We narrowed to 798 results for: Cre
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TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m....hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...
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Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....change gave her the balance she needed. It is incredibly helpful to me to know that you can make big changes... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....Well, it used to be a lab assistant, but with increased plasmid demand it was too time consuming to complete... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....parallel. More broadly, we drew on the combined creativity and expertise of the group and yet never hesitated... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m.... How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has Been Used for Lineage... -
Tips to Make the Most of a Scientific Conference
TypeBlog PostPublishedMay 23, 2019, 12:26 p.m....Twitter in real life. If there is not a tweep up, create one yourself. Pick a time (ex: lunch, happy hour... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for the membrane, where it will ... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m....17,560 CRISPR plasmids. That’s almost a ninety-fold increase in size! From this collection, we’ve shared almost... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m....were having a positive experience, I was still incredibly nervous about leaving academia. A lot of academics... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....starting vector that the depositing lab modified to create their new plasmid. The Addgene Vector Database... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....addition, there is a drop in image brightness with increasing depth caused by light scattering and absorption... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m.... the time it takes the FP to correctly fold and create the chromophore. This can be from a few minutes... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog PostPublishedMay 30, 2019, 12:44 p.m....improvements and adaptations that can provide an increased understanding of DNA binding and gene regulation... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....appears to return to normal. The model fit these two discrete phases with an estimate of 62.3% for the COVID... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....AAV-RPE65 treatment, famously visited Congress to help increase awareness about the potential of gene therapy!... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....makes sense that vectorization strategies include creating an AdV transfer plasmid with the E1 and E3 (Early... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....don’t have to optically magnify quite so much. Created with biorender.com Steps 2-4 are not found in...