...communication activities, e.g. tweet it when you publish a new blog post, and live tweet from conferences... If you live in the UK, join the SciComm mailing list. 7) Internships – Learn on the job Nothing prepares...internship at a magazine or newspaper (here’s a partial list). A great opportunity is the AAAS Mass Media Fellowship...into science communication by taking a media or journalism course. Courses can help you gain work experience...like Northwestern University or UC Berkeley have journalism graduate schools where you may take a science...research and residential communication. If science journalism is the path you want to follow, check out the... at the National Council for the Training of Journalists (NCTJ) or the Alan Alda Center for Communicating...

...introduced into the B. subtilis genome. Mobile-CRISPRi is then transferred from B. subtilis to the bacterium...colonies, but some bacteria, including E. faecalis and P. mirabilis, had only ~1 transfer per 1,000,000 colonies...CRISPRi into a single bacterial strain, such as a B. subtilis, or a narrow range of bacterial species, such ...integrative and conjugative elements in Bacillus subtilis (ICEBs1) system. ICEBs1 carries the genes needed...bi-parental mating. Following excision from the B. subtilis genome, the Mobile-CRISPRi ICE element forms a...integrative and conjugative element in Bacillus subtilis." Plasmid 86 (2016): 14-25. PubMed PMID: 27381852...

...the information. In contrast, a list of experiments or a laundry list of molecules to screen has unclear...Either way, think realistically about the time and resources required to feasibly accomplish the aims and ...proteins regulate the Z pathway?" is an open-ended list of proteins. Instead, consider "how do proteins ...exist to solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe...learned and/or now be possible to do? Research accomplishments are remembered for the problem solved, not ...chalk talk with questions from the committee. For a list of common questions, check out this eBook about ...

...complicated! Listen to the full podcast for more great science communication tips or listen to the chapters...topics discussed during the interview. Happy listening! Listen to the interview with Science in the News ...Pint series. Chapter 4: The SITN Podcast, Sit'N Listen - In their podcast, the SITN team brings in scientists...like to develop the SITN podcast as a means for listeners to understand the dynamics of information flow... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...stupendous listener, but I aspire to  be. If you don’t at least aspire to be a good listener and consciously...brought to the table was empathy, a willingness to listen, and an ability to remember the right stories at...disagreeable, you recognize their feelings as valid. Listening: In one of my favorite books, Michael Ende’s Momo... nearby village (and to the reader) by simply listening. Chances are, you know someone like this, even...Ende, courtesy of morefamousquotes.com A good listener absorbs and remembers and makes the teller feel...collaboration may not be the easiest resolution to accomplish, but there is a potential for long-term gains...examples of times when you have used your empathy, listening skills, and institutional knowledge to resolve...

...annurev-neuro-111020-100834 Challis, R.C., Ravindra Kumar, S., Chan, K.Y., Challis, C., Beadle, K., Jang, ...or lumbar spine. Image and caption courtesy of Challis et al., 2022. Where do they come from? Systemic...Addgene’s Data Hub and the CLOVER repository of published systemic AAVs to find commonly used vector cargo...capsid, while Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids...https://clover.caltech.edu. Figure adapted from Challis et al. 2022. Image created with BioRender.com. ... plasmids available from Addgene Capsid Published Tropism Model Organism Mouse Strain-Specific...Strain-Specific Tropism?" column. For a complete list of mice strains with LY6A-dependent tropism, see...

...and why other publishers and organizations have various different versions of checklists and guidelines...communication falls on the shoulders of journals and publishers. As journal editors, we often find ourselves ...contributions, etc.) before their paper can be published. To a scientist, all of this checking and formatting...communication further up the collective priority list. There has been progress—for example, a number of... of other Cell Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she...reproducible Read all of our blog posts on scientific publishing  ...

...citations in its first four years (1). In addition to publishing in Science, Professor Zhang deposited the associated...CRISPR/Cas papers and Addgene deposits, but papers published in other years also get many more citations This...For example, it is very plausible that science published in more widely-read journals gets more citations...adjust our estimates based on the journal that published the articles.  That is, we re-estimate the citations...associated with Addgene deposits as if they were published in the same journals as those that did make deposits...papers with Addgene plasmids in our data were published in Nature, but only 3 percent of papers without... Weighting Example Article Number (A) Year Published (B) Number of Citations (C) Journal Name (D) Percent...

...Imaging Improving Well-established High-throughput assays Improving Well-established Biological sources...firefly(Photinus pyralis) 560 61 Luciferin ATP No Japanese firefly(Luciola cruciate/lateralis)Italian firefly...organism for most people is the firefly (Photinus pyralis) and perhaps not surprisingly it is also the most...describing the cloning of firefly luciferase was published in 1985, several studies utilized luciferase as...luciferase isolated from the firefly beetle (Photinus pyralis) is the most frequently used bioluminescent reporter...

