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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 306 results for: GAI

Showing: 61 - 80 of 306 results

  2. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...stated, ccdB makes cloning easier by selecting against vectors that did not take up your insert. But exactly...antitoxin protein (CcdA) that protects the cell against the toxic CcdB. Cells that lose ccdA through the...

  3. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...PhDs Later this week, postdocs and students will again gather in Boston - this time with the aim of finding...University, has recently published an article arguing against reducing the number of graduate students accepted...

  4. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...media or journalism course. Courses can help you gain work experience and build your network, but don’...Schmidt! Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas as a Marie Curie...

  5. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...the run completes, we begin our assembly process. Again, thanks to our partners at seqWell we make use of...failed” and removed from the plate to be prepped again. These holes are then filled with other samples ...

  6. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...called Geneious. Here, the raw reads are mapped against a reference sequence, if one was provided. If no...plasmid, and the full QC process is started over again after receiving the replacement plasmid at Addgene...

  7. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...that.” That was interesting. If people could be against GMOs as food but support GMOs that result in a ...business training. The money is a drop in the bucket against the funds needed to build a flower that changes...

  8. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...collection of CRISPR tools has grown steadily, gaining approximately 2,000 plasmids/year from 2017 to ...most popular with our requestors. However, we can again see quick adaptation and steady use of all types...

  9. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...negative selection marker HSV-tk is used to select against random recombinants.   Design your targeting...recombination, the negative selection marker selects against improper, random recombination into a different...

  10. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...comfortable with the open-ended nature of research, they gain confidence and learn to work independently, which...External collaboration results in student learning gains and positive STEM attitudes in CUREs. CBE Life Sciences...

  11. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...with 10 sgRNAs in the sgRNA metrics, confirming again that sgRNA design plays a significant role in library...specific genes allows scientists to conduct genome-wide gain of function screens. These screens can help reveal...

  12. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ... possible to screen libraries of bait and prey against each other at the same time in order to try and...reporter gene in eukaryotic cells, fusion proteins must gain access to the nucleus. To circumvent this requirement...

  13. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ... turned to characterizing its cleavage pattern. Again, they were in for a surprise! Whereas Cas9 generates... of the PAM may give Cpf1 the ability to cleave again and possibly mediate HDR. This “second chance” mechanism...

  15. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...-LV [+M], negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein antibodies...middle panel). Samples were also immunoblotted against vinculin as a loading control (right panel). Image...

  16. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...allowing us to reduce the genetic footprint yet again. This also led to improved expression homogeneity...ultrasound imaging. If needed, resort the cells again for improved purity and contrast. For in vivo experiments...

  17. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...eluted.     In the first method, an antibody against a target protein is immobilized, or tethered, on...variation, called a pull-down assay, an antibody against the tag is used to isolate the protein of interest...

  18. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ... code, we may need additional clarification and again we collaborate with the Product Team who in turn...would be like re-inventing the wheel over and over again for every single software today that prints labels...

  20. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...giving you a plethora of opportunities to explore! Again, attending one of these fairs is a great start, ...sure to get that person’s contact information and again, make a personal connection! Even if you don’t get...
Showing: 61 - 80 of 306 results