We narrowed to 583 results for: BASE;

...system. Biosensors based on Nano-lantern technology are also very robust, with an eNL-based calcium sensor...RLuc8) with Venus. This luminescent technology is based on the principle of bioluminescence resonance energy...They also developed calcium, cAMP, and ATP sensors based on Nano-lantern, showing the technique’s versatility...

...time was not right for the development of phage-based therapeutics. After a brief stint at the venture...from developing therapeutics to developing phage-based tools for detecting pathogens in food products. ...biotechstart.org Read Sample 6's Paper on Bacteriophage-based Diagnostics Additional Resources on the Addgene...

...gravity-based column plasmid maxiprep kit takes 1-1.5 hours to complete, while this syringe-based method...Compared to a commercial magnetic bead-based kit, this cellulose-based method was faster, cheaper, and had... Addgene follows a similar workflow as a column-based plasmid extraction kit. First, you lyse the bacteria...

... let the virions out. We recommend using a Tris-based lysis buffer and sonication.  The obtained virions...particles that can transduce cells, which can vary based on target cells and can be altered by freeze/thaw...experimental outcome and planning.  Both ddPCR- and qPCR-based titering assays measure physical titers, meaning...post.  We generally do not recommend using ELISA-based titering assays, since those will quantify both ...Inactivation of Adeno-Associated Viral Vectors by Oxidant-Based Disinfectants. Human Gene Therapy, 32(13–14), Article...

...are many protein-based toxin-antitoxin systems. One of the most well known protein-based toxin-antitoxin...counter this, methyltransferases add methyl groups to bases at specific nucleotide sequences, preventing the...system that is regulated by antisense RNA, which base pairs to cognate mRNA to inhibit its translation...Crystal structure of the MazE/MazF complex: molecular bases of antidote-toxin recognition." Molecular cell 11.4...

...for studying colloidal gold (1).  The method was based on using a thin plane (sheet) of light generated...microscopes, optical sectioning of a specimen is based on discriminating the out-of-focus reflected light... combining Ultramicroscopy with tetrahydrofuran-based tissue clearing (13,14). A few years later, Karl...Deisseroth’s lab developed CLARITY, which is a hydrogel-based method that also allows antibody penetration for...recently developed AAV (adeno-associated virus)-based fluorescent labeling methods that can be used with...

...microbiome studies.  Find the MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota...microbiome, James Collins’s lab developed a paper-based method that can be used to detect microbes and biomarkers...As mRNA expression cannot be identified with DNA-based qPCR methods, the ability to detect mRNA transcripts...Takahashi, Melissa K., et al. "A low-cost paper-based synthetic biology platform for analyzing gut microbiota...

...slightly with either a 1-7 base poly(U)tail in tRNA-gRNA constructs or a 1-4 base tail from the second tRNA... cassettes (considering the gRNA itself is ~100 bases) limit the number of gRNAs that can be packaged ...Pseudomonas aeruginosa to cleave 4 gRNAs separated by 28 base Csy4 recognition sites from a polycistronic transcript...

...LoxP (locus of X(cross)-over in P1) sites are 34-base-pair long recognition sequences consisting of two...event. This event can have three general outcomes based on the location and orientation of the loxP sites...above, a genetic modification can be restricted based on experimental constraints. This has been used ...asymmetric spacer "NNNTANNN", where "N" indicates which bases may vary from the canonical sequence. Among these...

...options come in two categories: protein-based and chemical-based. Before selecting one, check your antibody...concentration. That makes the choice easy! Protein-based blockers include dry non-fat milk or serums like... of blocker, you can also use gelatin.  Protein-based blockers are inexpensive and easily made in the ... tip! If you get a speckled image, your protein-based blocker is either degraded or not completely mixed...mixed together.   Commercially available chemical-based blockers, while much more expensive, are consistent...to another. Since antibody binding varies wildly based on proteins, and their differing amounts of available...Smaller dynamic range; no multiplexing Protein-based blocker Degrades quickly Wet transfer Increased...

...mass spectrometry, and classified by source cell based on single-cell RNA-seq data. In addition to unique...valuable resources for future studies of the molecular bases of astrocyte-neuron interactions. Plus, the tools...Simon Grelet Lab developed MitoTRACER, a retroviral-based reporter system that permanently labels the cells...parent fluorescent proteins, PinkyCaMP is the first based on mScarlet. Compared to RCaMP3 and jRGECO1a, PinkyCaMP...FRCaMPi uses an inverted topology of FRCaMP (itself based on mApple, which means it likely has the blue-light..., Imai, S., et al. (2024). PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability...

...protein context, but they still depend on antibody-based detection or western blotting, methods that are ... tagged proteins and facilitates precise CRISPR-based tagging, or low-levels of ectopic expression, thus...models, and even introduce mutations. This vector-based use of HiBiT provides a means to quickly answer ...Bioluminescence detection is a cornerstone of modern cell-based assays and enables ultra-sensitive detection of ...challenges typically occurring with a fluorescence-based approach. These challenges can include background...degradation, trafficking (Hall et al., 2012). Antibody-based tools can also be combined with the HiBiT tag to...