...for controlling molecular processes with light. Listen to Our Segement on PhoCl Segment  Hot Plasmid 2...selection for increased CRISPR editing efficiency. Listen to the Segement on Ouabain Selection  Hot Plasmid...base editors with enhanced range and specificity. Listen to to the Segmennt of Improved CRISPR Base Editors...Plasmids for generating DNA molecular weight markers.  Listen to the Segement on the pPSU Plasmids Special thanks... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer....     Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie...

...cells). pBACKBONE-XXXXX Tip: For a catalog of published and commercially available empty backbones, visit...fusions that are on your insert. Typically you would list any tag or fusion protein in the order they appear... tag on the N-terminal of your insert, you would list it first. pBACKBONE-Flag-hGene If there was also...fused to the C-terminal of your insert you would list it after the insert. pBACKBONE-Flag-hGene-EGFP Other...included in the plasmid name. Mutations are generally listed as the amino acid change and not a nucleotide change...proper way to denote an amino acid mutation is to list the one letter abbreviation of the wild type amino...

...Pasteurella multocida, Porphyromonas gingivalis, Proteus mirabilis, Providencia spp., Pseudomonas spp.,...stability, which influence the plasmid’s ability to establish and maintain itself within host cells.  The plasmid...bacteria in lab manipulations. Table 1 has a detailed list of the bacterial species these plasmids are compatible...compatible with.  Table 1: Non-exhaustive list of common broad host range plasmids and the bacterial species...Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia stuartii, Pseudomonas spp., Rhizobium...campestris, X. campestris pv. malvacearum, Zymomonas mobilis pBBR1 based pBBR1  Gram-negative  Alcaligenes...

...2013 there have been 33 CRISPR software tools published and documented by OMICtools. It must be confusing...The first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple ...sgRNA designs. The second database, CrisprGE (published 27 Jun 2015), is noteworthy for its broad scope... content, and ease of access. The third, WGE (published 15 Sep 2015), is more similar to EENdb than CrisprGE...in detail, but that is already covered in the published articles. Instead, I have broken down the tools...or another. Supported organisms: This section lists the genomes supported by each tool. The genomes ...agnostic and have currently available organisms listed in this section. CRISPR software advice for specific...

...conversation. Goals and Planning Worksheet: Lists accomplishments, research/professional goals and feedback...credit for what we have accomplished. Starting with a recap of accomplishments, from learning new techniques...time has gone. Are we any closer to graduating, publishing that key paper, or figuring out a career path...tangible research and career goals, and celebrate accomplishments. These techniques are laid out in Vincent et...

...Organizing your lab notebooks, protocols, and to do lists can be easier than you think! Here are three tips...the colors and text. Tip 2: Use electronic to-do lists to access your tasks on the go Never miss a deadline...everything to your calendar. Ditch the paper To Do List and move to one that moves with you. Use software...software such as Evernote or Google Tasks to create lists of your upcoming tasks, set deadlines, and arrange...attachments. Organize your notes into notebooks, create checklists that link to other notes, and use the search ...spreadsheets for reagent storage locations, such as the list used to organize your cryo freezer. No more issues...

...Jessica Polka from Harvard Medical School and a long list of supporters from all of the research centers in...problems resulting from past practices and mores.  His list is dire:  The current system is unsustainable, hypercompetition...be radically overhauled.  Finally, the science publishing system needs to be changed.  Dr. Bourne charged...training excellence to begin effecting change.  Panelists call for action Each of the speakers in the panel...applause when he talked about how he boycotts publishing in all but open access journals.  Dr. David Glass...astounding lack of reproducibility being recognized in published work even in the “best” journals.  He pushed for...

...we'd like to extend a special thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...asked leading professors for their top 3 tips. Tom Ellis 1. Keep up with the literature. He recommends setting...too; both with your research and with getting published.” Connie Cepko 1. Work hard. There will be periods...mentees who have goals outside of the university? Ellis recommends that a student interested in leaving ...engineering (STEM) expertise in law, business, journalism, entertainment, politics, K-12, etc.” Addgene's...Acknowledgments Thank you again to Connie Cepko, Tom Ellis, and George Church for answering our burning questions...

...for Microbiology (ASM) Scientific Writing and Publishing Institute. Writing is the cornerstone of any ... them with the world. If one doesn’t write and publish in some way, then the data produced isn’t likely...manuscripts and the ASM Scientific Writing and Publishing Institute (SWPI) is an extremely beneficial guide...should possibly take a composition class in the English Department. He suggested that I sign up for the...the SWPI online course because a course in the English department wouldn’t have trained me in the scientific...Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific Manuscript Writing Scientific Manuscripts...

