We narrowed to 798 results for: Arc

1. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ...with either blasticidin or puromycin selection. Researchers at the Allen Institute for Cell Science recently...

2. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   Published

   Dec. 10, 2020, 1 p.m.

   ...using adenoviral vectors with your CRISPR/Cas9 research! To find more information about the adenoviral...

3. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

   Type

   Blog Post

   Published

   May 22, 2015, 4:58 p.m.

   ...exclusively because historical studies of pathogen research have conclusively shown that barrier protocols...

4. DNA Purification Without a Kit

   Type

   Blog Post

   Published

   Feb. 11, 2020, 2:15 p.m.

   ...number of spin columns you need to complete your research. Do you know of other nucleic acid purification...

5. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   Published

   Feb. 6, 2020, 3 p.m.

   ... of learning about new cutting edge science, researchers of all varieties must stay current with literature...

6. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   Published

   Feb. 27, 2018, 2:04 p.m.

   ...you ready to start using nanobodies for your own research? Great! Pleiner et al. have done the hard work...

7. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   Published

   Aug. 13, 2024, 1:15 p.m.

   .... Recombinant Antibodies in Basic Neuroscience Research. Curr Protoc Neurosci. 2020 Dec;94(1):e106. doi...

8. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

   Type

   Blog Post

   Published

   Aug. 30, 2022, 1:15 p.m.

   ...your target epitope(s). To achieve these goals, researchers commonly use one of two types of fixatives - ...

9. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...universally compatible with all bacteria used in research, it isn’t a widely popular selection agent. Pros...

10. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ...that retain their individual activities (1). Researchers also discovered the modular format of some transcriptional...

11. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...location and GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function,...

12. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...decades of trying to address these challenges, researchers have amassed a considerable molecular tool box...

13. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ...Resources on Addgene.org Find plasmids for your research Deposit plasmids at Addgene  ...

14. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    Published

    Jan. 3, 2019, 1:35 p.m.

    ... is an Ag-biotechnologist in the National Rice Research Institute, Cuttack, Odisha, India. Currently Kutubuddin...

15. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ... efficiency than its wild-type counterpart.  In search of a smaller, but equally potent Cas9 protein, ...

16. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ...Durbin AF, Kellner MJ, Tan AL, Paul LM, Parham LA, Garcia KF, Barnes KG, Chak B, Mondini A, Nogueira ML, ...

17. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    Published

    March 31, 2020, 1:15 p.m.

    ...et al., 2015). Now, bioinformatics tools help researchers determine how compatible their plasmids will ...

18. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    Published

    June 20, 2024, 1:15 p.m.

    ...initiation of chromosome replication. Nucleic Acids Research, 46(12), 6140–6151. https://doi.org/10.1093/nar...

19. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...genes that fell into 3 categories based on the architecture of the CRISPR protein in question: C2c1, C2c2...

20. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ...or as fluorophores in several different ways. Researchers have used oligonucleotides in fluorescent visualization...