We narrowed to 889 results for: ANT;
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TypeBlog PostPublishedJune 10, 2025, 1:15 p.m....loss-of-function mutants could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward...
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An Addgene Summer Internship
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m....Scholars summer program. I was unsure whether or not I wanted to major in biochemistry. There, I had exchanged...has changed my life for the better, and I wouldn’t want it any other way. Toj Toj-Villagran is a rising ... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog PostPublishedSept. 12, 2023, 1:15 p.m....reagents. mAb3D atlas validated antibodies: A database of validated antibodies for mouse, human, and marmoset... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....researchers to select and generate physiologically relevant models. In complex diseases, it can aid in generation... line knock-ins of patient mutations which are variants of unknown significance – mutations with unclear... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....effector gene. Effector genes, any gene that you want to control the expression of using the SPARC system...length of the truncated attP site. The three SPARC variants, based on the length of the attP site, allow for... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog PostPublishedNov. 17, 2015, 3:30 p.m....chemistry degrees and provide advice to students who want to study chemistry. The society currently has 352...engaging with young scholars. We believe it’s very important to develop a network of fellow scientists that... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog PostPublishedJuly 2, 2015, 8:37 p.m....simply put, I moved into this position because I want to help make the research enterprise work better...at Harvard, I recognized that I would ultimately want to go into science communication but thought that... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog PostPublishedSept. 26, 2017, 12:12 p.m.... and data with authors and referees. And I am constantly teaching – often explaining a technical piece...focuses at Science on developmental neurobiology and plant sciences, viewed through the lens of development... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....were known to be diversity hotspots containing abundant biological dark matter and so the lab organized...application from the publication is the removal of unwanted cDNA derived from 16S rRNA in bacterial RNA-Seq... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly! The question The first, and most important question, you should ask for any science communication... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....that produce useful natural materials, such as antibiotics. Simon Moore and Paul Freemont’s lab previously...mCherry and GFP expression to check for ABE’s. b) Quantification of base-editing efficiency using XMAS-TREE-... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....as a drug-screening platform to identify new antifungals that target calcineurin. Current calcineurin ...consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition to showing... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m.... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward. Xeroderma... with CRISPR-Cas9. There are so many different variants and strategies out there. This will help us acquire... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m.... transduction? Niels Geijsen’s group in Utrecht wanted to pursue a nonviral strategy for transducing primary...indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating that... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....to publication and acquisition of funding. If a grant committee is not convinced that your project is ...getting a much-needed break from your lab bench. Want further information about shaping your career to... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog PostPublishedApril 18, 2019, 12:53 p.m.... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized...low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly, give CRISPR-... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m....while still creating scarless plasmid inserts. Want to Learn More about DNA Assembly? Check Out Our ...polymerase used in the amplification. This tool allows instant updates as fragments are added, deleted, or reordered... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog PostPublishedAug. 2, 2022, 1:15 p.m....bacteria are permeable to different classes of antibiotics. The classification system is also useful in ...inserted into a specific bacterium, it can be important to consider the type of bacteria it is being used... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog PostPublishedMarch 30, 2023, 1:15 p.m....Zhang lab’s previously published protocol and its relevant modifications on the pooled library webpage. Disease... that any researcher could use to generate the relevant disease model. Accurate modeling of a disease ...