We narrowed to 789 results for: mpl

1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   Published

   June 2, 2015, 6:06 p.m.

   ...this post in the graphic below - including some simple comparisons. The easy multiplexing afforded by...

2. 3 Challenges in Plant Synthetic Biology

   Type

   Blog Post

   Published

   July 22, 2014, 5:46 p.m.

   ...cannot build what we do not understand, and the complicated and unique metabolic processes of plants are ...

3. Dos & Don'ts When Publishing a Scientific Manuscript

   Type

   Blog Post

   Published

   May 22, 2014, 4:02 p.m.

   ...peer review that occurs post-publication and is completely open; the reviewer’s name and affiliations, and...

4. The time and cost required to make a plasmid

   Type

   Blog Post

   Published

   March 23, 2023, 1:15 p.m.

   ...outlined in Table 1.   Of course, this is for the simple insertion of a PCR product into a vector. The time...

5. Lighting Up Cell Signaling with Photoswitchable Kinases

   Type

   Blog Post

   Published

   July 25, 2017, 1:20 p.m.

   ... for photoswitchable kinases.  Below are a few examples demonstrated in Zhou et al. 1. Testing kinase ...

6. Antibodies 101: Affinity Reagents

   Type

   Blog Post

   Published

   April 14, 2022, 1:15 p.m.

   ...camelid antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an alternative...

7. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   Published

   Aug. 5, 2021, 12:15 p.m.

   ...related to addiction and pain. Once these are completed, Hubbard will image these neurons using GCaMP7...

8. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ..., GFP linked the two GBPs together to create a complete transcription factor, activating luciferase transcription...

9. Career Coaching for Scientists: Why and Where Do I Find One?

   Type

   Blog Post

   Published

   Jan. 29, 2015, 1:25 p.m.

   ...? Some coaches focus on graduate students. For example Dora Farkas, founder of Finish Your Thesis (who...

10. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...cells, and animals. Some of these viruses are completely inert, but others can cause diseases. Nervous...

11. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ... into functional multi-subunit macromolecular complexes, but a serendipitous discovery led them to investigate...

12. Tips for Technical Support Calls

    Type

    Blog Post

    Published

    Sept. 20, 2016, 2:39 p.m.

    ...be fairly fast, getting the full answer to a complicated question may take some time. If the customer ...

13. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ...or be detected even if the two proteins form a complex. To help troubleshoot your experimental setup ...

14. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ...host/plasmid DNA during selection if it’s not completely bound up. While this likely occurs at a low rate...

15. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    Published

    Aug. 13, 2024, 1:15 p.m.

    ... own proteins, making the purification process simpler. Due to its high yields, E. coli is often used ...

16. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    Published

    Sept. 6, 2022, 1:15 p.m.

    ...Sharing data and community concerns AAV vectors are complex biological reagents; many factors, like storage...

17. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    Published

    May 19, 2016, 2:30 p.m.

    ...Lentiviral barcoding libraries have been used for complex applications like lineage tracing in heterogeneous...

18. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ...plasmid pEZY202  from Yu-Zhu Zhang’s lab) is an example of the former. In this system, histidine-auxotrophic...

19. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...of DNA and both identified lymphocyte antigen 6 complex (LY6A) as a receptor for PHP.B. Read on to learn...

20. Lab to Office Culture Shock

    Type

    Blog Post

    Published

    Feb. 5, 2019, 1:04 p.m.

    ...friend: “I'm in the process of onboarding a new employee who's coming directly from grad school. The transition...