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...important of those is to always look closely at the trace file (or chromatogram) of the sequencing results...sequence (no Ns in sight). If you never looked at the trace you would be happy. But look closer, the overlapping...

...blogged about before, there was the first direct demonstration that CRISPR's could be used to correct disease...30. (Pubmed) Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems. ...

...protein could be used to measure gene transcription, as well as to track protein localization. And as it turns...

...Campbell, and read about his "follow your bliss' mantra and never forgot it. Usually when I see inspiring...Addgene blog Read all careers blog posts Read our transferable skills guide Resources on Addgene.org Find...

...scientists and the public and help make science more transparent. Second, SITN helps graduate students develop...communication and organizational skills provide great training for a number of alternative careers in science...

...into 2025, these milestones remind us of the extraordinary impact that happens when scientists come together...simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged ...

...interests are very clear, I had no idea how to translate them into real world careers. I figured this internship...world, I was worried about how my ideas would be translated through Zoom calls and Google docs. I pondered...

... emphasizes Accountability, Consistency, and Transparency. This label scores products by their environmental...your RMV to the landfill, it’s collected and transferred to the Red2Green processing facility, where it...

... haven’t, go check it out!). After months of strategizing, designing, developing, testing - and repeat...redesign our homepage to reflect this message. We concentrated on what our homepage actually said, how we displayed...

...the green, red, yellow, cyan, blue, and orange spectra. A comprehensive list of cell markers all tagged...everywhere to get a clearer picture of that intracellular world, even as the Davidson lab prepares to ...

...spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study the expression...worked in the the UK, California, Africa, and Australia, viewing science as a global collaboration. This...

...target cells that have previously been stably transfected for Cas9 expression. The Yusa lab has used their...elements.” These lentiviral libraries and their demonstrated application offer proof-of-principle that CRISPR-Cas9...

...and more efficient. Our original policy was straightforward, but cumbersome: depositors had to email us...materials are both excellent ways to provide transparency, support reproducibility, and accelerate science...

...of University of Michigan says, “Addgene has transformed the way in which we share plasmids and other ...shipping logistics and facilitate all material transfer agreements, thereby freeing up time for our depositors...

... meet hundreds of the over 11,000 conference registrants, including scientists from six continents who...see below) on my way back from getting a badge illustrated with my favorite microorganism (Rhodobacter sphaeroides...

... different CRISPR applications in plants.     Transgenic yeast as source of dietary supplements Dr. Mrinalini... proteins. In this project she aims to create transgenic yeast as an alternative source of essential omega...

... your job title doesn’t change. Master’s level training can prepare you to present your work at conferences...a desk, although there’s often technical career tracks for PhDs who want to stay at the bench. So if you...

...explanation is given for how someone can or should demonstrate this quality.   I am often asked to give career...role of doer for your entire career. Scientists train as doers and often maintain an enjoyment in the ...

...application. Depending on the tag, directly imaging or tracking your protein may also be possible, without the...DOI: 10.1038/521274a Arribere, J. A., et al., Translation readthrough mitigation. Nature. (2016) 534, 719...

...sequences required for viral replication and transcription as well as reporter genes. To learn how you ...generating protein machines that can extend and retract over many microns in response to pH changes without...