We narrowed to 488 results for: mme

...section on a plasmid webpage for any specific recommendations from the depositing lab. If your vector is ...recombined product tend to grow faster, so we recommend picking the smaller colonies which are more likely...

...-19 research, cures, and diagnostics. Almost immediately we began to receive urgent requests for access...make most of our payroll for the spring and early summer months. We are also extremely grateful to the grant...

...HMS and has been involved in a variety of commercialization efforts in gene therapy either through direct...industry collaborations, grants, or setting up commercial and nonprofit companies. His lab focuses on gene...

...Being an entrepreneur is difficult; it requires an immense amount of self-knowledge and an unwavering devotion... process in order to develop those ideas into commercial products and next generation companies.  We launched...

...change can be observed under a microscope using commercially available beam splitters. In addition to imaging...tendency to shy away from ratiometric imaging, he recommends they give it a shot. “Beginners sometimes think...

...launched the Michael Davidson and Roger Tsien Commemorative Travel Awards in 2017. These travel awards are...this year’s Michael Davidson and Roger Tsien Commemorative Travel Awards: Melissa Chiasson, Elizabeth Unger...

...Apoptosis or “programmed cell death" plays a pivotal role in an array of biological processes including...PMC3790201. Elmore, Susan. "Apoptosis: a review of programmed cell death." Toxicologic pathology 35.4 (2007...

...reagents, several journals or organizations now recommend or require authors to use specific PID formats...Addgene_87155)” Please note that we only provide recommendations for how to cite using the PIDs in the material...

...experiment achieving the gRNA representation recommended in the publications associated with these libraries...our viral services. Please send any questions or comments to [email protected]. Finally we’d like to thank...

... this is less of a concern, but Addgene still recommends plating the transformation mixture on solid media...interest about library use, let us know in the comments! Additional Resources on the Addgene Blog Check...

...launched the Michael Davidson and Roger Tsien Commemorative Travel Awards. These awards are intended to ... first two Michael Davidson and Roger Tsien Commemorative Travel Awardees: Xin Zhou and Fatima Enam. There...

... the project we are focusing on what we call “asymmetric collaborations.” These are transient partnerships...protocol can save weeks of trial-and-error. A recommendation for the correct statistical test can save a...

...promoter results in high R-body expression  (Schrallhammer et al 2012). This made them an appealing target...3759909. PubMed Central PMCID: PMC213417. 2. Schrallhammer, M. et al. Tracing the role of R-bodies in the...

... that future hires will look up to." She also recommends to seek out advice from seasoned PIs who can ...it is once it gets fully up to speed.” He also comments that it is also surprising “ how easily people...

...syringe."Feel free to share your alt text in the comments! We learned a lot from listening to others' alt...people.  Whether you start small with alt text or immediately dive into applying these four steps elsewhere...

...with biorender.com. ITR structure  WT ITRs are symmetrical 145-nucleotide sequences that flank the ends ...through ITR sequences. GC tails are often located immediately outside of the ITRs in the plasmid backbone and...

...guest blogger post about colony PCR, I signed up immediately. I had used colony PCR before and thought it ...your research or career? Share your story in the comments below!   Additional resources on the Addgene...

...section. Analyze your plasmid sequence For more immediate, high-level analysis, you can click directly on...us know what you think about the updates in the comments section below and keep checking in at blog.addgene.org...

...These modifications can be done in-lab using commercially available kits or ordered as synthesis modifications... to do-it-yourself with a kit or purchase it commercially synthesized with a label. Not enough options...

...plasmid sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids to confirm they contain...samples that are requested by scientists, will immediately be screened by our QC scientists to confirm the...

...a variety of stakeholders from industrial and commercial operators, government agencies, active researchers...scientific innovation amongst school children through summer workshops and interactive discussions. Not only...

...engineering is the fact that Cas9 can be easily programmed to make a DNA double strand break (DSB) in the...impact repair is that the Cas9 protein doesn’t immediately release from the broken end after cleavage, which...

...terminus. With that new design in place, Konermann immediately saw an order of magnitude improvement in activation...pooled libraries in your experiments, Addgene recommends that you review the steps for how to use pooled...