...years, deaminase-mediated base editing (cytosine base editor or adenine base editor) and reverse transcriptase-mediated...in Zhu et al., 2019). Both cytosine base editors and adenine base editors have been developed for plants...Belhaj et al., 2013). Nuclease dead Cas9 (dCas9) based activators (such as dCas9-VP64) or repressors (dCas9...

...scientific field-based solutions or open source solutions. In comparison to paper-based record keeping,...notebooks and researcher's needs The familiar paper-based lab notebook is still a standard approach to record-keeping... or brainstorm, but we have to admit that paper-based record keeping barely copes with the growing amounts...future? As most payable software solutions have user-based pricing, it is good to know how many of your lab...

...process. Each variant is assigned a functional score based on its library frequency before selection compared...libraries of DNA fragments that contain variable bases (NNN) at any given codon site of interest can be... technique described in Wrenbeck et al is a PCR-based approach for generating a customizable comprehensive...primer set contains three consecutive randomized bases (NNN) at a given codon site and multiple primer ...Keith E J Tyo, and Timothy A. Whitehead. "Plasmid-based one-pot saturation mutagenesis." Nature Methods ...contain random synthesis errors, especially single basepair deletions. Therefore, Gibson assembly is better...codons that can be mutated? 88 codons, or 264 basepairs, was the maximum length tested by Wrenbeck et ...

...day, you should be happy to learn that many DNA-based reagents are named for soup ingredients! From pSOUP... intracellular RNA dynamics. Aptamers are 10-100 base nucleic acid oligos that bind with high affinity...that control the expression of coding RNA. Spinach-based riboswitches can be used as stable metabolite sensors...mimic of green fluorescent protein by fluorescence-based selection and directed evolution." Journal of the...PMC4244833. Kellenberger, Colleen A., et al. "RNA-based fluorescent biosensors for live cell imaging of ...

... modifications (~1-50 base pairs). Small edits require as little as 30-50 bases for each homology arm,...arm, but keep in mind these numbers may vary based on your locus of interest and experimental system.  Synthesizing...  Synthesizing ssODNs longer than 200 bases can be difficult, thus dsDNA plasmids are generally preferred...Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve HDR Efficiency ...

...latency (Bialek et al., 2016). Additionally, CRISPR-based activation can be made inducible upon environmental...through all the troubleshooting required for Cas9-based activation. CRISPRa for genome-wide studies One ...larger scale of activation allows for exploratory-based research as well. For example, CRISPRa can be used... interactions. Additionally, large scale CRISPR-based screens can be used to gain insight into protein...date his group has develop several popular CRISPR-based tools for altering DNA sequence or regulating gene...

...developed a video-based high-throughput screen for directed evolution of biosensors based on their dynamic...says Aggarwal. This biosensor changes fluorescence based on the release of glutamate from neurons and this...has developed new redox sensitive GFP2 (roGFP2)-based biosensors for live cells. His work focuses on the...

...amino acid linker to use. These decisions were made based on known functional aspects of the protein gleaned...sequence into which it is being inserted: one thousand base pairs on each side, in fact. Later, when we transfect...precautionary measure, it’s also good to make a few single base pair mutations in crRNA target sites found within...endogenous gene tagging systems. Addgene’s CRISPR-based Protein Tagging collection includes systems for ...

...will focus on one of the most common, purification based on the polyhistidine tag. What is the polyhistidine...tag was developed as a means to purify a protein based on the affinity of the imidazole ring of histidine...Bornhorst 2000).  One downside of polyhistidine tag-based purification, however, is the propensity for nonspecific... tags can also be used as a target for antibody based assays. Antibodies are useful tools for studying...

...Reporter signals may be electrochemical or light-based, with luminescent and fluorescent reporters being... domain induces changes in a fluorescent signal based on ligand presence or changing cellular conditions...Method Notes Quantification of a process Plate-based assays (plate reader) Detect the average amount...

...repository for DNA-based materials, including our popular kits and libraries. This DNA-based materials repository...

...initiating a base editor-based clinical trial for SCD in 2022 and Editas Medicine starting a Cas12-based clinical... today. Below we will review some of the CRISPR-based editing therapies that are currently approved or...

...called GMAP (Gibson assembly-based modular assembly platform). GMAP is based on the Gibson Assembly method... Cas9-based AID tagging Seven years ago, Masato Kanemaki's lab successfully used the plant-based auxin-inducible...University, we are now providing a powerful CRISPR-based screening source for the Drosophila community as... it is composed of two sub-pools. The first is a base “90k library” that contains ~90,000 gRNAs that target... biosensor A pH-sensitive fluorescent biosensor-based assay system was recently deposited with Addgene...University of Zagreb, constructed a CRISPR-Cas9-based tool for targeting CpG methylation by fusing catalytically...proteins in a target cell. In addition to nucleotide-based linking strategies, post translational protein fusion...

