We narrowed to 118 results for: ENDOG
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TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....been to either systematically delete/overexpress endogenous genes or to introduce heterologous genes, but...
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K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....cinch. This is because it doesn’t secrete its own endogenous proteins, making the recombinant protein its ... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....also demonstrated the ability to be used as an endogenous RNA imaging tool. Fig. 1: Csm, shRNA... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....allows for whole organism imaging. They created an endogenous ApoB-NanoLuc reporter by inserting the NanoLuc... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....useful for therapeutic applications in vivo where endogenous anti-AAV antibodies often neutralize the therapeutic... -
DAP arrays for multiplex gene editing
TypeBlog PostPublishedJuly 7, 2022, 1:15 p.m....expression, the individual gRNAs are released by endogenous tRNA processing machineries, specifically, RNaseZ... -
Viral Vectors 101: Viral Vector Elements
TypeBlog PostPublishedJuly 25, 2023, 1 p.m....Of note, recombinant adenovirus is missing the endogenous E1 gene, making it replication deficient. This... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m.... that can replicate and divide along with the endogenous DNA. AAV also has low immunogenicity, meaning... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m....levels of gene activation for both reporter and endogenous genes. It is highly specific in mammalian cells... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....chemogenetics as they are relatively insensitive to their endogenous ligand, acetylcholine, and are only activated... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....also envision eNLs as good reporter genes for endogenous protein fusions created using CRISPR/Cas9. eNLs... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....recommends using an anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....animals that are genetically altered to overexpress endogenous RNAi pathways. These mutations however can interfere... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m....purification as well as reversibly regulation of endogenous signaling activity in cells. Let’s shed some... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....which restores eye color to fly lines where the endogenous white gene is knocked out. Flies with your integration... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Xie K, Minkenberg B, ... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....complex for the transcriptional activation of endogenous genes and it consists of three components: 1.... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m....the IP landscape, using plant parts which are endogenous to the plant, off patent, or not patented, and... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....proteins are most widely used tags because they emit endogenous fluorescence that allow easy detection with the... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....Giovanni Pezzulo, and Joshua M. Finkelstein. "Endogenous bioelectric signaling networks: exploiting voltage...