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We narrowed to 615 results for: LIS

Showing: 61 - 80 of 615 results

  1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...gene that kills the cells expressing it? The answer lies in special strains of E. coli that tolerate the ...take up your insert. But exactly how does ccdB accomplish this? Let’s start with a brief history of the...

  2. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...why is FLEx useful? The power of the FLEx system lies in the ability to conditionally turn off one gene...optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses for FLEx switches...

  3. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic... trials The CRISPR therapies don’t stop with the list above! There are many, many more in the earlier ...

  5. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ....201409074 Tran NH, Rahman MZ, He L, Xin L, Shan B, Li M (2016) Complete De Novo Assembly of Monoclonal ...tested for antigen binding. If starting from an established hybridoma line, the antibody can be converted...being the source of irreproducible data (Begley & Ellis, 2012; Baker, 2015). This contributes to an enormous...Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A ...276 . https://doi.org/10.1038/521274a Begley CG, Ellis LM (2012) Raise standards for preclinical cancer...

  6. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...expression and purification. Li J, et al. Science. 2017. PubMed PMID: 28082411 Listen to the podcast segment...increased ECT-DNA. Diving further into TZAP function, Li and colleagues observed endogenous TZAP localization...PubMed PMID: 18094608 Listen to the podcast Segement on the pPSU Plasmids B. subtilis knockout and knockdown...recently deposited 2 Bacillus subtilis libraries with Addgene – the B. subtilis Single Gene Deletion Library—Kanamycin...2016. PubMed PMID: 27238023 Listen to the podcast segment on the B. subtilis libraries Transcription factor...Cells) acrIIA1 Listeria monocytogenes  pRAU168  No  pJH372  No  acrIIA2 Listeria monocytogenes  pRAU171...   acrIIA3   Listeria monocytogenes  pCSW65 N/A (Toxic)   None N/A   acrIIA4 Listeria monocytogenes...

  7. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...hyperpolarization. PSAM ligands The advantage of PSAMs lies in their ability to be specifically and potently...19.2019Jendryka M, Palchaudhuri M, Ursu D, van der Veen B, Liss B, Kätzel D, Nissen W, Pekcec A (2019) Pharmacokinetic...

  8. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...Zuckerberg Biohub Manuel Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement Andy May... Crispresso, that adhere to the same principles listed here. It employs biological best practices. For... is generally advised, there is little detail published on how to do it best. Scientists are typically...

  9. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...range 6.0 (yellow) - 8.0 (red)). Blood pH normally lies around 7.35-7.4 so mammalian cells are sensitive...secretion of lactic acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split...

  10. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...Addgenies has helped me get a much better idea of what lies ahead. Thus the “Meet an Addgenie” Instagram interview...choice. I spoke with our Financial Operations Specialist, Lucy Hammer, about why she did not go. She told...don’t get your PhD you’re set up for failure. Listening to Kenkel’s story has helped me realize that is...Scientists are often under a lot of pressure to publish their research and are not so keen on sharing their...came from Lucy Hammer, our Financial Operations Specialist. She said, “I’ve found the most purpose at Addgene...

  12. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ... CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods. Researchers...epitope tagging). NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received...

  13. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...become clear that the power of this editing platform lies in the diversity of nucleases and their applications...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...

  14. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...13226-x Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Donohoue, P. D....

  15. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ...

  16. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...in single cells. Find iPAK4 plasmids here! Lin D., Li X., et al. (2023). Time-tagged ticker tapes for intracellular...whole-brain reference atlas efforts, including a list of validated antibodies, on their webpage: mAb3D...

  20. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...PubMed Central PMCID: PMC1462937. Dietzschold, B., Li, J., Faber, M., and Schnell, M. (2008). Concepts ...Sorensen, S.A., Slaughterbeck, C.R., Wakeman, W., Li, Y., Feng, D., Ho, A., Nicholas, E., Hirokawa, K....advantage of AAV vectors is that there are well-established protocols for their production and purification...AAV1, -2, -5, -7, -8 (Taymans et al., 2007), AAV1 (Hollis et al., 2008) AAV6 (Towne et al., 2010), AAV8, ...PMID: 19098898. PubMed Central PMCID: PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R...
Showing: 61 - 80 of 615 results