We narrowed to 110 results for: actin

1. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ...means you aren’t getting any background or cross-reacting. We’ve developed this for transcription factors...

2. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   Published

   Aug. 7, 2014, 1:58 p.m.

   ...would like to try and meet. Don’t worry about contacting someone much more senior than you – meeting scientists...

3. RaPID Detection of RNA-protein Interactions

   Type

   Blog Post

   Published

   Aug. 16, 2018, 12:40 p.m.

   ...requires 16-18 hours to sufficiently biotinylate interacting proteins, which results in poor spatiotemporal...

4. Antibodies 101: Selecting the Right Antibody

   Type

   Blog Post

   Published

   March 15, 2022, 1:15 p.m.

   ...isolate an antibody’s immune complexes and confirm interacting partners by mass spectrometry (Uhlen, 2016). ...

5. Transferable Skills Guide: Problem Solving

   Type

   Blog Post

   Published

   Nov. 30, 2021, 2:15 p.m.

   ... description, but this skill is not just about reacting to technical issues. This skill also comes into...

6. Click Biology: How to Click and Play to Explore Biology

   Type

   Blog Post

   Published

   Oct. 21, 2025, 1:15 p.m.

   ...biology. Slow reactions are only good enough if the reacting parts are present at high concentrations, but ...

7. Transferable Skills Guide: Public Speaking

   Type

   Blog Post

   Published

   April 20, 2023, 4 p.m.

   ...doesn’t know the term “diving board” so you end up acting it out in front of a very confused group of visiting...

8. All in a Twist: dsRNA

   Type

   Blog Post

   Published

   Feb. 2, 2023, 2:15 p.m.

   ...handle most of these intermediates, with each one acting on specific substrates and in distinct cell cycles...

9. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

   Type

   Blog Post

   Published

   Feb. 2, 2018, 3:20 p.m.

   ...sucked to work remotely. I was also tired of the contracting life where you do a little bit of work for one...

10. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ...clean RNA, it’s important to make sure that when extracting, to only take the aqueous layer to avoid carryover...

11. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...active protein binding domain of Phytochrome B interacting factor 6 (PIF6APB), respectively. The cofactor...

12. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    Published

    Jan. 31, 2019, 1:32 p.m.

    ...tumor, proving once again that a tumor is always interacting with its surroundings and being shaped by them...

13. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    Published

    Oct. 22, 2019, 12:20 p.m.

    ...presenting. The latter is catchy, but beware. A distracting background or clashing colors can take away from...

14. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...behind from a preceding animal (ex: urine) can be distracting. If it is not cleaned, animals tested towards...

15. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ...permits it to bind to the CIB1 (cryptochrome-interacting basic-helix-loop-helix) protein. After fusing...

16. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...modifying other sites in the genome and directly interacting with transcription machinery.   Well, I’...

17. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...through structure-guided engineering of the PAM-interacting domain of Cas9. After multiple rounds of engineering...

18. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ...specific neuron firing are better equipped using fast-acting optogenetic tools. The advantages provided by chemogenetics...

19. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ...tend to be complex with a variety of proteins interacting. Experiments tend to be equally complex with ...

20. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ...more than once. Fibronectin is another membrane-interacting protein that can be used to enhance transduction...