...magazine. Humans are built to enjoy, remember, and listen to narratives — take advantage of that in your ...stories — whether I’m working with a talented journalist to shine light on living as a scientist in Beijing...research struggles in papers. They recount accomplishments in talks and interviews. They even tell short...tell good stories, they engage their readers or listeners thus expanding the impact and appreciation for...Interviewee: “I did the most work for a paper we were publishing, but when it came time to add author details ...which had affected his judgement. Eventually, we published the paper in a high-impact journal and it worked...

... a neuroscience major, I was struck with the realisation that I didn’t want a career in neuroscience—whatever... to once again write for fun eventually saw an English minor tacked onto my BSc. With this decision I ...towards a career in science communication, an established field of inquiry with which I was not yet familiar...meaningful day job only came into focus when an English tutor noted my eclectic mix of science and arts... a scientific background and bring with them established interests and areas of expertise. Perhaps the... research explores risk-taking behavior among collision sport athletes, as well as the ethical and health...

...she would be looking at where I had published, who I had published with and whether I would be able to... set up an ELISA kit company on whose website you can find some great information on ELISA troubleshooting...Undergraduate sections and brought in more focus on the list of conferences I presented at and placed them under...troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts...

...and foster an effective working environment? Tom Ellis was offered a start-up package that included a postdoc... most surprising thing about becoming a PI? Tom Ellis has a one word answer: “Email. Nothing prepared ...differently or the same as your adviser did? Tom Ellis merges the influence from his two PhD advisers who...who were “chalk-and-cheese”. While one was an established Professor who was always “off flying his plane...our latest results as soon as the gels ran.” Dr. Ellis tries to lead his team as a scientists and a friend... lab!”   Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

...pre-clinical life science work, economics) that published research is difficult to replicate.  Why is replicating...appreciate how few details are provided in most published papers. The precise timing, order, or every combination... rarely exist or are preserved as part of the publishing process and become lost if not archived. Remove... article is given “in-principle acceptance” to publish the final results regardless of the outcome of ...null findings that are notoriously difficult to publish.  With Registered Reports, the authors are able...scientific research. These include policies for publishers and funders in the Transparency and Openness ...

... their precise positions within the plasmid. The list of enzymes that can be detected is 5x greater than...GenBank File or a SnapGene file for the sequences listed. You can then analyze the sequence in your sequence...Sequence” tabs. Features The table found here lists the common features detected in the plasmid sequence... map, direction (if relevant), and types. Types listed are standard GenBank feature types. Clicking on...Sequence” tabs. Primers This tab contains a table listing commonly used primers detected in a given nucleotide...Analyzer guide A quick guide to all of the tabs listed above and their functions. We hope that these...

...recent plasmid deposit please sign up here.      Listen to this episode of the Hot Plasmids podcast Light-mediated...transmission Article contributed by Klaus Wanisch  Listen to the optogenetics podcast segment An optogenetic...and joined each fragment with one part of an established pair of proteins (iLID and SspB) that undergo...mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment In...regulation Article contributed by Michael Lemieux  Listen to the miRNA reporter podcast segment Discovered... CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast segment Understanding the...

... plasmid deposit please sign up here.          Listen to this episode of Hot Plasmids! Optogenetic control...by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast segment Anti-CRISPR ...using blue light (Bubeck et al., 2018). They accomplished this by inserting a photo-sensitive LOV2 domain... Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast segment The neurons... system Article contributed by Michelle Cronin  Listen to the 2in1 cloning podcast segment Recently, the...and AsLOV2 Article contributed by Angela Abitua  Listen to the photoswitchable protein podcast segment ...

... Map & Network (CeNGEN) The CeNGEN goal is to establish a comprehensive gene expression atlas of every...publication for the worm community where one can publish brief novel findings. These results are normally...results. This a great resource for scientists to publish findings that don't fit into larger publications... that can be referenced. In addition the data published in microPublications is curated and added to other...but there are Drosophila and zebrafish articles published there as well.   Worm Development Dynamics Database...used for introducing point mutations that was published in a microPublication (Schwartz and Jorgensen,...

... impossible to interpret. Polishing the fiber optics with diamond polishing paper of sequentially increasing... second key point; the fiber optics need to be polished to significant smoothness to prevent drastic loss...its output with a light meter before and after polishing. Efficiency of the implant should be at least ... output. The same considerations apply to the polishing and preparation of the patch cable (although you...it’s best to try those that have already been published first. Because behavioral optogenetics is performed...

...intellectual property, it worked away in isolation. It published a paper a year (or less) and limited the exchange...public and to other academic labs. Being unable to publish or attend conferences makes it very challenging...As a Master’s student, she had less pressure to publish and more freedom to attend conferences, but after...occasionally thinking about how we could bypass the establishment hiring system altogether. We had a lot of ideas...restricted to science jobs. Keira’s an optimist, I’m a realist, and together we make a pretty good team. We have... – although we didn’t figure out how we would accomplish the color change until a few months later. RevBio...

