...for Sensing and Signaling.... Plasmid Collections Synthetic Biology Sensing and Signaling Synthetic Biology...Biology: Sensing and Signaling SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...communications and environmental sensing. Sensing and Signaling Plasmids Search the table by keyword or sort by...

...assembled in collaboration with the Nuclear Receptor Signaling Atlas (NURSA)....

...antibody and conjugate chosen. Signal Amplification The amount of signal needed from a specific antibody...abundant target, it obscures fainter signals. Fluorophores can emit signals that overlap with each other. ... Other Affinity Reagents Handling Visualization Signal Amplification Application Overview Quantification...Fc region. This allows for the amplification of signals by having multiple secondary antibodies binding...sdAbs is usually achieved by conjugation of a signaling molecule directly to the sdAb, or by using a secondary... incorporated in the sdAb. sdAbs can have weak signals due to their monovalent nature. Darpins - Darpins...single arm of the Y). They can be conjugated to signaling molecules and are often used in clinical applications...

... retention signal moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention signal oxBFP Erik...structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI 98875 3xnls-mNeonGreen Nucleus...-derived (1-22 aa) import sequence and COX VIII signal peptide dL5 FAP; mCerulean3 Marcel Bruchez 44385...Lck-mTurquoise2 Plasma Membrane Lck N-terminal lipidation signal mTurquoise2 Dorus Gadella 98821 Lck-mScarlet-I ...Lck-mScarlet-I Plasma Membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella 50057 pLYS1-FLAG-MitoGFP-HA...49151 mito-BFP Mitochondria mitochondrial targeting signal (COX4) TagBFP Gia Voeltz 55102 mCherry-Mito-7 Mitochondria...pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP Thorben Dammeyer 54520 mCherry-Peroxisomes...

...sensing region of the construct leads to a change in signal of a circularly permuted fluorescent protein (cpFP...database includes many fluorescent biosensors that signal the presence of specific biomolecules, the activity...design, biosensors can enable investigation of a signaling pathway or measurement of a biomolecule while ...Conditions and constraints for astrocyte calcium signaling in the hippocampal mossy fiber pathway. Neuron... A polarized Ca(2+), diacylglycerol and STIM1 signalling system regulates directed cell migration. Nat...Single-color, ratiometric biosensors for detecting signaling activities in live cells. Elife. 2018 Jul 3;7....single-wavelength fluorescent sensor for cyclic AMP. Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. Justin...

...pro-proliferative and anti-apoptotic signals. mTORC2, which appears to signal upstream of Akt, also promotes...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen, energy ...levels, and growth factors. mTORC1 integrates these signals to determine the nutritional state of the cell ...cytoskeletal organization. Cancer Impact Enhanced signaling through either mTORC1 or mTORC2 correlates with...from David Sabatini & Kris Wood) References mTOR signaling in growth control and disease. Laplante M, Sabatini...

...of plasmids for cancer research, organized by signaling pathway. ... large variety of resources for use in cancer signaling studies, including mutant, knockdown, and overexpression...below organizes the plasmids and resources by signaling pathway, focusing on the main pathways involved.... These pathways are important to normal cell signaling and physiology, and, when dysregulated, can enable... mTOR mTOR is a protein kinase that anchors a signaling network for transitioning between anabolic and... Labs containing cDNAs for studying oncogenic signaling pathways. ClonTracer Library : Barcoding library...

... genes involved in innate and adaptive immune signaling and cell-cell communication....II molecules found on the surface of APC. BCR Signaling B cells mediate humoral immunity through the secretion... receptor (BCR). The strength and duration of signaling controls the fate of B cells and can lead to the...trigger. Interferons The interferon family of signaling proteins consists of Type I, Type II, and Type... 60 interleukins have been described. NK Cell Signaling Natural killer (NK) cells can kill cells that ...infected cell membrane, triggering cell death. TCR Signaling The T cell receptor (TCR) engages with antigen...Engagement of the TCR by an MHC molecule presenting its cognate antigen along with co-stimulation activates the...

...GTPases serve as molecular switches that transmit signals within the cell. GTPases have an active (GTP) and...physiological conditions GTPases play prominent roles in signaling pathways regulating cell migration, survival, ...growth, and differentiation. Schematic of Ras signaling cascade that starts at the plasma membrane. Cancer...containing SPRY SPRY1 SPRY2 SPRY3 SPRY4 Sprouty RTK signaling antagonist STK STK3 STK4 Serine/threonine kinase...;1773(8):1177-95. PubMed PMID: 17428555 . RAS signaling pathways, mutations and their role in colorectal....i5.97. PubMed PMID: 23799159 . Targeting RAS signalling pathways in cancer therapy. Downward J. Nat Rev...

...networks. Sensing and Signaling Browse plasmids related to intercellular signaling and environmental sensing...Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal...

... an aminoacyl-tRNA synthetase (aaRS) loads its cognate tRNA with a specific amino acid. Then, the tRNA...figure below: A plasmid expressing the tRNA and its cognate aminoacyl-tRNA-synthetase (aaRS) that has been ...typically the amber codon) that is recognized by the cognate charged tRNA. Once these plasmids have been introduced...plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin ncAA Expression...

...relationship between neuronal signaling and behavior. Depending on the signalling pathway the engineered receptor...small molecules, allowing a scientist to control signaling within cells expressing these engineered receptors...

... signal FNpcDNA3 - N-terminal FLAG and SV40 nuclear localization signal Myr Membrane...adds a N-terminal myristoylation signal For more information about tags, including amino...

...11086 Tac (lactose/IPTG inducible) MBP, 6x His, signal sequence for localization to the periplasm David...-terminal 6x His tag and an MBP tag. Contains a signal sequence to localize the protein of interest to...bacteria. The presence of arabinose enhances the FRET signal from the CFP and YFP fluorophores linked by an ...

... leading to the emission of photons. The robust signal, sensitivity, wide dynamic range, and resistance...substrate Akalumine with significantly brighter signal and emission in the near-infrared range, enabling... assess FKBP and FRP interactions in the mTOR signaling pathway in vivo . DULIP ( DU al L uminescence-...

...cells with unique epigenetic and gene expression signatures. Cell Res. 2011 Mar;21(3):518-29. Cheng piggyBac...cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012 May 29. Palecek...Chemical modulation of transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts...

...otif (PAM). The PAM sequence serves as a binding signal for Cas9, but the exact sequence depends on which...CRISPR-Sirius ), provide a better stability and enable a signal amplification for better imaging of genomic loci...in yeast. 2018. Casini A, Olivieri M, Petris G, Montagna C, Reginato G, Maule G, Lorenzin F, Prandi D, ...PMID: 30388409 CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.. 2018. Ma H, Tu...PMID: 26030444 2014 A protein-tagging system for signal amplification in gene expression and fluorescence...

...KGC-GFP-ER2 under the Syn promoter. Using bGHpA signal. Edward Boyden 115893-AAV8 pAAV-Syn-FLEX-rc [Archon1...floxed/reversed (Cre-dependent) manner. Using bGHpA signal. Edward Boyden 117382-AAV2 hSyn1-eYFP An AAV genome...for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 140135-AAV1 pAAV-EF1a-iCreV...

...protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/threonine-... an integration point for multiple biochemical signals. In collaboration with numerous research labs from...

...light to detect, measure, and control molecular signals, cells, or groups of cells with either actuators...genetically engineered cellular receptors that affect signalling pathways within cells. Subcellular Localization...