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Showing: 761 - 780 of 853 results

  1. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... a variety of species, including the standard laboratory mouse. It’s also made targeted insertions relatively... mouse model. To avoid disrupting splice donor/acceptor sites, Miura et al. (2018) recommend targeting...

  2. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9 either through injection...Mediated Genome Editing with Adeno-Associated Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci...

  3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some cells...gift from Feng Zhang and is described in Improved vectors and genome-wide libraries for CRISPR screening....

  4. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...PA-GFP. PA-GFP was derived from Jellyfish (Aequorea victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine..."Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging." Nature methods...

  5. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg et al., 2014...conformational changes that make Cas9 such a valuable laboratory tool are still an area of active research. New...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...expected location of the target is an important factor in deciding which protocol is likely to work, we...more permanent knockdown, consider using shRNA vectors instead. With both siRNA and shRNA, it can be difficult...

  7. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...helped us manage our data. To quote Jason Niehaus, Director of Data Science and head of our Data Team, “Our...

  8. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...the names Aavery, Addy, and Cappy play on viral vector terms. And, the name Kendall has a special place...

  10. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...CRISPR, Addgene gave to me: An engineered prime editor with enhanced editing efficiency in plants 🎵 ...

  11. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ... Although we're storing away all of our holiday decorations, our holiday spirit remains. We would like...

  13. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...by email.  About the author Niko McCarty is the editor of Asimov Press and is designing a genetic engineering...

  14. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...Addgene Videos Page 5 Science Rap Videos and Their Creator: Tom McFadden Using Video to Share Your Science...

  20. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...Landscape for Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite...
Showing: 761 - 780 of 853 results