... can be helpful. Publishing: As a postdoc at AstraZeneca, you are expected to publish, but this can vary...prerequisite “industry experience” that I’ve seen listed as a requirement on some industry job postings....vary depending on which company you choose. If publishing is important to you, make sure that your project... Regeneron, and Genentech, among others, and the list is growing. Some post positions as they come up,... Novartis, have specific web sites for postdoc listings, so the method of posting varies between companies...

... thanks to our guest bloggers Alissa Lance-Byrne and Alex Chavez! Alissa Lance-Byrne is a graduate student...This post was contributed by guest bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss...gene or introduce a desired genetic alteration. Listen to Our Podcast Interview with Alex Chavez ... induce Cas9 to make edits (AC, WLC, and JQ, unpublished results). An additional benefit of the use of...PMC4265475. Additional Resources on the Addgene Blog Listen to Our Interview with Alex Chavez Learn about Sequencing...

...access to a list of posters until the day-of, take a moment to step aside to read through the list at the ... a day in advance. Dress for the venue and professionalism of the conference, and remember to wear comfortable...about the relationship of your work to recently published papers or “hot topics,” so be sure to be well-...strategically use your time. Don’t have access to a list of posters at all? No problem! At the beginning ...traverse the sea of posters If you come prepared with a list of posters of interest, then you’ve already set ...

... new accomplishments to add. Obtain copies and translations of documents that are not in English. Create...development, consulting, policy, big data and the list goes on and on. As you think about your career (... to obtain permanent residence. The recently established case Matter of Dhanasar provides the three-prong...qualify, you need to satisfy 3 out of the 10 criteria listed below. An international or national award for ...therapeutics to cure the cancer. Authorship:  Publishing books, journals online and/or in print. Having...demonstrate outstanding achievement Evidence of published material in professional publications written ...

... already answered this question and used it to publish!  Working with AAV vectors in neuroscience Let...performance in our system and yet none of that data was published.    Fig. 1: Testing of three different ... for your system or to successfully replicate published works. Of particular importance to these efforts...build on others’ results is available not through published manuscripts, but through word of mouth or old ...asked your team members or colleagues: How much unpublished AAV data do you have sitting in computers and... O’Connor, D. M., Lutomski, C., Jarrold, M. F., Boulis, N. M., & Donsante, A. (2019). Lot-to-Lot Variation...

...made a table that listed each protein in the class, then for each protein we listed all the studies that...include every piece of information that was ever published. Obviously this isn’t possible. So, define your...people cared about. I also used reviews to get a list of research papers that I needed to read. Once I... page as possible. This could be in the form of lists, streams of consciousness, or anything else. Like.... Curate and present some useful data Typically lists and pictures are the most useful parts of reviews...

...specific subjects in the interview. Happy Listening! Click here to listen! Podcast breakdown: (Time : Topic) ...Harvard Medical School researcher Connie Cepko. Listen to the podcast below to hear all about the research... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer... Plasmid Tools Being Developed in the Cepko Lab Listen to our Interview with Michael Koeris Get Cepko ...

...get people to remember you). What better way to accomplish all of these things naturally than to give a ... talk without looking at their slides or notes. Listen to their stories and narratives (see below “Tell...presentation appropriately.  Find out who will be listening.  Are they early, mid, or late career?  Will many... Tell a story We are biologically programmed to listen and remember better when we hear material delivered...should be very simple. The goal is to get the listeners to be thinking “What happens next?”  Describe .... During your practice rounds, encourage your listeners to ask questions and record those that were asked...

...endogenous homologous recombination enzymes can also accomplish the desired genomic modification. This technique... which results in a merodiploid. This can be accomplished by selecting for an antibiotic resistance marker...counter-selection system relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is...binding site (using the online calculator from the Salis group) (Espah Borujeni et al., 2014). Replaced the...scarless modification of bacterial genomes. It can accomplish large deletions, insertions, and even precise..., Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation rate is controlled by coupled trade-offs...

... Video Production Specialist and Arthur Guerra who is a Customer Support Specialist. Both of these Addgenies...Hola! Que tal? My name is Brandon Solis, and I am a rising senior studying Sociology and Dance at Skidmore...of challenging what I am used to. Brandon Solis, marketing intern. Something that I struggle ... Brandon Solis...

...which he submitted a phony paper to a list of 304 open access publishers. By the time the magazine went to...world. 4)      The Annals of Internal Medicine published systematic reviews on an agent used to promote... into account. 7)     Science’s John Bohannon published an article describing a sting operation in which... publications, although many don’t necessarily publish in OA journals themselves. A good number of those...