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We narrowed to 144 results for: INV-E

Showing: 61 - 80 of 144 results

  1. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ... In the late nineties, this was developed into an E. coli system, with the noncanonical amino acid in ...expression of phosphoserine-including proteins in E. coli (Zhu et al., 2022). It uses the TAG codon for...components, meaning that the final protocol may involve a sensitive balance of trade-offs. This balance...

  2. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...carries CRISPR libraries that target mouse, human, fly, E. coli, and T. gondii genes.  2) What genetic modification... are identified using next-generation sequencing (E). Considerations and tips for successful screens... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways...a population of mutant cells to identify genes involved in a particular phenotype. CRISPR can be readily...specific classes of human genes. What are the steps involved in a CRISPR screen? Performing a forward genetic...

  3. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...On the 11th day of CRISPR, Addgene gave to me: Cas-E-le-ven! (Cas11)🎵 On the 10th day of CRISPR, Addgene... the 12th day of CRISPR, Addgene gave to me: Noninvasive assessment of gut function using transcriptional...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...request from — Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the...for now-common business practices, like accepting e-signatures and designing a website users could easily...hear it all the time, but Addgene is a totally invaluable resource. I really appreciate the work you do...

  5. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ... were used to express the necessary components in E. coli (Klompe et al., 2019). Recently, the lab introduced...temperatures. Data adapted from Vo et al., 2020. In E. coli, the new streamlined VchINT system was shown...programmability: depending on the transposases involved, they insert either randomly, or at fixed genomic... L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon system. (B) ... a streamlined expression approach (Fig. 2A), involving two-plasmid or single-plasmid systems (Vo et al...insertions are not currently possible, unlike methods involving homologous recombination. Additionally, these ...

  6. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L...678 . https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine...and it may also be aligned to plasmids in our inventory containing the suspected contaminating sequence...

  7. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

    Type
    Blog Post
    Published
    Nov. 29, 2017, 2 p.m.
    ...projects underway in the Ellis lab including yeast and E. coli engineering, microbial cooperation, and antibiotic...team also investigates bacteria and extremophiles for biotechnology and has been directly involved in supervising... through Open Access. Chapter 7/Outro: Tom’s Involvement with iGEM and Addgene  Many thanks to our guest... He returned to academic research in 2005 to investigate synthetic biology at Boston University, working...

  8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...screens." bioRxiv (2018): 356626. 6. Tanenbaum, Marvin E., et al. "A protein-tagging system for signal amplification...Resources Read about CRISPRi and CRISPRa from the NIH E-newsletter ...-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to...

  9. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...us your plasmid information in spreadsheet format, e-mail us at  [email protected] Special cases Please...can distribute your bacterial strains if they: are E. coli that can be used under BSL1 conditions were...allow you to produce large amounts of your insert in E. coli, then this is a Bacterial Expression vector....deposit, associate the plasmids with a Principal Investigator (PI), provide us with your shipping address ...

  10. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...to cancer.   References and resources Milling, L. E., Markson, S. C., Tjokrosurjo, Q., Derosia, N. M.,...pRDA_526 vector to knock out 30 genes putatively involved in CD8+ T cell responses to cancer. The screen...

  11. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ... UBMTA Chapter 4 - Developing Addgene as a modern e-commerce company and establishing credibility Chapter...Operating Officer. Prior to Addgene, Kenneth was an investment banking analyst at Credit Suisse’s Technology...

  12. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire E, Morin X, Livet J. Neuron. 2014...2.1 cleverly combines Cre-mediated deletion and inversion. The Brainbow-2.1 construct can express one of... or M-CFP.) The construct contains two tandem invertible segments, each with two fluorescent protein coding...color possibilities. The remaining cassette can invert as long as Cre remains expressed; once Cre is removed...recombination is required to prevent constant inversion of the construct. Sanes and Lichtman used pronuclear...

  13. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...Thornhill, S. I., Parsley, K. L., Staal, F. J., Gale, R. E., Linch, D. C., Bayford, J., Brown, L., Quaye, M.,...Leavitt, A. D., Robles, G., Alesandro, N., & Varmus, H. E. (1996). Human immunodeficiency virus type 1 integrase... IDLVs were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt...wish you the best of luck with any experiments involving IDLVs. And remember: You can never trust a lentivirus...of SCID-X1 patients. The Journal of Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/...

  14. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ....1007/978-1-0716-2918-5_5 Haery, L., Deverman, B. E., Matho, K. S., Cetin, A., Woodard, K., Cepko, C.,....2019.00093 McDade, J. R., Waxmonsky, N. C., Swanson, L. E., & Fan, M. (2016). Practical Considerations for Using...This process builds on our former approach and involves reading primary literature, soliciting information...

  15. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...Anfinan, N., Al-Wasiyah,  mohammad khalid, Huwait, E., Gari, M., & Al-Qahtani, M. (2018). Evaluation of..., Ma, M., Sahay, G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D....sense once you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted...

  16. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...Martin Chalfie expressed GFP in exogenous organisms (E. coli, and later C. elegans) for the first time. After...ring that glows with a faint green light. To investigate this green glimmer, Shimomura collected many,... in learning more about fluorescent proteins, I invite you to download our Fluorescent Protein eBook and...

  17. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... phosphorylated amino acid to the genetic code of E. coli (Park et al., Science 2011). The Rinehart lab... methodology in two differents types of bacteria (E. coli and Salmonella typhimurium) by integrating DNA...expression and purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor...complex constructs constrained only by the ability of E. coli to maintain the final plasmid after transformation... complex DNA constructs, particular for those involving recombination elements such as FRT or Lox sites...of sequences in bacteria are time-consuming and involve many steps. The Drew Endy and Keith Shearwin labs...molecules, allowing labs to precisely and non-invasively control neuronal signaling. For more information...

  18. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...library of E. coli tRNA adenosine deaminases (ecTadA), fused it with dCas9, and transformed it into E. coli... (Komor et al., 2016 and Nishida et al., 2016) invented CRISPR-base editors by tethering cytosine deaminase...

  19. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...University of Pennsylvania.     References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal deletions in human cells...efficiency of the method. One observation was an inverse relationship between deletion size and frequency...outcome to deletions following paired DSBs is inversions [3]. These rearranged segments may also be informative...nondeleted allele is likely to bear an indel or inversion mutation [3]. In the CRISPR era, the genomic landscape...Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical...

  20. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...recombination persists even in recombinase deficient E. coli and under conditions used to minimize recombination...Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L...reading frame is inverted in relation to the promoter, hence the name, double-floxed inverted open-reading...transgene is no longer able to flip back into the inverse orientation (See Plasmids 101: FLEx Vectors). While...opposite orientation (direction of arrows) the inverted transgene is reverted into the sense orientation...
Showing: 61 - 80 of 144 results