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Showing: 761 - 780 of 889 results
  1. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...Scholars summer program. I was unsure whether or not I wanted to major in biochemistry. There, I had exchanged...has changed my life for the better, and I wouldn’t want it any other way. Toj Toj-Villagran is a rising ...
  2. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...researchers to select and generate physiologically relevant models. In complex diseases, it can aid in generation... line knock-ins of patient mutations which are variants of unknown significance – mutations with unclear...
  3. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...effector gene. Effector genes, any gene that you want to control the expression of using the SPARC system...length of the truncated attP site. The three SPARC variants, based on the length of the attP site, allow for...
  4. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...chemistry degrees and provide advice to students who want to study chemistry. The society currently has 352...engaging with young scholars. We believe it’s very important to develop a network of fellow scientists that...
  5. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ... and data with authors and referees. And I am constantly teaching – often explaining a technical piece...focuses at Science on developmental neurobiology and plant sciences, viewed through the lens of development...
  6. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly!  The question   The first, and most important question, you should ask for any science communication...
  7. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...that produce useful natural materials, such as antibiotics. Simon Moore and Paul Freemont’s lab previously...mCherry and GFP expression to check for ABE’s. b) Quantification of base-editing efficiency using XMAS-TREE-...
  8. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...
  9. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward. Xeroderma... with CRISPR-Cas9. There are so many different variants and strategies out there. This will help us acquire...
  10. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... transduction? Niels Geijsen’s group in Utrecht wanted to pursue a nonviral strategy for transducing primary...indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating that...
  11. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...to publication and acquisition of funding. If a grant committee is not convinced that your project is ...getting a much-needed break from your lab bench.  Want further information about shaping your career to...
  12. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized...low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly, give CRISPR-...
  13. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...while still creating scarless plasmid inserts.  Want to Learn More about DNA Assembly? Check Out Our ...polymerase used in the amplification. This tool allows instant updates as fragments are added, deleted, or reordered...
  14. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...bacteria are permeable to different classes of antibiotics. The classification system is also useful in ...inserted into a specific bacterium, it can be important to consider the type of bacteria it is being used...
  15. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...Zhang lab’s previously published protocol and its relevant modifications on the pooled library webpage. Disease... that any researcher could use to generate the relevant disease model. Accurate modeling of a disease ...
Showing: 761 - 780 of 889 results