... Rab35 to understand their roles in forming the cilia that help establish left-right organization of the...2023). Rab8, Rab11, and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer ...

...polymerase prior to fragmentation can greatly facilitate downstream manipulations. Phi29 polymerase is...your membrane to several freeze/thaw cycles to facilitate cell lysis: alternating between 5 min at -20°...

... of other organisms, such as sphingomonads and bacillus.  ADH1 The ADH1 negative repressible promoter ...synthetic cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303...

...inverted repeats separated by a loop region that facilitates the formation of a hairpin RNA structure, resulting... of long hairpin RNA (hpRNA). This method is facilitated by DNA constructs that enable insertion of ~400...

...should remove much of the unedited “haystack”, facilitating recovery of edited animals. Figure 1:... for C. elegans genome engineering have also facilitated CRISPR editing in C. elegans. These modular toolkits...

...double-strand breaks (DSBs). DSB repair by HDR facilitates in-frame insertion of tag-encoding sequences ...efficiency across different stem cell types. This has facilitated easy generation of clonal knock-in lines that...

...Nashville, TN, USA 36.1627°N 86.7816°W- Jennifer Stancill I am a research biologist by training, so of ... larger and stronger that I expected. Jennifer Stancill, Ph.D., is currently a post-doctoral fellow at...

...communications, medical writing, compliance, core facility management, collaboration management, grant administration...help a candidate get looked at and should help facilitate the interview process. I often suggest to international...

...2xNLS) and c-terminal (Cas9(C)-2xNLS) fragments facilitates target DNA cleavage upon co-expression and spontaneous...therefore have resistance to both kanamycin and ampicillin (from the pUC19 backbone). *False positives can...synthase pair is encoded on a single plasmid and facilitates the incorporation of a variety of bio-orthogonal...

...Clonetegration could become a “valuable technique facilitating genetic engineering with difficult-to-clone ...combinations (depending on the number of copies). This facilitates the distinction of neighboring cells in imaging...

...recombination target sequences, denoted by triangles] facilitate tissue or cell type specificity. An on/off genetic...administration of novel engineered AAV capsids facilitates enhanced transgene expression in the macaque ...

... Addgene functional, handling everything from facilities management to website development, tech transfer...

...Hub in this blog post. Alongside our efforts to facilitate the sharing of reagents and data, we’re also ...

...maintenance tasks. Hot tip: make sure your PI/facilities are okay with the decorations and that you’re...

...interest in generating Cas9 based tools to enable facile control of DNA and RNA.     Special thanks to our...

... the lookout for new ways to help researchers facilitate and accelerate scientific discovery. How can ...

...approaches; (4) encodes a Thy1.1 selectable marker to facilitate the isolation of modified cells; and (5) is compatible...

... convenience. After an order is placed, we’ll facilitate the necessary MTA, then start production and ...

...Seeding Labs hopes to expand its activities by facilitating connections to mentorship and networking opportunities...

...distribute over 65,000 antibody units per year from our facility at the University of Iowa. The minimal fees from...