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We narrowed to 287 results for: PREP

Showing: 61 - 80 of 287 results

  1. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...pMD2.G for producing our ready-to-use lentivirus preps. You can find our lentivirus production protocol...

  2. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ... cultures with the clones you pick and only mini-prep the positive ones. Regardless of which method you... a few positive clones, the last step is to mini-prep these clones and submit the plasmids for Sanger ...up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine template, primers...other colony PCR tips are highlighted below. Preparing template: Pick a single colony with a sterile ...

  3. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...In research there is a saying that if the sample prep is poor, then the data will be poor. The same is...assistants, and sitting out on the bench waiting for you, prep for research work has to be done by the researchers...highlight how to prepare and things to keep an eye out for during training. Preparation before bench work...the training. In this blog we will review the preparation and planning you can do to build a bench mentor-mentee... training in the lab! If you don’t do proper preparation and pay attention during training, then you won... you – so do your best to show up as your most prepared, can-do self and make the most of it! So, what... schedule, be respectful of theirs! Show up prepared and take training seriously, as reviewed above...

  4. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...eliminate any HEK-293T cells. If you titrate your viral preps, lentiviral titration requires an additional couple...Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols...transfection). This differs from typical insect cell preparations, which we talk about below. Processing The viral...

  5. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    Published
    July 28, 2020, 1:15 p.m.
    ...plasmid you didn't have to grow the bacteria and prep the plasmid before you begin your cloning experiment...Great news. A subset of our collection has been prepared as part of our new cloning grade DNA service and...plasmid without spending time and resources in preparative experiments.  We’re excited to begin offering...etc. and/or directly long term storage. Every preparation is quality controlled through next generation...

  6. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    Published
    Jan. 23, 2018, 2:04 p.m.
    ...experimental details on the spreadsheet: Name your viral prep Enter the specific names of the plasmids you plan...goes, “failing to plan is planning to fail.” Preparation is a key step to any experiment and can help ...

  7. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...started a new service distributing ready-to-use viral preps in 2016 and we just reached our 10,000th viral vector...

  8. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...submissions will receive a 20% off coupon for a viral prep. And if you've used the Data Hub to help select ...

  9. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...Lentivirus Get tips for titering your lentiviral preps Resources on Addgene.org: Check out the lentivirus...lentivirus guide Find ready-to-use lentiviral preps at Addgene ...expression using other methods.  In terms of preparation, VSV-G is stable and can withstand ultracentrifugation...

  10. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While those are the biggest...

  12. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...in other systems, binary vectors are cloned and prepped in E. coli. However, they also need to be able ...plant infection, as well as plasmid yield during preparation in E. coli. Addgene depositor Indu Maiti has ...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...cell populations. Find Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically engineered... Vectors for Your Research Get Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

  14. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...serotypes at Addgene. Serotype In-stock viral prep Packaged on Request AAV1  ✓ ✓ AAV2 ✓ ✓ AAV2...AAV Packaged On Request Browse all in-stock AAV preps Browse all AAV plasmids ...

  15. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ... is! Addgene has a number of ready-to-use viral preps available for a variety of broadly applicable and...

  17. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...Resources on Addgene.org Find Ready-to-Use AAV Preps Find Lab Protocols for Work with Virus Watch Our...Make sure your cells are as healthy as can be to prepare them for the arduous process of generating viral...

  19. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...adeno-associated virus (AAV). We also offer viral prep services for some of the plasmids in our collection...

  20. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...These sensors have been tested in vivo, in tissue preps, in multiple species, and in cell culture models...
Showing: 61 - 80 of 287 results