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We narrowed to 162 results for: Mammalian expression plasmids

Showing: 61 - 80 of 162 results

  1. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...EsCas13d. Find the Yan et al. plasmids here Find the Konermann et al. Plasmids here How do Cas13d enzymes...cleavage does not occur when Cas13d is expressed in a mammalian system. Since Cas13d is functionally similar...specific editor in mammalian cells Not all Cas13d proteins are functional in mammalian cells, but Konermann...S0092-8674(18)30207-1. PubMed PMID: 29551272 Plasmids from this paper are available at Addgene. Yan...S1097-2765(18)30173-4. PubMed PMID: 29551514 Plasmids from this paper are available at Addgene. Learn... Resources on Addgene.org: Find RNA Knockdown Plasmids Check out our CRISPR Guide Read our CRISPR History...

  2. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...effectively biotinylate AviTag expressing proteins in bacteria, yeast, insect and mammalian cells. Check out Kay...more on this below) it can be incubated or co-expressed with avidin or a structurally similar protein ...biotinylating proteins with BirA. You can find plasmids that contain both AviTag and BirA at Addgene! ...purification of tagged transcription factors in mammalian cells and transgenic mice.” PNAS 100.13 (2003)..., M., & Ting, A. Y. “Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin...

  3. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools... of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the mammalian transcriptional...CRISPRa depended on continued expression of dCas9 and gRNAs whose expression varies depending on transgene...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...demethylation Optogenetic repressors of gene expression in yeast Megan McClean’s lab generated an optogenetic...the nucleus where dCas9-Mxi1 can repress gene expression. In the dark, the NLS is inaccessible and the... Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...

  4. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...has been shown to work in plant, Drosophila and mammalian cells, and it may find wide use in biological ...photoconversion One concern with photoconverting GFP is the expression level necessary to observe a signal. Red signal...efficiently photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible with other... Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM,...Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein Which Fluorescent Protein Should I...

  5. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...synthetase Mammalian Cells pCMV-DnpK dinitrophenyl hapten Mammalian Cells pANAP AnapRS Mammalian Cells ...rewards could be substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific aminoacyl-tRNA...Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids to Fluorescent Protein Fusions ...with natural chain termination at stop codons, expression levels of these tagged proteins are often very...oocytes, mainly). Nevertheless, tRNA suppression plasmids are available from depositing labs that have mastered...aminoacyl-tRNA Synthetase Mammalian Cells pDULE-ABK pyrrolysyl-tRNA synthase for aliphatic diazirine ...diazirine amino acids E. coli and Mammalian Cells pEVOL-pAzF synthetase for p-azido-l-phenylalanine E. coli ...

  6. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...spatiotemporal expression of a gene of interest. This type of control over gene expression is possible in... (RNAi) by feeding the worm bacteria expressing specific plasmids (L4440) that contain your gene of interest...signaling in the entire adult brain, using a plasmid that expresses a genetically encoded calcium sensor, GCaMP6...interested in C. elegans plasmids and resources check out Addgene’s Worm Expression page. Looking for more... eBook Read our plasmids 101 blog series Resources on Addgene.org Browse our plasmid collection pages...musculus) Let’s start with the most preferred mammalian model organism of many researchers: the mouse ...Mus musculus). Mice have many advantages as a mammalian model organism for scientists as they have a relatively...

  7. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...special: Strong expression: A CMV promoter drives robust gene expression in mammalian cells. Flexible ...empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing tagged proteins, and stay...stay tuned for mammalian expression and AAV plasmid sets launching later this year!  Find Addgene's own...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ...) Fluorescence from axonal boutons of neurons expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered... synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A., Negrean, A., Chen, Y., Iyer...powerful tools in a scientist's toolkit is the empty plasmid backbone: the DNA vector that contains everything...

  8. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ... smaller size of the plasmids. The Zhang lab, on the other hand, uses a 1 plasmid, single infection system... thousands of targets at once. That’s what our plasmids allow.” Wang’s library is designed to target a...have previously been stably transfected for Cas9 expression. The Yusa lab has used their library to screen...possible to conduct systematic genetic screens in mammalian cells,” David Sabatini, a senior author of one...). Genome-Wide Recessive Genetic Screening in Mammalian Cells with a Lentiviral CRISPR-Guide RNA Library...

  9. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ...pBABE-puro for retroviral expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs...for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're...packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids from Didier ...influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids from Shinya... have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene ...our stride. We shipped our 100,000th plasmid. A variety of plasmids continued to be popular years after...with all the new plasmids. So far, we have distributed over 10,000 CRISPR/Cas9 plasmids to more than 3,...

  10. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...showed that it can cleave plasmid and genomic DNA in a variety of mammalian cell lines, with an efficiency...interest. In contrast, RNA guides must be expressed from a plasmid or in vitro transcribed, and changes in...for short. Addgene has already distributed this plasmid all over the world, and the question on everyone...were unable to show TtAgo-mediated cleavage in mammalian cells, likely due to high temperature requirements...homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and.... Epub 2016 May 2. PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan...24531762. PubMed Central PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, ...

  11. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9 (expresses S. pyogenes...looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and...Within target cells, Cas9 machinery is transiently expressed and binds to target DNA. In the final step of ...large enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle. The earliest...description of their experimental protocol. The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9...into therapeutically relevant, non-transformed mammalian cells. These viral vector systems also work equally... well in dividing and quiescent, post-mitotic mammalian cells. Applications of CRISPR delivery by adenoviral...

