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We narrowed to 102 results for: Gene Tools Blue

Showing: 61 - 80 of 102 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... cassette to the gene of interest. The gene to be expressed is amplified using a gene-specific forward...CRISPR-Cas9 system for endogenous gene activation Optogenetics is a powerful tool that utilizes light to control...successful in regulating gene transcription using DNA-guided optogenetic tools; however, targeting the ...10.1073/pnas. CRISPR-Cas9 optogenetic tool for activating endogenous genes Moritoshi Sato's Lab has ...plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool from the...sgRNA-targeted gene, resulting in increased activation of endogenous transcription of the target gene. The brilliant...system. The tool allows scientists to use light to spatially and temporally control endogenous gene activation...

  2. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...with Addgene. Here, we take a closer look at the construction and validation of some of these tools created...their recombinant affinity reagent tools to include nanobodies. At Addgene, we are excited to be part of this...involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters (orange)....first antibodies distributed by Addgene.  The plasmids used to generate these antibodies were based off... used to generate cDNA, which  was used as a template for PCR amplification with a degenerate primer set...the Addgene blog:  Plasmid-Based Recombinant Antibodies  Plasmids 101: Secondary Nanobody Toolbox Antibodies...Introduction to Antibodies Resources on Addgene.org  Browse Addgene's recombinant antibody plasmid collection...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...understanding of B. subtilis gene functions and interactions. The single-gene deletion library is derived...studying the phenotype of an essential gene, the CRISPRi Essential gene Knockdown library was constructed ...protein PhoCl expands optogenetics toolbox Increasingly popular Optogenetics tools use light to alter cell...mpeg1.1), and astrocytic (gfap).This toolbox adds new neuronal tools to the expanding gateway compatible...viral approach Genetically modified organisms like transgenic mice are important tools for understanding...Bacillus subtilis libraries with Addgene – the B. subtilis Single Gene Deletion Library—Kanamycin and the...plant species. This toolkit includes TALEN and CRISPR nucleases, nickases, and gene activation systems ...

  4. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...region of the gene they are trying to disrupt or delete, which both inactivates the gene and acts as a... Resources on the Addgene Blog Read other Plasmids 101 Posts Learn How to Use Blue White Screening to...Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think...maintain the plasmid. Adding an antibiotic resistance gene to the plasmid solves both problems at once – it...Historically, antibiotics have also been used to disrupt genes at the chromosomal level. Scientists introduce an...typically has two separate resistance cassettes and your gene of interest is cloned into/inactivates or completely...resistance!  What are antibiotics? Antibiotics are generally defined as agents that kill bacteria, or inhibit...

  5. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...CRISPR toolbox. Cpf1’s staggered cleavage pattern opens up the possibility of directional gene transfer...from this publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex Gene Editing by CRISPR–Cpf1 Using... restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly helpful for targeting...mismatches on modification by AsCpf1 (red) or LbCpf1 (blue). Mismatch positions are indicated below each graph...26411297. Resources at Addgene Find CRISPR plasmids at Addgene Read Addgene's CRISPR Resource Guide View...Cas12a. In 2015, Zetsche et al. added to the CRISPR toolbox with their characterization of two Cpf1 orthologs...Again, they were in for a surprise! Whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1...

  6. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is a sucrase... in the chromosome is to disrupt expression of a gene with an antibiotic resistance cassette. This leaves...sometimes interfering with expression of surrounding genes. However, there are ways to create scarless mutations...Red that contained one of eight bacterial toxin genes isolated from E. coli or Psuedomonas aeruginosa....toxins, and phage holins. They hypothesized that genes that had evolved specifically for toxicity would... pathogenic Proteobacteria. We placed the toxin genes under control of a rhamnose-inducible promoter, ... versions of pTOX that encode one of two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm ...

  7. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...preps contain biosensors, optogenetic tools, and chemogenetic tools: protein tools that are activated, respectively...available at Addgene from 2016–2023 by type of tool. B) AAV preps distributed by Addgene per year from...  Figure 2: Numbers of AAV tools, by category, available from Addgene as of 2023. A large percentage...to its inherent bias: Addgene uses a prioritization process to decide which tools we make available. This...viruses (AAVs), will know just how tricky these tools can be to use. AAVs, which can target different ... 2016–2023. Tools and targeting The amount of vectors available and number of requests tell only part...sensors, are the broadest category of viral vector tools, which may help explain why they are the most commonly...

