We narrowed to 290 results for: cki

... be green, containing the desired mutation but lacking both RUBY and the construct that rescues the mutation...RUBY was integrated into a CRISPR system, then tracking the segregation of the CRISPR construct could ...

...transformation guru and instructor for our intro to biohacking courses. Our members pursue a diverse range of... molecules. Other members’ projects range from hacking probiotics to filming educational lab videos, while...

...centralized tracking when biological reagents are shared — both at what time and with whom. Tracking these ...

..., GPI, and SGZ, to improve neuronal membrane trafficking and localization. Improved photophysical properties...al…, Supplementary Figure 2.   Improved trafficking and localization through different C-terminal...

.... There’s also tips for setting writing goals, tracking progress, and starting a writing group. Loop Habit...literally a tomato! Image from Gerlos.   Tools for checking grammar and jargon Hemingway Editor  This is a...

...Accountability: At your next meeting, start by checking in on the action items from last meeting. Use ... Executive Director Joanne Kamens FInd Tips on Picking a Research Project Read Other Inside Addgene Posts...

...will define positive selection as the means of picking up positive traits (i.e. the plasmid contains a...toxin), whereas negative selection would be the picking up of a negative trait (i.e. the plasmid contains...

...— by directly typing terms in a search bar, by ticking some boxes, by selecting a suggestion — how should...should these be supported and combined?  Luckily, we had the advantage of a very specialized domain of ...

...than just picking up the phone and having a discussion. By only relying on email and never picking up the...

...information on this system! GPCR-APEX: Interested in tracking the downstream signaling pathways mediated by ... JA, Gygi SP, Kruse AC (2017) Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity...

...and so was really starting from the beginning. Luckily, a fellow graduate student and a data analyst in...comment in C++ and Java Command line history: Tracking command line history by recording all commands...

...HA staining is weaker than reporter staining. Picking the right reporter for your RANBody While all three... with these plasmids from Penn State Need help picking the right fluorescence microscopy technique to ...

...actionable way to tackle widespread irreproducibility. Luckily, there were other academics and like-minded organizations... post-workshop surveys, recording results, and tracking material downloads. This was particularly helpful...

...the features that would define a good tool for sticking together biological components. Nearly all of ...full advantage and have lots of fun building and clicking. References and Resources References Devaraj...

...Hegazy, et al., 2020; Niemeyer, et al., 2007; Stoeckius, et al., 2017). Metal isotopes are useful for ...–1930. https://doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister C, Stephenson W, et al (2017) Simultaneous...

...engineered mice: transgenic mice, knockin and knockout mice, and conditional knockin or knockout mice. While these...

...manipulate full genes and observe the effects. Luckily for us, CRISPR is an incredibly versatile system...et al. 2014).  Simple knock-out experiments are trickier when it comes to long non-coding RNAs (lncRNA)...

...right for your experiment. As always, we recommend checking your cloning via a miniprep and restriction digest...design. Note that you can browse all components by clicking the blue text in the upper right-hand corner.  ...

...Epigenetic engineering with single-pot assembly Tracking cell-cell interactions New auxin inducible degron...human body and G-baToN provides versibility in tracking and modulating the interactions between cells....

...another optimized platform. Gene Stacking As the name implies, gene stacking is the use of a combination of...