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We narrowed to 79 results for: Blue fluorescent Protein

Showing: 61 - 79 of 79 results

  1. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...map created in SnapGene of Blue Flame plasmid pET21a-BirA.   DNA / protein database search and analysis...brain IHC.  FPbase: A data repository for fluorescent proteins and their properties.  Expasy: Bioinformatics...with Addgene. NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database ExPASy translation...six open reading frames UniProt: A database of protein sequence and functional information, such as expression...

  2. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...of a protein, a yes-no plot is the best-case result. For example, you have introduced a GFP protein into... of a protein while the green (top) has an upregulated expression (shift to the right). The blue (third...adjusted voltage, cells that did not receive the GFP protein are most likely to be clustered at signals below...not! When you are looking to study the level of protein expression, instead of its presence or absence,...These smears tend to occur when you are looking at proteins that are expressed regularly in cells and might...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations...The orange peis the baseline expression of the protein of interest. Created with biorender.com. The...

  3. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...researchers, from selective protein ligation through introducing ncAAs with protein modifications of interest...If you study proteins, you’re probably quite interested in the canonical 20 amino acids. But in your ...your quest to learn more about your protein of interest, you may find the available amino acids a bit…limiting... called unnatural amino acids) are placed in a protein site-specifically to alter its function, mimic ...biologically relevant modification, or add in fluorescent or chemically reactive side chains, all of which...relationships or, in more practical applications, allow a protein to be more effectively used in a technology or ...put all these together, the cell will produce a protein with a site specifically incorporated ncAA, ready...

  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...mini counterpart label nearby proteins with biotin. The biotinylated proteins can then be isolated and identified...for Glypicans DNA editing with STITCHR Dynamic protein labeling with TurboCas Enhancer-AAV viral preps...transporters. The intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by ...primary visual cortex (V1) layer 2/3 (L2/3). B) Fluorescence from axonal boutons of neurons expressing iGluSnFR3...our bacterial plasmid set for expressing tagged proteins, and stay tuned for mammalian expression and AAV...therapeutic potential. Our partners at the Institute for Protein Innovation (IPI) recently released a set of highly-specific...antibodies against three of the six human glypican proteins (GPC1, GPC3, and GPC4). All IPI antibodies undergo...

  5. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...for the amount of protein you are expecting.  Fluorescent proteins Fluorescent proteins, while more expensive...binding to your protein of interest. Protein preparation Before you can blot your proteins, you’ll need ...normalize your protein of interest to overall protein expression for each sample and protein. While ubiquitously...normalize your protein of interest to total protein expression. Total protein expression can be measured using...rely on primary antibodies conjugated to fluorescent proteins, instead of the more common chemiluminescent...consider the subcellular location of your protein. Proteins located in membrane-bound subcompartments,...considered if your protein is difficult to solubilize, such as a membrane-bound protein. There are many ...

  6. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...blasticidin, neomycin, puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors...inverted repeats (green) flanking the gene of interest (blue) in a plasmid backbone. A separate plasmid contains...

  7. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...co-localization immunofluorescence, cell-type specificity. Viruses that only express the fluorescent proteins are ...Thankfully the Deisseroth constructs coexpress a fluorescent protein, which allows for easy identification of ...vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and cre driver mice. Current...optogenetics plasmids! Find AAV resources! Find fluorescent protein resources!    ...table above) include Channelrhodopsin2 (Chr2), a blue-light sensitive non-specific cation channel, activation...general rule of thumb, if you can detect a strong fluorescent signal (without antibody manipulation), then ...

  8. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    Published
    July 1, 2025, 1:15 p.m.
    ... are trying to remove from your sample is protein. Proteins absorb light to varying degrees across the...specific to proteins and why researchers traditionally use the A260/280 ratio as an indicator of protein contamination...and likely contaminants found in DNA samples. The blue lines in B-D represent the spectra for the specified...downstream experiments. The usual suspects, such as proteins, phenol, or guanidine salts, exhibit their own...how different contaminants impact these ratios.  Protein contaminants Whether you are purifying plasmid ... The A260/230 ratio can be a good indicator of protein contamination, but a number of other potential ...A260/280 ratio does not drop significantly until protein levels exceed 70% of the sample content (Loughrey...

