Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 898 results for: CAN

Showing: 761 - 780 of 898 results

  1. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...cases, the selected mutation must be removed, which can be done by isolating animals with particular visible...become even more powerful following a report that one can use linear repair templates (ie. PCR-derived dsDNA...precise knock-ins, and homozygous knock-in animals can be obtained in eight days. The only animals on a ...2017). Because C. elegans has an AT-rich genome, it can be difficult to find an appropriate NGG PAM site ...repair of a temperature-sensitive pha-1 mutation significantly enriches for knock-in of 2x and 3xFLAG epitopes...

  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...itself a versatile option for tagging proteins, as it can be fused to the N- or C-terminus (or even in the ...., 2024 under CC BY license. This construct can be easily expressed and purified from E. coli, retains...light source, they have lower background signal and can be used in light-sensitive systems. Most excitingly...2024 American Chemical Society. Both PILATeS (18 kDa) and STReTCh (11 kDa) are significantly smaller... revealing antigenic differences between human, canine and simian isolates. J Gen Virol., 68 (Pt 11), ...expressing it with additional cofactors or using significantly truncated forms that limit its functionality...

  3. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...tools so that both veteran and new Rett researchers can find the reagents and resources they need to cure...for the first time that programmable RNA editing can repair a gene in a living animal across multiple ...are available in the Resource Center. Researchers can also find links to cell line repositories including...models, Rett syndrome is a strong translational candidate for gene replacement therapy. In 2014, RSRT brought... the work of the consortium, two gene therapy candidates advanced into clinical development programs in...

  4. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...general guide to the effect on higher education, you can see that those in teaching roles (such as adjuncts...communications with and research on these institutions, we can say with relative certainty that just over 40% of...at some institutions; and that institutions which can more easily provide bridge funding to absorb the ...postdoctoral workforce in the sciences. The Dana Farber Cancer Institute (at Harvard) and MIT BCS (Department ... and sustainable research ecosystem and that we cannot ignore these changes if we wish to minimize the...

  5. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...the brain. With the advent of optogenetics, ICSS can now be done with neuronal cell-type and temporal ...remote memory retrieval) specificity. Optogenetics can be used to more precisely associate regions of the...behavioral output with optogenetic manipulation, this can be explained primarily in two different ways 1) your...

  6. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...address these issues using our existing resources. You can read it, and other case studies, on the Seqera website...

  8. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...process doesn’t cause double-stranded breaks and can deliver large sequences and even entire genes. ...testing, and approval. In contrast, a system that can deliver a healthy gene as a single intervention for...integration occurs in a single enzymatic step. We can’t wait to see where evoCAST takes us on the high seas...

  9. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ... single field of view for constant tracking. You can then directly monitor multiple rounds of translation...contains 24 copies of SunTag, a synthetic scaffold that can recruit GFP fused to the SunTag-specific antibody...

  10. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...the most skeptical reader of historical fiction (I can personally attest to this!). Following history from..., we’ve read books across nearly every genre you can imagine: gothic, mystery, sci-fi, fantasy, pop-sci...

  11. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...oligomerization of biotinylated proteins on avidin can be controlled easier than formation of GST dimers...fusion protein from a bacterial extract. The MBP tag can be removed using Xa protease. The carboxyl-terminus...extent, the number of added biotins per molecule cannot be well-controlled. The lack of site selectivity...We are clearly NEB “fanboys” as the bacteria significantly enhance protein folding (tested using real science...

  12. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...enhance Collections so that a group or organization can annotate their sequences from a shared database of...companion product. If you don’t use SnapGene yet, you can download a free trial. If you notice that a feature...

  13. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...to the plates to select for candidate knock-ins. These candidate animals can then be further screened based...made CRISPR in C. elegans even easier - now, one can generate a fluorescent protein fusion, transcriptional...

  14. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ... growing conditions. As a facultative aerobe, it can thrive with or without oxygen. Additionally, its ...much more. Because of it’s BSL-1 status, E. coli can be used as a model to relate these processes in more...microinjections. Injections of mRNAs, for example, can be analyzed to understand human disease alleles during... norvegicus genes! Mexican axolotl (Ambystoma mexicanum) Ambystoma mexicanum, aka the axolotl. Image... Biosafety Level 1 microbe makes it a perfect candidate for introductory teaching and research environments....  Rattus norvegicus. Image from Staffan Vilcans. Rat physiology is also easier to study and ... Image from AJC1. The Mexican axolotl is one of the most unique model organisms, recognized for its...

  15. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...learn from other biotech companies at workshops and can use what we learn to improve processes in the Addgene...Without proper processing, these small DNA strands can form concatemers in later steps. This new NEB kit...biotechnology company that uses neoantigen biology to treat cancer. My partner for the day was Julie from Novartis...

  16. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...non-recoverable, the stock of the mAb becomes limited, which can be a threat to future reproducibility. The Trimmer...based system for expression of R-mAbs is that it can easily be engineered. Like humans, mice express multiple...

  17. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...in target cells. While the various AAV serotypes can be used to achieve different patterns of expression...tissue targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases...

  19. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...Elasticsearch, a powerful open-source search engine that can scale to search millions of web resources and handle... to a user? Moreover, there are many ways a user can input their query — by directly typing terms in a...

  20. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...OptoNB can bind, it co-localizes with its target at the cell membrane and when the OptoNB can’t bind, ...translocation. These results demonstrate that OptoNBs can be harnessed to regulate signaling pathways such ...signaling pathway, which is often over-activated in cancer due to its involved in cell proliferation and differentiation...
Showing: 761 - 780 of 898 results