  8. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...targeted gene will be removed from the chromosome and and replaced by the resistance gene.The gene is thus...strategies to investigate a gene's function is to inactivate, or "knockout", the gene by replacing it or disrupting...endogenous gene. Here, we'll describe the techniques and the plasmids used to inactivate specific genes in mammalian...given gene.   Figure 1: A knockout targeting vector designed to insert a resistance gene. The ...targeted gene have been cloned into the vector on either side of an antibiotic resistance gene. To avoid... resistance gene through the insertion of flanking LoxP sequences, you can remove the gene through the...later remove a resistance gene. In this construct, the neomycin resistance gene is floxed so that it can...

  9. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ... transcription of a target gene, it therefore determines the timing of gene expression and largely defines...synthesis and allowing reporter gene transcription to take place. Addgene also has popular light-regulated...inducible tetracycline ON (Tet-On) system, a versatile tool developed for use in prokaryotes and eukaryotes,...the promoter and ceases repression of the target gene. A simplified lac inducible system is found in many...shock-inducible Hsp70 or Hsp90-derived promoters, in which a gene of choice is only expressed following exposure to...inducible promoters Light is another way to activate gene expression, and two-component systems used in synthetic...transcription. Red flame plasmid pDawn contains the blue-light sensing protein YFI. When light is absent,...

  10. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...essential for neuroscience research. The use of genetic tools for visualizing and perturbing circuits together...virus (AAV) has been widely used to deliver genetic tools to the brain because of its low cytotoxicity...nucleus AAVs CREATEd for gene delivery to the CNS and PNS Resources on Addgene.org Find the Spaghetti Monster...with the development of methodologies to deliver genes to the CNS have recently made it much easier to ..., and the long-term expression of AAV delivered genes. AAV serotypes have been extensively studied in ... fluorescent proteins such as tdTomato or RFP or blue fluorescent proteins like Cyan. The issue with these... are an excellent add-on to the neuroscientist’s tool-kit for studying the brain and we looked forward...

  11. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ... the world, it can be hard to find your favorite gene, promoter, serotype, or a myriad of other things... to highlight the convenient ways our new search tool lets you access plasmids from journal articles. ... useful plasmid, but what’s popular? The coveted Blue Flame is more than an award for depositors and inspiration...Retroviral) Species: Specify the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid Type: Choose...to find plasmids matching your search terms with gene and guide RNA / shRNA inserts Resistance Marker:...Resistance: Use this to choose the antibiotic resistance gene(s) used for selection in bacteria. Has Viral Service...stumped? Ask an Addgenie The newly launched search tool is a great improvement for our website and we hope...

  12. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...powerhouse tools combine for dynamic protein labeling By Alyssa Shepard To fully understand how genes are regulated... listened! One of the most powerful tools in a scientist's toolkit is the empty plasmid backbone: the ...pages or in the Addgene Antibody Data Hub. Perhaps these antibodies are just the tools you need to push...the NGR vectors for most experiments. These new tools promise to be a popular choice for a variety of ... Strong expression: A CMV promoter drives robust gene expression in mammalian cells. Flexible tagging:...tag. Selectable and stable: A puromycin resistance gene makes stable cell line creation a breeze. Modular...retroelements." The Abudayyeh-Gootenberg Lab developed this tool based on several new site-specific retrotransposon...

  13. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...there is a myriad of tools this could be applied to. When it comes to altering gene expression, CRISPR ...most popular choices for gene silencing. RNA interference RNAi is a useful tool to investigate the roles...roles of specific genes or to knock down genes with potentially harmful mutations in therapeutic settings...target gene but have a lot of off-target effects. However, this is a common issue with many gene silencing...of study for improving these tools. Next time you need to knock down a gene, maybe you’ll consider using...Additional resources on the Addgene blog RNA Interference in Plant Biology: New Tools for an Old Favorite CRISPR...101 Posts Additional resources on addgene.org Mammalian shRNA Tools for RNAi Molecular Biology Reference...

  14. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...Collection — scientists are always coming up with new tools to measure their favorite targets and finding new... directions that were inaccessible with earlier tools. They also produce more than 20 other MTRIA sensors... fluorescent properties, pYtags are an appealing tool for monitoring RTK signaling.  Figure 4: Design...in glycolysis, so this sensor will be a valuable tool to study cellular physiology and metabolism under...outperforms previous designs. B-gTEMP is excitable under blue light (470-495 nm) and is capable of local temperature...Viral Service pages to find more. We hope these tools will be useful in your future research, and we’re...new to Addgene this year. Feel the love with MTRIA sensors One of the latest additions to Addgene’s catalog...