  12. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...of bacteria that can cause disease in humans. Expression of this system is tunable via a rhamnose-inducible...compared to a mixture of two base editor enzymes in mammalian cells. Read the paper in bioRxiv Find Target-...lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages like reduced...packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the tetraloop of the sgRNA scaffold...Nucleic Acids Research Find the gRNA and packaging plasmids   Cytosine deaminase for RNA editing Feng Zhang...

  13. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...Experiment Cas9 expression systems Addgene carries a variety of Cas9 containing plasmids that have been...delivery systems can be found here “CRISPR 101: Mammalian Expression Systems and Delivery Methods”. Validating...great choices. Check out our SpCas9 containing plasmids, optimized for a variety of species.   Specificity...modify the genome but still want to affect gene expression? dCas9 can be used as a platform to deliver various... transcription by blocking its initiation. In mammalian cells, robust repression requires targeting dCas9...frequently fused to Cas9 for this purpose. dCas9-KRAB plasmids for repressing target genes in a variety of species...activation domains to the same genetic locus via co-expression of an epitope tagged dCas9 and antibody-activator...

  14. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...) in a plasmid backbone. A separate plasmid contains the transposase gene (red) for expressing the transposase....12137. PubMed. Find the Plasmids at Addgene Kowarz Lab plasmids Izsvak Lab plasmids  ...). The original plasmid is empty following the removal of the transposon; the plasmid is then degraded...constitutive expression (pSBbi derivatives) and one set of vectors for inducible expression (pSBtet derivatives...transposase enzyme.2.) The transposase enzyme is expressed (red stars) and binds the inverted repeats (green...transposition; and 2) a transposon containing the gene-expression cassette that can translocate within the genome...many SB tranposase variants for hyperactivity in mammalian cells. Their efforts resulted in a new and improved...

  15. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles. ...al., 2024). In MagIC-cryo-EM, target proteins (expressed as a GFP fusion in this proof-of-concept study...be used for applications beyond cryo-EM.   Find plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi...disease phenotype in an animal model. Find PE-eVLP plasmids here! The lab’s other recent deposit builds on...over the wild-type enzyme. Find optimized PASSIGE plasmids here!     Figure 3: Schematic of the PASSIGE...recombinase integrates a sequence from a donor plasmid containing a complementary site into the genome...Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and...

  16. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...about other Protein Tags in our Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins with... in a scientist’s toolbox. As we’ve covered in Plasmids 101, tags can help you determine localization ...protein of interest, purify it, or determine its expression level without the need for a custom antibody....testing indicated that inntags IT5, IT6, and IT10 expressed well in cells and did not form undesirable aggregates... high correlation for protein localization in mammalian cells." Nature methods 10.4 (2013): 315-323. Pubmed... size or viability compared to untagged GFP overexpression, while the rest of the fusions had some negative...

  17. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...Cpf1 orthologs that display cleavage activity in mammalian cells. Like Cas9 nucleases, Cpf1 family members...Francisella Cpf1 (FnCpf1), they employed an E. coli plasmid depletion assay to discover FnCpf1’s PAM sequence...Cpf1 nucleases would exhibit in vivo activity in mammalian cells. From 16 diverse Cpf1 candidates, Zetsche...multiplexing approach using a single crRNA array to express up to 4 crRNAs. Zetsche et al. also suggest that...efficiency of two Cpf1 orthologs known to be active in mammalian cells, LbCpf1 and AsCpf1. In both reports, on ...system." Cell (2015). PubMed PMID: 26422227. Find plasmids from this publication at Addgene. 2. Zetsche,....1 (2016): 31-34. PubMed PMID: 27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik...

  18. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...cells with 5’-P-ssDNA guides and an NgAgo expression plasmid to edit a gene in vivo. Try NgAgo for Yourself...gRNA complexes or with guide RNA- and Cas9-expression plasmids. Cas9 guide RNA delivery does however rely...sequencing on select regions of all distributed plasmids as part of quality control, but does not perform...unlike an RNA guide, it cannot be produced from a plasmid in cells. Additionally, in vitro assembly of NgAgo... dangerous, non-physiological temperature for mammalian cells and one which diminishes the endonuclease...(1), NgAgo has received much attention and the plasmid has been requested by labs around the globe nearly...can indeed be optimized for genome editing in mammalian cells. However, the fact that most of the researchers...

  19. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...Knockdown the expression of Gene X using shRNA expressed from a plasmid   Figure 1: Expression Level of Gene... used for plasmid-based experiments. What are control plasmids? Generally, control plasmids help to ensure...as the expression level of Gene X in cells treated with Plasmid A is similar to the expression level of...follow an example of a plasmid-based experiment, delineate its requisite control plasmids, and discuss why ...proper control plasmids would address and answer these questions. Types of control plasmids Part of planning...efficiency. An internal control may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or...Figure 1, an internal control, such as the GFP-expressing Plasmid B, could demonstrate whether the cells were...

  20. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...Resources on the Addgene Blog: Plasmids 101: The Promoter Region Plasmids 101: Cre-lox Light-Switchable...gene, it therefore determines the timing of gene expression and largely defines the amount of recombinant...lac inducible system is found in many bacterial expression vectors. Negative inducible promoter pBad is ...Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter AlcR pGGA008...Temperature inducible promoters Temperature sensitive expression systems are typically less leaky than chemically...chemically induced promoters; they show near-zero expression at regular temperatures but can be induced by ... promoters, in which a gene of choice is only expressed following exposure to a brief heat shock. In the...
Showing: 61 - 80 of 162 results