...$47,476 and the date for implementation was set for Dec 1 2016. In order to be exempted from the need to pay... these institutions (populations potentially from 1 to 5761, according to the NSF data) and therefore ...the time of writing responses to requests for the legal justification of this have not been received. Future...

...know that most cephalopods die after they lay their 1 clutch of eggs? Propagation is thus quite difficult...organism blog posts: Five Popular Model Organisms, Part 1 Five Popular Model Organisms, Part 2 ...next project. Gymnotiformes and Mormyridae (Jason Gallant) Weakly electric fish are excellent systems to ...

...vectors (e.g. pBV-Luc) and gene silencing (e.g. pLKO.1 - TRC cloning vector). Check out our complete guide...integration into the fly genome and are under the Gal4 inducible UAS promoter. Other vectors can be expressed...

...and drawbacks of four split AAV vector approaches. 1. Overlapping  Overview: The overlapping strategy has...Adeno-Associated Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org/10.1089/hgtb.2015.140 Frischmeyer...Expression from the hybrid vector, as measured by β-galactosidase activity, was 81% of that from a single vector...

...efficiently with adenovirus and AAV.   Table 1: Types of viruses and their features Choosing .... DOI: 10.1016/j.gendis.2017.04.001 Maetzig T, Galla M, Baum C, Schambach A. Gammaretroviral vectors:...

...accessible to practically everyone.     Fig. 1: Open source hardware builds   Since I started...components. In this field, often referred to as 'frugal science', existing components are adapted and combined...

...see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system... components: GBPa-VP16 (activation domain); GBPb-GAL4 (DNA-binding domain); and a UAS-driven luciferase...

...impacts of neural circuits on behavior. Figure 1: An overview of the chemogenetics system. Why use...Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson S (2019) Ultrapotent...

...editing machinery such as CRISPR-Cas. Figure 1: Schematic of targeted gene knock-out (left) and targeted...Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han Y-C, Ogrodowski P, Crippa A, Rekhtman N...

...fun together! Laura’s Top 10 Event Planning Tips! 1. Take suggestions Some of Addgene’s best events have...tournament or beer hour (only if it’s cool with your legal department) right in your breakroom. 5. Throw some...

... such a popular model organism? Zebrafish (Figure 1) were initially recognized as a useful vertebrate ... bacteriophage integrase phiC31. The popular UAS/Gal4 and Cre/Lox gene regulatory systems have also been...

...yourself in a creative rut, read on!   Fig 1: Blugene using creative problem-solving in the lab... weird face your fears and unleash creativity. Gallery Books. Boyes, A. (2019, November 13). 5 mental ...

...complex. So how exactly does this work? Figure 1: APOBEC- and Cas9-mediated editing (ACE) reporter ...D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris RS (2018) A fluorescent...

...indel formation and phenotypic changes as early as 1 week post-injection. Livers from these mice were histologically...Brötzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA (2019) CasX enzymes comprise a distinct...

...SpCas9 base editors with editing windows as small as 1-2 nucleotides! To reduce off-target effects associated...Lower on-target activity than BE3; editing window ~1-2 nt  87438  pET42b-HF-BE3  HF-Cas9 (NGG)  Very ...technique to beautifully demonstrate light-dependent Gal4 transcriptional activation and Cre recombination...

...mycoplasma can even escape 0.2-micron filters. Figure 1: Contamination! Tissue culture media often have indicators... mycoplasmas but also avoid other bacterial and fungal contamination. Proper PPE Since humans are the ...