this system...