...your connections post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn users notice...turns a stranger into a friendly colleague. Recommended Reading & More: Scientist Networking: What is...

...particularly for applied projects intended for commercialization. Many of the genetic pieces developed over...able to overcome them. Share your stories in the comments below! And if you have developed a genetic tool...

...complementary skill sets, and “talk a good game." Mike recommends that the team should initially include a visionary... an idea or product? Please let us know in the comments section! And read on for more great information...

...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...use in your experiment! As a starting point, we recommend checking the literature and our AAV data hub to...

...consider leaving a link to its sequence in the comments below. References Sanes, J.R., & Yamagata, M....supernatant is only $40, significantly cheaper than commercially available antibodies. ...

...student. For post-grad school individuals, Susan recommends starting a blog, but with one caveat: “I hate...Association of Biomedical Research. She does, however, recommend using Twitter as a way to leverage unpaid work...

...from — Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the curve...introductions to lots of molecular biology,” — Reddit commentor Yay4sean, in r/molecularbiology.   Oh, ...

...resistant to the toxic effects of CcdB. Another commercially available, CcdB-resistant strain is ccdB Survival...these resources further. Feel free to offer your comments and suggestions, and even to submit a guest blog...

...increase capture. Longer histidine tags are also recommended if you need a very pure sample. Because binding...antibody, like this one developed by Dr. James Trimmer’s lab, to perform assays that they otherwise would...

...their bulk can be a barrier at times. Smaller, slimmer affinity reagents can have deeper penetration into...Single-domain antibodies (sdAbs), sold under the commercial name nanobodies, are derived from a camelid antibody...

...are always required and lab coats are either recommended or required in BSL-1 and required in BSL-2 labs...with lentivirus, many organizations require or recommend disposable gowns over your lab coats and double...

...regeneration-related genes in four inexpensive and commercially available planarians: Girardia sp., Dugesia ...IPTG-induced expression to work, students have to use a commercial E. coli strain that is designated as DE3. For...

...just right for your experiment. As always, we recommend checking your cloning via a miniprep and restriction...component plasmid, component name, size, and any comments. You can use the ID to look up the component plasmids...

...past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor/ ...not; applications for funding from charities, commercial organizations, or universities may have someone...

...that this has precipitated increases to the NIH recommended pay scale – used by many institutions to set ...PMC4304227. 4. Revitalizing biomedical research: recommendations from the Future of Research Chicago Symposium...

...antibody. Manning et al. tested a variety of commercially available secondary antibodies and found that...(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary...

...Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

... with our Financial Operations Specialist, Lucy Hammer, about why she did not go. She told me, “I think...favorite response to this question came from Lucy Hammer, our Financial Operations Specialist. She said,...

...come to a meeting with a clear agenda, you will immediately get your supervisor’s attention so you can focus...supervisors might try to intimidate you with negative comments (hostile-aggressives, complainers, know-it all...

... Highly expressed markers should be paired with dimmer dyes so they don’t “wash out” the rest of the panel...anything (negative population). It is strongly recommended that when you first run a new panel on a spectral...

...Addgene Blog - Leave suggestions and tips in the comments section below or contact [email protected] if you'd...scientific community on NgAgo forums, blogs, or in the comments section below. Keep pipetting and happy genome...

...believe that industry postdocs, having already been immersed in an industry environment, have a better understanding...or you want to end up in an industry job, being immersed in that environment sooner rather than later can...

...fragments, or genomic DNA. Also remember that commercially available cloning kits can often make the process... some that rely solely on PCR, and a host of commercially available cloning kits that one could choose...

... liquid containers closed when they aren’t in immediate use. This will prevent spills, but, if any spills...occur, they should be wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution...

...poured onto the middle of the plate, where it immediately spreads out to form a firm monolayer. The cell...the level of endogenous expression. We highly recommend the use of an electronic pipettor with variable...

...during the application process. Many institutions recommend that you reach out to potential advisors before...professors per program. Most applications will recommend that you name two professors in your application...

...hoping that your lab mate would have time to make comments on your manuscript. If you give her this slot,...separately in a future post.   Resources Maria recommends: Getting to Yes: Negotiating Agreement Without...