We narrowed to 884 results for: IND
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TypeBlog PostPublishedJuly 2, 2015, 8:37 p.m....best poised to share the stories and difficulties behind great discoveries by tackling a research project... on generating blog content that scientists will find useful and interesting than that will simply get...
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The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog PostPublishedApril 15, 2014, 3:11 p.m.... little care I took with my discards and to be mindful of the lab materials I was using. I started to... These large, cheap aliquots could be stored indefinitely at room temperature (thus also saving freezer... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m....toxic in high amounts? It is always good to keep in mind that plasmids with low to medium copy numbers can... considerations are especially useful to keep in mind if you are planning to purify your plasmid DNA: ... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog PostPublishedSept. 17, 2020, 1:15 p.m....stormy times. It feels like we’re being buffeted by wind and waves and sometimes wonder if we can take another...SARS-CoV-2 would be fully open and available to industry requestors as well. This is a time when we need... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....inherently unstable due to their secondary structure, palindromic nature, and high GC content. This instability...expression in retinal cells Resources on Addgene.org Find AAVs for your experiment Browse our AAV protocols... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....neuroscience and is available to both academic and industry scientists. The collection of antibodies available...As we build the collection, we hope that you will find useful antibodies for your research. Please consider... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m....your selection method kills the transgene free individual. For example, with CRISPR-Cas9 gene editing,...experiments can only be collected from heterozygous individuals. Only 1/4 of the descendants of the heterozygous... -
Trading Spaces - Visiting Scientist Edition
TypeBlog PostPublishedAug. 22, 2023, 1 p.m....piece of equipment that is custom-built, hard to find, or inaccessible to you for another reason. Visiting...visiting position. What now? First, you’ll need to find someone who is willing to host you! If there is ... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....recombinant proteins by the pharmaceutical and biotech industries as a protein expression workhorse. This yeast...into methanol and peroxisomal autophagy can be induced by removing methanol from the media. This feature... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog PostPublishedJune 30, 2020, 1:15 p.m....little bit. You can just go to sessions that you find interesting and not go to the rest.” Having an on-site...blog Read all of our blog posts about conferences Find 25 tips for working from home ... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m....journals and publishers. As journal editors, we often find ourselves having to ask authors for additional information...Press. Additional resources on the Addgene blog Find more ways to make your research more reproducible... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog PostPublishedFeb. 9, 2021, 2:15 p.m....get to annotate things as they come up in their minds. The final screen can also be saved and exported...PhDs: Swimming upstream a career from academia to industry”. To date, aside from doing science, he has assisted... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....free to play around with different strategies and find out what works for your organization. Ask for feedback...Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a Research Project Read Other Inside... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m....Resources at Addgene.org Find Virus Protocols Visit Our Viral Vector Pages Find Virus for Your Research... -
A Deep Dive into BioBuilder
TypeBlog PostPublishedOct. 11, 2017, 1:44 p.m.... the only informal synthetic biology club of its kind, running from start to finish during the academic... Addgene.org Guide to SynBio Assembly Standards Find Plasmids for Creating and Controlling Gene Networks... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....system or by filtering by fly as species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector...experimentation. Drosophila are amenable to many different kinds of experimental assays depending on your interests... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....method it is not possible to follow turnover of individual proteins in situ. For the visualization of specific...Söderberg, Ola, et al. "Direct observation of individual endogenous protein complexes in situ by proximity... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m....request from us but also by high school students and industry scientists as a universal resource for information...about Addgene Resources on the Addgene website Find plasmids for your research Learn about our viral... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....the repressor because TALEs can be programmed to bind tightly to arbitrary DNA sequences and have been...100 fold repression in E. coli. In addition, TALEs bind non-cooperatively to the promoter region, which ...