...the expression level necessary to observe a signal. Red signal intensity increases with GFP concentration...For low-abundance GFP fusions, a photoconversion signal may not be observed. Sattarzadeh et al. found that...

... increased brightness, they also found that the signal to noise ratio can be increased from 2-fold (by.... Of these, 26 of the 46 loci gave a detectable signal in human U2OS cells. Based upon this, they chose...Hanhui et al. “CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.” Nature Methods...

...nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind cognate untranslated mRNAs....displacement leads to a change in the fluorescent signal: untranslated RNAs appear yellow, and translated...

...internal and external signals such as developmental cues and DNA damage. These signals activate a cascade... however have several limitations. First, FRET signals are often difficult to detect, and thus quantify...

... do it (i.e. where the signals come from and what implications their signaling is likely to have). Anterograde...neuron to another at synapses resulting in faint signals (Schwab et al., 1979; Buttry & Goshgarian, 2015...PubMed Central PMCID: PMC4678200.  Kohara, K., Pignatelli, M., Rivest, A.J., Jung, H.-Y., Kitamura, T.,...

...polyclonal, as their function is to amplify the signal from a primary antibody; the ability of polyclonal...their target protein thereby greatly increasing signal amplification.  When to stay away from polyclonal...positive control across experiments to be aware of signal strength. In general, polyclonal antibodies produce...

...one minute BASU generated a strong streptavidin signal (~250-fold enrichment) when compared to a scrambled...scrambled RNA motif control). BioID2 had no detectable signal at the same time point. E. coli’s BirA* was tested...timescale, such as during drug action or cell signaling. Limitations of RaPID Like other BirA*-based proximity...

...various labels to antibodies that produce detectable signals like light or color. In this post, we’ll go over...is not visible or capable of producing a visible signal, but it has a strong affinity for avidin and streptavidin... molecule. These properties allow for efficient signal amplification, making biotin conjugated antibodies...

...FRET signal. Subsequent demethylation separates the fluorescent units and so lowers the FRET signal back...a large role in turning genes ‘off’ and ‘on’, signaling which genes should be ‘read’ by the cellular machinery...

...neighboring sequence context. Sci Signal 13. https://doi.org/10.1126/scisignal.aax9730  Additional resources on...90746-4 Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell...

...is continuously detected via a fluorescent signal. The signal increases proportionally to the amount of...concentration of the starting template DNA the fluorescent signal throughout the reaction is compared to a standard...

...identification of signaling pathways with potential to confer anticancer drug resistance." Science signaling 7.357...Carlsbad, CA) that represents 180 genes from the Ras signaling pathway. Its major appeal is that it enables the...

...decreasing the fluorescent signal from the first FP and increasing the signal from the second FP. Several...Addgene has many of these constructs and they are designated mGFP, mVenus, etc. A word of caution is needed...

...immunodetection tools is their strong signal strength. This signal strength is due in large part to their... be labeled with up to 3 dyes, yielding similar signal strength to a standard IgG secondary antibody (...

...proteins, promoters, enhancers, polyadenylation signals, inducible gene-expression sequences, and more....promoter (to drive high-level expression) and a polyA signal (to terminate transcription) and the four constructs... containing multiple identical polyadenylation signals or promoters Gibson et al., 2009 MXS-Chaining...

...fluorophore-linked antibodies are used to visualize RNA. Low signal-to-noise ratios with such techniques can be problematic...fluorophore and subsequently leading to a positive signal. The same effect can be achieved for a fluorophore...than separate them (Fig. 1b). One can also make “signal-off” biosensors in which the aptamers fluoresce...

...found that adding a triple nuclear localization signal or including fusions with Histone 2A or 2B can ...biotinylation via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed by the desired nanobody...NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be exported to the periplasm...

... Fluorescent Protein Fusions Observing a bright signal for the first time after cloning an FP fusion with...illustrated the quantitative increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed... opposed to standard FPs. The increase in signal is not due to a brighter FP, but rather because of the...

...new improvements. The new variant has increased signal-to-noise ratio and better ability to record subthreshold...they are negative-going sensors (with fluorescent signal at baseline voltage), the investigators found that... SAS, splice acceptor site; pA, polyadenylation signal. Image reused from Riedmayr et al. 2023 under a...

...Microscopy-based lineage tracing can help illuminate how signaling pathways—such as those regulated by one-eyed pinhead—contribute... study also showed that in the absence of the signaling pathway regulated by one-eyed pinhead, incorrect...transform zygotes into adults and explain how signaling pathways control this process. Additional zebrafish...