...robust protocol it does have limitations. The enzyme-based CUT&RUN method overcomes many of these limitations...interacting. The CUT&RUN method Enter CUT&RUN, an enzyme-based protocol for identifying DNA binding patterns that...sets, of which there are many readily available. Based on the success that our lab and others have had ...

...School, he developed the first lentiviral microRNA-based system for polymerase II-regulated RNA interference...virology,” he says. He was working in an HIV lab and based on that experience, soon directed himself to finding...months, the lab began a project to develop an AAV-based COVID-19 vaccine.  Vandenberghe’s work with AAVs...

...from some labs have developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine sensor... dLight1 sensor is a single fluorescent protein-based sensor. This sensor was engineered from a GFP variant...sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy: a GFP variant bound to a ...

... or other products in the suite. Since it’s web-based, you could access it from anywhere. Its ease of ...Biorender is like clip art for scientists. The web-based collection of over 20,000 icons is designed by professional...stop struggling to make figures in PPT 🔬 Also, the base version is free, forever 💜 https://t.co/KJpyxgxeQt...

... level with CellTag CellTagging is a lentiviral based tagging method that labels cells with heritable ...three-fold increase of fully reprogrammed cells based on single-cell gene expression patterns. The lab...Chuner, et al. "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics." bioRxiv...

...desktop-based software for plasmid design and mapping. ApE (A plasmid Editor): A free, donation-based plasmid...knowledge resource for cell lines IBEX Knowledge Base: A data repository for validation data on IBEX reagents...antibodies: A database of validated antibodies for mouse, human, and marmoset brain IHC.  FPbase: A data repository... Flame plasmid pET21a-BirA.   DNA / protein database search and analysis tools Addgene's analyze sequence...sites Open reading frame finder  Addgene vector database: Find sequences for plasmids assembled from publications...nucleotide or protein sequences to sequences in a database ExPASy translation tool: Translates a DNA sequence...sequence using all six open reading frames UniProt: A database of protein sequence and functional information...

...polymerase, the construction of specific sequences for base pair overhangs, insert and vector purification, ...specific to the insert with an additional 15-17 bases that are complementary to the overlapping vector...introduced into my inserts. However, with any PCR-based cloning technique there is a chance of introducing...

...tells us about a new tool for efficient multiplex base editing and multiplex prime editing (requiring minimal...dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles...

...therapeutic potential. Note: constructs discussed are based on Adenovirus type 5. Three generations of adenoviral...delivery: Potential applications for gene and cell-based therapies in the new era of personalized medicine...formation assay on A549 cells . Alternatively, antibody-based assays are commercially available for faster detection...

...American, Africa, or South Asian) used in the screens based on the cells’ germline genetic variations. They ...generating genetic dependency data with an ancestry-based bias.   The correction process does not stop there...genome-wide screens, introducing bias into all work based on these and any other CRISPR screens using the ...variation contributes to false negatives in CRISPR-based experiments with varying burden across ancestries...Cancer Dependency Map (DepMap), a cancer dependency database used to make this information accessible to the...

...short ncRNAs, such as microRNAs that are around 20 base pairs long. Using a single guide RNA (sgRNA) to ...Figure 1: A schematic illustration of CRISPR/Cas9-based approaches to study ncRNAs. (A) Transcription start...your experiments, there are plenty of other CRISPR-based options that can target ncRNAs.  CRISPRi and CRISPRa...10.1126/science.1225829  Hazan J, Bester AC. CRISPR-Based Approaches for the High-Throughput Characterization...

...packaging systems have been developed over the years based on HIV-1; however, the 1st-generation vectors are...defines 2nd- or 3rd-generation transfer vectors based on whether or not they have a chimeric 5'LTR. Second-generation...? What percent of the vector components are HIV-based? A7: No single plasmid contains all the components...insertional mutagenesis. These risks are largely based upon the vector system used and the transgene insert...

...While there are more and more molecular biology-based activities making their way into high school classrooms...BioBits ExplorerTM and BioBits BrightTM, biology kits based on freeze-dried, cell-free (FD-CF) components. Making...and is interested in developing synthetic biology based tools for science outreach and advocacy. You can...

...community.     Why recombinant antibodies? Animal-based methods for antibody production have several drawbacks..., 2015a, b). Recombinant antibodies are plasmid-based and offer numerous advantages, including more secure...Antibodies 101 blog post Learn more about plasmid-based recombinant monoclonal antibodies Resources on ...

...and jargon Hemingway Editor  This is a free web-based tool for detecting the readability of your writing...or dissertations. Phrases are categorized either based on the section of a manuscript they might be found...to work with collaborators. Zotero is a desktop based program that’s compatible with Mac, Windows, and...directly import papers from web pages and academic databases.  Downloading Mendeley Cite lets you cite papers...