Skip to main content
Addgene
Showing: 121 - 160 of 314 results

  1. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic potential in mice with a dominant...component, CAR. Clinical trials are underway to explore CRISPR’s capacity for both classes of edits in ...

  2. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...ll dive into those questions in this post as we explore… the life and times of the myc tag.  Origins of...that their new monoclonals would help the field explore Myc’s role in cell proliferation and tumorigenesis...

  3. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...quickly. Of course, you might have less time to explore scientifically since you can’t afford to spend ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly...

  4. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins and RNase...concentration of a chaotropic salt (guanidine hydrochloride for the plasmid purification protocol and guanidine...

  6. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...but also alter an individual’s native, beneficial flora. Currently, there is no antibiotic strategy available...population, represented here by cells of different colors, specifically targets bacteria with the given genomic...

  7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...processes. Re-engineering GFP to increase its range of color and application Almost as soon as its sequence... not listed here, many permutations within each color also exist with only slight variations separating...

  9. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ... their selection techniques to come up with new colors (Fig. 3), such as Radish and Carrot, and further... ligands. References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human ...

  10. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...other disciplines, or from other countries, to explore new connections to our research. So how can we ... 525.7569 (2015): 306-307. 3. Spicer, Scott. "Exploring video abstracts in science journals: An overview...

  11. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...’ll choose to study Crepidula atrasolea or Prochlorococcus with your next project. Gymnotiformes and Mormyridae...evolution in immunology, neurobiology, and more. Prochlorococcus (Penny Chisholm) This cyanobacterium is the...

  12. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...into the content of Addgene’s aptamer soup by exploring the development and applications of fluorescent...aptamers Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described in...

  13. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...experiment and choice of loading control. First, let’s explore why these assumptions are key to using loading ...loading control. Thanks for joining us in this exploration of western blot normalization. May your blots...

  14. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion Don’t just plan to reuse...about the particular audience and venue before tailoring each presentation appropriately.  Find out who...

  15. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...specific nucleotide substitutions (or deletions) in a tailored manner. The approach can be used in conventional...plasmids, but may also facilitate genome editing. Tailored mutations are commonly introduced to endogeneous...

  16. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...fluorescence for fluorescently labeled probes. Colorimetric and luminescence based detection methods are...and behavior.     References 1. Southern, Edwin Mellor. "Detection of specific sequences among DNA fragments...

  17. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...of carbohydrate binding proteins waiting to be explored and exploited. With better characterization, these...Jessica C. Sacher, and Christine M. Szymanski. "Exploring the interactions between bacteriophage-encoded...

  19. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... work The Island of Dr. Moreau (1896) where he explores the concepts of animal testing and modifications...but the common themes that these authors were exploring were space and robots. Warping to stars in space...

  20. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...fluorophores can add a second layer of contrast, color contrast, to an image and thereby provide molecular...Microscopy Choosing Your Fluorescent Proteins for Multi-Color Imaging Choose the Brightest Fluorescent Protein...

  21. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...effector — Cas9, Cas12, or Cas13. If you’d like to explore Class 2 CRISPR in more depth, the Jennifer Doudna...8(1). https://doi.org/10.1186/s13578-018-0255-x Taylor, H. N., Laderman, E., Armbrust, M., Hallmark, T...

  22. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...Rhodamine 123 (R123) (Liu et al., 2017). In multi-color imaging, it is essential to select dyes with different...applications. Other current advancements include work exploring far-red-emitting dyes in bacterial imaging, offering...

  23. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...antibodies (i.e., immunofluorescence), or by a colorimetric reaction facilitated by an enzymatic reaction...Cruse JM, Lewis RE, Wang H, Geziena MT, Steven GE, Lorna J. (2004) ANTIGENS, IMMUNOGENS, VACCINES, AND IMMUNIZATION...

  25. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...Traditional tracing compounds either produce a colorimetric product or are fluorescent. While dyes usually...ratios, making these neurons fluoresce in different colors. An individual neuron can then be traced throughout...

  27. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ...never forget! The Mackin Lab from University of Colorado -Anschutz Medical Campus (@CSD_CUAMC) caught a...

  28. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...impossible, due to their small size and inconspicuous coloring.  FLIS plasmid Recently, the Phuels lab deposited...

  30. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...coincidentally matches our Christmas tree's RGB colors) 😅🎅🤶🎄👨‍🔬👩‍🔬 pic.twitter.com/y34Tv8VCLT—...

  32. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...best costume takes home the ultimate prize – a glorious trophy. Sure it’s plastic and you have to share...

  34. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...over 70 orders!), we sent Outreach Scientists to Colorado, Berlin, Atlanta, Ottawa, Norwich, and Hannover...

  35. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...downloading my plasmid request data, I started to explore how I could visualize the aggregated data. Plotting...

  36. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...Addgene Leadership team in all their photo booth glory! #1millionplasmids On to the next million! @CarolineBME...

  37. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ... by field (ex: microbiology, neuroscience), or explore other areas such as scientific sharing or publishing...

  39. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...used feature: the search bar. We’ve changed the colors and centered it on the page, so your eyes will ...
Showing: 121 - 160 of 314 results