...and replaced it with a double-bonded oxygen. This simple enzymatic reaction enabled conversion between the...Researchers in the Xueli Zhang lab were making similar observations around the same time. (The papers ... journal on the same day.) This team created a similar construct that they called a Glycosylase Base Editor...(Huang et al., 2024). BE4max, a CBE, generated similar deletions at a lower frequency. Their data suggest...editing is still advancing rapidly. If it follows a similar path to base transition editing, we can expect ...

...adeno-associated viral vector (AAV) samples and created a simplified process, termed viral genome sequencing (VGS)...viral genome sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...routinely producing AAV. We believe that this simplified sequencing and analysis approach can be easily..., Leenay R, May AP, Paulk NK (2019) Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of ...

...the goal of encouraging user contributions (using simple web forms) while also maintaining a relatively ...register with a valid email address).  One may also simply attach important references that serve to further...more powerful ways, and the advanced search page simplifies the process of building advanced multi-parametric... few years with an emission peak around 600nm?  Simply input these parameters and you’ll find matching...

...overstated, we can also learn a lot from Nature’s simpler solutions to transmitting forces over long distances...engineer it to suit our purposes? In search of simple biological machines R bodies can repeatedly ...These questions can be addressed using R bodies: simple, force-generating protein machines that can extend...

...follow multiple fluorescently tagged species simultaneously. To do so with fluorescent protein labels requires...if you know that your protein of interest has a similar spectrum. For example, here's a screenshot from...Fluorescein / Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / ...of fluorescent proteins, imaging four colors simultaneously should be pretty straightforward. Thank You...

... next cloning experiment early on. Beyond these simple but powerful display improvements, when you click...for copy and paste functionality. Sequence Similar to the plasmid maps, the linear sequences hosted...hosted here are now interactive and have similar hover over features. As with the map tab, clicking on two...Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN...

...students focus on the application process, and simplify access to academic resources, we created STEAMid...worried about any eligibility provision, don’t guess. Simply reach out to the program coordinator with your ...through. The vast majority of programs tend to ask similar questions, thus devoting some time to writing a...Chemical Biology Program at Harvard. Roo wants to simplify access to academic resources among economically...

... thus contain complex internal cell structures similar to those of plants and animals. Unlike bacteria...mechanism of homologous recombination to facilitate simple gene replacement/mutation. Yeast expression plasmids...plasmid copy number within the bacterial cell. Similary, the specific ORI elements included within a yeast... Yeast Episomal plasmids (YEp): These are most similar to bacterial plasmids and are considered “high ...

...generating iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem...factors driven by one promoter. While lentivirus has similar rates of reprogramming when compared to MMLV-derived...advantages of this method are that it’s relatively simple to implement and doesn’t require time-consuming...advantage of using RNA to reprogram cells is that it’s simple and efficient. It has a high efficiency of reprogramming...also take less time to complete. Morphology is a simple and fast to evaluate. iPSCs should form compact...

...freezer.   RNA extraction methods evolved into a simple protocol still used today There are many alternative...for RNA extraction and purification. There is one simple method that works, and variations to that method...effectively separated from DNA and proteins by a simple extraction protocol with guanidinium thiocyanate-phenol-chloroform...View our protocol for RNA extraction! Making the simple protocol even more foolproof (still without a kit...

...described above can be used for similar applications and have similar advantages, though with some caveats...the lack of an Fc region. The lack of Fc also simplifies analyses when used in structural studies and ...Alternatively, Fab fragments can be used as a block in similar scenarios. By applying unconjugated Fab fragments...

...RNA. To protect the RNA from degradation, several simple methods have been employed which modify the sugar...make your gRNA more efficient on a budget is to simply swap out several of the ribonucleotides for deoxynucleotides...resulting in less off-target events. While both have similar advantages, bridged nucleic acids with N-methyl...RNA to control the entire editing system with a simple LED light. Lighting up your gRNA with fluorescent...

...CRISPR/Cas systems, however, greatly improved the simplicity of gene activation: rather than requiring protein...CRISPR activator. While it requires a relatively simple construct, it exhibits modest levels of gene activation...compared to the SAM system. Its activation levels are similar to that of SunTag. An advantage to this method ...Shi L, Cheng L, Huang P, Yang H (2018) In vivo simultaneous transcriptional activation of multiple genes...

...indirect ELISA is very similar to a direct ELISA, aside from the detection step. Similarly, the protein antigen...Boguszewska et al., 2019.   Competitive ELISA Similar to a sandwich ELISA, the competitive ELISA is also... visualization. The indirect ELISA is used for similar experiments as the direct ELISA, the only difference...

...organization scheme for multi-part DNA assembly. Simply put, it assembles individual DNA components into...begin with a promoter and end with a terminator. A simple TU could be comprised of just three parts: promoter...assembled into TUs is key to understanding MoClo. By a similar principle, TUs can be further complexed into multi-TU...Genetic circuits Most things in biology are not so simple, and engineering complex cellular behaviors requires...

...leading the process despite the recent advance. Similarly, grad students throughout universities that are...finding money in already tight lab budgets and even simply being able to categorize all postdocs at institutions...of institutional responses to the FLSA ruling. Similarly, from purely a salary perspective, while this ... advocate for the opportunities they present. Similarly, ongoing issues that affect all scientists; such...

...elements to turn off expression of one gene, while simultaneously turning on the expression of another! What...point mutation or functional mutation in vivo: Similar to a conditional rescue experiment with a wildtype...sense (on) orientation in the presence of Cre. A similar strategy can be employed for "Cre-off" (i.e. your...Boyden Bernardo Sabatini Wenbiao Chen Liquin Luo Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida...

...accuracy. Golden Gate assembly involves the simultaneous activities of a Type IIS restriction enzyme ...destination vector receives the assembly after similar Type IIS restriction enzyme cutting results in ...performed additional efficiency measurements using similarly sized Lambda amplicon inserts to control for any...assembly. The fidelity data can be applied to derive similar high-fidelity overhangs for any Golden Gate assembly...

...to show that they work well in plants and give similar stability to transcripts as the ones that people...we are collecting sequences that will provide a similar resource for those involved in monocot engineering...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...people to, for example, knock out a gene with simultaneous complementation, with a knockout and expression...

...getting-started guide for BLAST, which includes a simple explanation of the different BLAST programs, databases...results. The default setting is “Optimize for Highly similar sequences (megablast)”, which is very fast and ...often unreliable, we suggest using the “Somewhat similar sequences (blastn)” program for blastn so that ...options and for our suggested blastx customization. Similar to nucleotide sequences, proteins often have repeated...