  15. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...that quantitative PCR (qPCR) has been a powerful tool for quantifying AAV for some time now. When titrating...should have a clear separation between positive (blue) and negative (gray) droplets. The no template control..., and Eriko Uchida. "2D droplet digital PCR as a tool for titration and integrity evaluation of recombinant...recombinant adeno-associated viral vectors." Human Gene Therapy ja (2019). PubMed PMID: 31140327. PubMed Central...vector genome titers by droplet digital PCR." Human gene therapy methods 25.2 (2013): 115-125. PubMed PMID...found that generating the standard curve for qPCR can be challenging to get right. Here at Addgene, we use...droplet quality. Using a droplet generator, the sample and the droplet generation oil are moved through small...

  16. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... Figure 1: Left: recombined prime editing tools PE2max-NC and ePEmax3 optimized for dicots. NC: ...Right: T1 tomato plants that inherited an edited gene to confer resistance to the herbicide chlorsulfuron...hippocampal neuron expressing pan-membrane ASAP5. Solid blue circle marks the initiation site and open circles...mouse monoclonal antibodies targeting the human genes and are recommended for use in immunohistochemistry...phospho-Tau). We hope these will become valuable tools for your research! If they do (or if they don't!...contributing to the Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...

  17. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...Positive-selection vectors using the F plasmid ccdB killer gene”. Gene. 1994 Oct 11;148(1):71-4. PubMed PMID: 7926841... experiments, and here we will focus on a unique gene that makes the popular GatewayTM method possible...accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed it to...cloning technology. CcdB: A potent toxin... The ccdB gene, located on the F sex factor plasmid of E. coli,...and ultimately causing cell death. ccdA, another gene found in the ccd operon, codes for the antitoxin...desired clones.   ...Becomes a powerful cloning tool Molecular biologists first saw the potential of ...vectors, called pKIL18 and pKIL19, contained the ccdB gene in frame with an MCS. E. coli that were transformed...

  18. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    Published
    May 11, 2021, 1:15 p.m.
    ...University School of Medicine. Optogenetics and chemogenetics are powerful tools to modulate the activity of...(bottom). The images show examples of dendrites (blue shades) in the monkey amygdala. Red and green arrows...References on Addgene.org Check out Addgene's Chemogenetics Guide Find fluorescent proteins at Addgene ...on the Addgene blog Browse Fluorescent 101 blog posts Read about chemogenetics vs. optogenetics Find more...neurons and other brain cells. Since the opsins or chemogenetic receptors used in these techniques cannot be...versatile method to identify the expression of transgenes of interest. Furthermore, fluorophore variants... important influence on the expression of the transgene of interest and its localization in the membrane...

  19. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...:   Color Base FP Description Article PI Blue EBFP2 EBFP2(1-10) and Capri(1-10) for use with GFP...variant of mScarlet Split-wrmScarlet and split-sfGFP: tools for faster, easier fluorescent labeling of endogenous...including NanoLuc®, DULIP, LuTHy Other split protein tools: Split-TurboID for protein interactions (Alice ...resources on the Addgene blog Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors...where should you begin? Have no fear, Addgene is here to help!  General Design Figure 1: Two proteins...the FP back together (Figure 1). This method is generally known as Bimolecular Fluorescence Complementation...proteins in Caenorhabditis elegans. Goudeau et al. Genetics. 2021 Cynthia Kenyon mRuby4 mRuby4(1-10), pairs...

  20. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...various optogenetics plasmid tools, visit Addgene’s optogenetics guide. More optogenetics tools – light-activated...of new channelrhodopsins for use as optogenetics tools. These tools include a pair of channelrhodopsins...fusion proteins with a variety of genes across the genome. Although this tool was published in 2011, it was...effectively targets your gene/region of interest is a key step for CRISPR/Cas9 gene editing. Dr. Masahito...sequence or gene of interest into the pCoofy vectors, select the appropriate pair of vector and gene primers...vector and gene primer for SLIC cloning. Amplify the pCoofy vector and the sequence of gene of interest... can be used to generate random mutagenesis libraries or to insert heterologous genes, reporters, or other...
Showing: 61 - 80 of 102 results