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We narrowed to 208 results for: CIL

Showing: 61 - 80 of 208 results

  1. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...Biological Sciences Research Council (BBSRC) and Medical Research Council (MRC) require authentic cells...

  2. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...by long terminal repeat (LTR) sequences, which facilitate integration of this portion into the host genome...vesicular stomatitis virus G protein (VSV-G), which facilitates viral entry into the host cell.  Transfecting...

  3. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... Rab35 to understand their roles in forming the cilia that help establish left-right organization of the...2023). Rab8, Rab11, and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer ...

  4. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... of other organisms, such as sphingomonads and bacillus.  ADH1 The ADH1 negative repressible promoter ...synthetic cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303...

  5. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...polymerase prior to fragmentation can greatly facilitate downstream manipulations. Phi29 polymerase is...your membrane to several freeze/thaw cycles to facilitate cell lysis: alternating between 5 min at -20°...

  6. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...inverted repeats separated by a loop region that facilitates the formation of a hairpin RNA structure, resulting... of long hairpin RNA (hpRNA). This method is facilitated by DNA constructs that enable insertion of ~400...

  7. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...should remove much of the unedited “haystack”, facilitating recovery of edited animals. Figure 1:... for C. elegans genome engineering have also facilitated CRISPR editing in C. elegans. These modular toolkits...

  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...2xNLS) and c-terminal (Cas9(C)-2xNLS) fragments facilitates target DNA cleavage upon co-expression and spontaneous...therefore have resistance to both kanamycin and ampicillin (from the pUC19 backbone). *False positives can...synthase pair is encoded on a single plasmid and facilitates the incorporation of a variety of bio-orthogonal...

  12. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...Clonetegration could become a “valuable technique facilitating genetic engineering with difficult-to-clone ...combinations (depending on the number of copies). This facilitates the distinction of neighboring cells in imaging...

  13. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...recombination target sequences, denoted by triangles] facilitate tissue or cell type specificity. An on/off genetic...administration of novel engineered AAV capsids facilitates enhanced transgene expression in the macaque ...

  14. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ... Addgene functional, handling everything from facilities management to website development, tech transfer...

  15. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ...Hub in this blog post. Alongside our efforts to facilitate the sharing of reagents and data, we’re also ...

  16. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...maintenance tasks. Hot tip: make sure your PI/facilities are okay with the decorations and that you’re...

  18. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ... convenience. After an order is placed, we’ll facilitate the necessary MTA, then start production and ...

  19. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ... the lookout for new ways to help researchers facilitate and accelerate scientific discovery. How can ...
Showing: 61 - 80 of 208 results