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We narrowed to 479 results for: RET

Showing: 61 - 80 of 479 results

  1. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...life science literature is a litany of false interpretation in cell culture for this reason. Unreproducible...just a few publications which have since been retracted due to cell line contamination: Epithelial Mesenchymal...even decades after it is initially isolated. Regrettably this is not usually the case if you obtain a ...colleague. Time and money are wasted, papers are retracted and there is a loss of reputation that can impede...working vials have been used up. This ensures you can retain the maximum stocks and use of the original Cell...

  2. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...using HR was found to block the acquisition and retrieval of contextual fear memories [5].  In a modified...other regions of the brain may be involved in retrieval of remote contextual memories. This study is an...real-time inhibition), and behavioral (remote memory retrieval) specificity. Optogenetics can be used to more...Twitter @derekpsimonphd.    References 1. Stuber, Garret D., et al. "Excitatory transmission from the amygdala...2012): 159-172.5. Goshen I, et al. Dynamics of retrieval strategies for remote memories. Cell. 2011;147...

  3. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ... constant variables that enable the correct interpretation of the effect of the independent variable you...controlled for in order to eliminate any alternative interpretation of the results. Typically, plasmid-based experiments...non-targeting control is critical to the correct interpretation of the results because it provides an important...limitations and you should be cognizant of these when interpreting your results. The key to biological controls...multiple replicates, and ultimately getting interpretable results: Figure 4: Expression Level of Gene ...

  4. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...determined by the organized smooth endoplasmic reticulum (OSER) assay. A major advantage of mCyRFP1 is ...ability to be coexcited along with EGFP while retaining an emission spectrum that is easily separable ...makes mCyRFP1 a powerful tool for use as a donor in FRET (fluorescence resonance energy transfer) and FLIM...Lin and Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the...mammalian mCyRFP1 or CyRFP1 expression plasmids, FRET constructs, and CaMKII and RhoA sensors. Laviv,... of ~50% and confirmed that the fusion protein retained the PAM properties of the individual Cas9. Kim...

  5. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment Epithelium) AAV1, AAV2, AAV4, AAV5, AAV8...AAVs may move toward the cell bodies of neurons (retrograde transport) or towards the synapse (anterograde...serotypes 1, 8 and 9, not only do they undergo retrograde and anterograde transport, but they also have... specific cell populations. Find Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically...share conserved mechanisms for anterograde and retrograde axonal transport." Human gene therapy 25.8 (2014...

  6. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...assaying a secreted protein, first block the Golgi apparatus with Brefeldin A to inhibit secretion and then...expand monoclonal cultures.  Interpreting flow cytometry data To interpret flow cytometry data users will... Kim C, Boulanger CM (2012) Flow cytometry: retrospective, fundamentals and recent instrumentation. Cytotechnology...

  7. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    Published
    Nov. 27, 2019, 2:03 p.m.
    ...Combinatorial strategies can also be used for retrograde tracing: visualizing neurons that received information...region. In the example shown in Figure 1, injected retrograde AAVs at one location result in opsin expression...viral vector related blog posts Learn more about retrograde AAVs Resources on Addgene.org Read our AAV ...guide Learn more about our viral service Find retrograde AAVs at Addgene ...

  8. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...the blue "Save Verified Data and Return to Article" button to return to the table listing all of the plasmids...the full plasmid sequence can be assembled or theoretical — it does not have to be entirely verified by...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the NEB Stable...process for all of the plasmids in your deposit, return to the Add Plasmids page and click the blue "Submit...

  9. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...derived from the E. coli Ec48 retron RT and the S. pombe Tf1 retrotransposon RT, respectively. Their small...years after their initial publication, the Liu lab returned to the reverse transcriptase domain for optimization..., A., Gao, X. D., Raguram, A., Richter, M. F., Everette, K. A., Banskota, S., Tian, K., Tao, Y. A., Tolar...w Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V., An, M., ...

  10. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase variant...This technique is very similar to the widely used FRET, but it eliminates the need for an excitation light...exhibited 3-5 fold increased brightness over previous BRET probes, with sufficient signal for in vivo and in...Check Out All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein...

  11. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ... be fused to other proteins, and these fusions retained the ability to bind GFP. The idea of using GFP...T-DDOGs, GFP, and a reporter construct into the mouse retina. GFP expression robustly activated the reporter...Cre-DOG is both robust and specific. When tested in retinal electroporation studies, DsRed reporter expression...studies, and since cells infected with AAV-Cre-DOG retain normal neuronal function, this system may make ...

  12. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...Smith et al., 2024). They then used single-molecule FRET to study the intramolecular dynamics of the proteins...2: CLIP-SNAP fusion proteins for single-molecule FRET. A) Schematic of a CLIP-SNAP fusion protein (a.k.a...Garcia-Marcos’ lab recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors...ONE-GO biosensors measure the formation of Gα-GTP by BRET. The biosensor consists of a YFP-tagged G protein...

  13. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...cause an effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy...excites the fluorescence of another FP (e.g. YFP). FRET only occurs when the distance between the two FPs...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins interact...

  14. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...and stop. For instance (chr3, 1, 10) indicates a stretch of DNA at the very beginning of the third chromosome... Let’s now examine how we can locate powerful stretches of DNA known as enhancers with the help of genome...ESCs) with a lentiviral vector, an attenuated retrovirus in the same family as HIV. By design, our lentivirii...light on dark matter Precise knowledge of which stretches of the genome are pillars for stem cells, or metastasized...

  15. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...domain being tested. Find FRET plasmids here. Split EGFP: An alternative to FRET, split EGFP has also been... of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions between...concentrations and pH, using a range of strategies such as FRET, calmodulin, and others. Review Addgene's collection...

  16. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ... tips from experts on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers...Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied and used in assays ...Additional Resources on the Addgene Blog Tips for Using FRET in Your Experiments Learn more about GFP Controlling...

  17. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...Both PILATeS (18 kDa) and STReTCh (11 kDa) are significantly smaller than FRET-based mechanosensors (~60...be easily expressed and purified from E. coli, retains important functional properties, and is amenable...In this new variation on their previous sensor STReTCh, the 11-amino acid BiT fragment of luciferase is...

  18. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...evaluated set of values.  However, to evaluate the interpretation of the experiment would require access to each...no one is suggesting all data be stored in a retrievable format, we too rarely preserve “raw” data of ...journal repository, they are likely too large to be retrieved by others. Other new data initiatives are also...addition to enhancing veracity of collections and interpretation of data, publishing complete data, rather than...

  19. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...Evaluating Efficiencies of Dual AAV Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org...(2013) Effective delivery of large genes to the retina by dual AAV vectors. EMBO Mol Med 6:194–211 . https...truncated proteins are safe and effective in the retina of a mouse model of Stargardt disease. Hum Mol ...first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer...

  20. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...Filonov et al., 2013). FRET biosensors were developed based on miRFP760-miRFP720 FRET pair, including Rac1...indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were...optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol 14, 591-600 (2018). Shemetov, A.A...
Showing: 61 - 80 of 479 results