  9. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... express one of a few different fluorescent proteins (red, green, blue). If a researcher injects a cocktail...and neuron-specific transgene expression. Fluorescent proteins are widely used for tracing and their expression...Maday et al., 2014). It is currently unclear which proteins facilitate transport of neuronal tracing compounds...either produce a colorimetric product or are fluorescent. While dyes usually spread unidirectionally, ...biotinylated dextran amines (Veenman et al., 1992) or fluorescent molecules like rhodamine-isothiocyanate (RITC... For predominantly retrograde tracing, albumin protein labelled with horseradish peroxidase (HRP; Kristensson...germ agglutinin WGA, Schwab et al., 1978) or fluorescent molecules such as Fluoro-Gold (Schmued and Fallon...

  11. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...expresses a unique combination of red, blue, and green fluorescent proteins in each state. The three methods...amount of information regarding which regulatory proteins, signaling molecules, mutations, and environmental...mentioned above (Sanger sequencing, qPCR, and fluorescent reporter-based) each come with their advantages...opinion, the most convenient strategy is the fluorescent reporter based method since it lends itself to...

  12. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...pMAS. Fluorescent proteins (FP, e.g. GFP) can be used in many ways. Often a FP is fused to a protein of ...enzyme cleaves a substrate, which will then form a blue precipitate. GUS is usually the first choice for...bioluminescence. Luciferases usually have shorter mRNA and protein half-lives then GUS and are therefore good temporal...elucidate the subcellular localization of your protein of interest. Find all plasmids from the Lee-Parsons...plants using syringe agroinfiltration." Recombinant proteins from plants. Humana Press, 2009. 41-50. PubMed...

  13. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...absence of a protein, determine if a protein is up or downregulated, examine a protein’s stability or ...Hjelm, 1972, Bjorck 1984). Protein A, Protein G, or the recombinant Protein A/G are conjugated to agarose...support while unbound proteins are washed away.  While both Protein A and Protein G have an affinity for...compatible with either Protein A or G.  While commonly used, Protein A and Protein G are not suitable for...applications, such as protein isolation from serum. In this case, Protein A and Protein G will bind both the...of the target protein or interfere with protein interactions of interest. Tagged proteins are typically...modifications, or study how a target protein interacts with other proteins or nucleic acids. Read on to learn...

  14. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ... such as when a plasmid contains certain fluorescent proteins. Finally, for many plasmids you can find...ordered more than 20 times; red, more than 50; and blue, more than 100 times! When you search for plasmids...

  15. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...molecule, the antibody binds to a protein, and then voila! Wherever your protein of interest is, a signal will...molecules to identify each protein of interest, this gel-based method identifies proteins solely by weight and... isn’t giving you enough signal to detect your protein of interest, it may be time to look at the indirect...antibodies. While a primary antibody binds to the protein of interest, a secondary antibody binds to the ...signaling molecules associated, indirectly, with each protein of interest.    Figure 1: The direct ...approach (right). Primary antibodies are represented in blue; secondary antibodies are represented in purple;... molecules are represented in light green; and proteins are represented in orange. Created with BioRender.com...

  16. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...homology dependent repair by enhanced green fluorescent protein (EGFP) reconstitution. (a) Scheme of validation...incubation, you assess the level of fluorescence in your cells under a fluorescence microscope. The higher the ...containing sgRNA sequences into HEK293T cells. The fluorescence intensity was classified into four groups (4...darker than control, 1; very dark). Brighter fluorescence indicates a more successful gRNA design. Adapted...your target is inserted, disrupting the EGFP fluorescent signal, for details on this plasmid, see Mashiko...the amount of fluorescence, the better that gRNA is at causing a double stranded break at the target site...screening as little as 8-10 pups. The level of fluorescence you saw in the validation assay should give ...

  17. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...method for identifying protein-protein associations. This method uses a bait protein fused to a biotin ligase...PrM N and C   Detecting protein-protein interactions with DULIP Proteins rarely act alone. Molecular...which “bait” proteins are tagged with Protein A-Renilla luciferase and the “prey” proteins are tagged with...with Rosella, a pH-sensitive fluorescent biosensor A pH-sensitive fluorescent biosensor-based assay system...ligase to biotinylate proximate proteins in the cell. These candidate proteins can be enriched via biotin ...of fusion proteins, requires less biotin, and shows enhanced labeling of proximate proteins. Overall BioID2...BioID2 improves the efficiency of screening for protein–protein interactions. The Roux lab has deposited HA...

  18. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters (orange).... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...exciting for scientists working to visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling...

  19. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ... BirA, has been used to identify and study protein-protein interactions in proximity labeling techniques...extensive research history and characterization. Many proteins and processes naturally found in E. coli have ...and a tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms including... Arabidopsis thaliana flowers. Image from BlueRidgeKitties. If you are working with Arabidopsis check...
Showing: 61 - 79 of 79 results