... cultures with the clones you pick and only mini-prep the positive ones. Regardless of which method you... a few positive clones, the last step is to mini-prep these clones and submit the plasmids for Sanger ...up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine template, primers...other colony PCR tips are highlighted below. Preparing template: Pick a single colony with a sterile ...

...eliminate any HEK-293T cells. If you titrate your viral preps, lentiviral titration requires an additional couple...Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols...transfection). This differs from typical insect cell preparations, which we talk about below. Processing The viral...

...In research there is a saying that if the sample prep is poor, then the data will be poor. The same is...assistants, and sitting out on the bench waiting for you, prep for research work has to be done by the researchers...highlight how to prepare and things to keep an eye out for during training. Preparation before bench work...the training. In this blog we will review the preparation and planning you can do to build a bench mentor-mentee... training in the lab! If you don’t do proper preparation and pay attention during training, then you won... you – so do your best to show up as your most prepared, can-do self and make the most of it! So, what... schedule, be respectful of theirs! Show up prepared and take training seriously, as reviewed above...

...experimental details on the spreadsheet: Name your viral prep Enter the specific names of the plasmids you plan...goes, “failing to plan is planning to fail.” Preparation is a key step to any experiment and can help ...

...plasmid you didn't have to grow the bacteria and prep the plasmid before you begin your cloning experiment...Great news. A subset of our collection has been prepared as part of our new cloning grade DNA service and...plasmid without spending time and resources in preparative experiments.  We’re excited to begin offering...etc. and/or directly long term storage. Every preparation is quality controlled through next generation...

...submissions will receive a 20% off coupon for a viral prep. And if you've used the Data Hub to help select ...

...started a new service distributing ready-to-use viral preps in 2016 and we just reached our 10,000th viral vector...

...Lentivirus Get tips for titering your lentiviral preps Resources on Addgene.org: Check out the lentivirus...lentivirus guide Find ready-to-use lentiviral preps at Addgene ...expression using other methods.  In terms of preparation, VSV-G is stable and can withstand ultracentrifugation...

...Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While those are the biggest...

...of a plasmid, the first step is always to send prepped plasmid DNA for next generation sequencing. We ...

...in other systems, binary vectors are cloned and prepped in E. coli. However, they also need to be able ...plant infection, as well as plasmid yield during preparation in E. coli. Addgene depositor Indu Maiti has ...

...cell populations. Find Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically engineered... Vectors for Your Research Get Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

... is! Addgene has a number of ready-to-use viral preps available for a variety of broadly applicable and...

...resource on Addgene.org Addgene's AVV guide AAV Viral Preps References  Earley, L. F., Conatser, L. M., Lue,...

...Resources on Addgene.org Find Ready-to-Use AAV Preps Find Lab Protocols for Work with Virus Watch Our...Make sure your cells are as healthy as can be to prepare them for the arduous process of generating viral...

...vivo imaging, PDGFR for bulk 1P imaging or in new preps. Using iGluSnFR3 in your lab The new iGluSnFR3 variants...

...adeno-associated virus (AAV). We also offer viral prep services for some of the plasmids in our collection...

...These sensors have been tested in vivo, in tissue preps, in multiple species, and in cell culture models...

...using lysed bacterial colonies, without the need to prep your plasmid. In this case, you’d be using colony...

... program coordinator.   6. Virtual interview prep Prepare for your virtual interview just as you would...that program of your dream.  “Chance favors the prepared mind” by Louis Pasteur These tips are a set of...of common sense approaches that will prepare you for the competitive landscape beyond college. They will...guarantee of success. Don’t luck into an internship, prepare the road ahead for success. Every student should...