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...AAV we have released in the last few months. You can also browse our entire AAV inventory. Our new AAVs...in target cells. While the various AAV serotypes can be used to achieve different patterns of expression...

... themselves from undue liability. Before Addgene can begin distributing a material, a deposit agreement...deposits with and/or requests materials from Addgene can qualify. We also reward improvement against one’s...Dana-Farber Cancer Institute  Fred Hutchinson Cancer Research Center Memorial Sloan-Kettering Cancer Center...

...research, they are now seen as an ubiquitous tool that can be diversely applied to many different experiments...Ed.) eBook And now for some exciting news... You can now download the newest version of Addgene's Plasmids...

...to engage in extracurricular activities (EA). EAs can: Introduce new perspectives in science and engineering...the big picture of the project. For example, how can future regulations impact your work? What design ...

... stats and more on the addgene.org homepage! You can track the number of plasmids right below the search...

...shared on our @addgene Instagram account, or you can watch the full animation on our YouTube channel.  ...

...cases, the selected mutation must be removed, which can be done by isolating animals with particular visible...become even more powerful following a report that one can use linear repair templates (ie. PCR-derived dsDNA...precise knock-ins, and homozygous knock-in animals can be obtained in eight days. The only animals on a ...2017). Because C. elegans has an AT-rich genome, it can be difficult to find an appropriate NGG PAM site ...repair of a temperature-sensitive pha-1 mutation significantly enriches for knock-in of 2x and 3xFLAG epitopes...

...itself a versatile option for tagging proteins, as it can be fused to the N- or C-terminus (or even in the ...., 2024 under CC BY license. This construct can be easily expressed and purified from E. coli, retains...light source, they have lower background signal and can be used in light-sensitive systems. Most excitingly...2024 American Chemical Society. Both PILATeS (18 kDa) and STReTCh (11 kDa) are significantly smaller... revealing antigenic differences between human, canine and simian isolates. J Gen Virol., 68 (Pt 11), ...expressing it with additional cofactors or using significantly truncated forms that limit its functionality...

...general guide to the effect on higher education, you can see that those in teaching roles (such as adjuncts...communications with and research on these institutions, we can say with relative certainty that just over 40% of...at some institutions; and that institutions which can more easily provide bridge funding to absorb the ...postdoctoral workforce in the sciences. The Dana Farber Cancer Institute (at Harvard) and MIT BCS (Department ... and sustainable research ecosystem and that we cannot ignore these changes if we wish to minimize the...

...tools so that both veteran and new Rett researchers can find the reagents and resources they need to cure...for the first time that programmable RNA editing can repair a gene in a living animal across multiple ...are available in the Resource Center. Researchers can also find links to cell line repositories including...models, Rett syndrome is a strong translational candidate for gene replacement therapy. In 2014, RSRT brought... the work of the consortium, two gene therapy candidates advanced into clinical development programs in...

...the brain. With the advent of optogenetics, ICSS can now be done with neuronal cell-type and temporal ...remote memory retrieval) specificity. Optogenetics can be used to more precisely associate regions of the...behavioral output with optogenetic manipulation, this can be explained primarily in two different ways 1) your...

...here for you as you learn the ropes! All material can be found on our Protocols page and YouTube page.  ...

...address these issues using our existing resources. You can read it, and other case studies, on the Seqera website...

...process doesn’t cause double-stranded breaks and can deliver large sequences and even entire genes. ...testing, and approval. In contrast, a system that can deliver a healthy gene as a single intervention for...integration occurs in a single enzymatic step. We can’t wait to see where evoCAST takes us on the high seas...

...oligomerization of biotinylated proteins on avidin can be controlled easier than formation of GST dimers...fusion protein from a bacterial extract. The MBP tag can be removed using Xa protease. The carboxyl-terminus...extent, the number of added biotins per molecule cannot be well-controlled. The lack of site selectivity...We are clearly NEB “fanboys” as the bacteria significantly enhance protein folding (tested using real science...

...enhance Collections so that a group or organization can annotate their sequences from a shared database of...companion product. If you don’t use SnapGene yet, you can download a free trial. If you notice that a feature...

... single field of view for constant tracking. You can then directly monitor multiple rounds of translation...contains 24 copies of SunTag, a synthetic scaffold that can recruit GFP fused to the SunTag-specific antibody...

...the most skeptical reader of historical fiction (I can personally attest to this!). Following history from..., we’ve read books across nearly every genre you can imagine: gothic, mystery, sci-fi, fantasy, pop-sci...

...to the plates to select for candidate knock-ins. These candidate animals can then be further screened based...made CRISPR in C. elegans even easier - now, one can generate a fluorescent protein fusion, transcriptional...

... growing conditions. As a facultative aerobe, it can thrive with or without oxygen. Additionally, its ...much more. Because of it’s BSL-1 status, E. coli can be used as a model to relate these processes in more...microinjections. Injections of mRNAs, for example, can be analyzed to understand human disease alleles during... norvegicus genes! Mexican axolotl (Ambystoma mexicanum) Ambystoma mexicanum, aka the axolotl. Image... Biosafety Level 1 microbe makes it a perfect candidate for introductory teaching and research environments....  Rattus norvegicus. Image from Staffan Vilcans. Rat physiology is also easier to study and ... Image from AJC1. The Mexican axolotl is one of the most unique model organisms, recognized for its...