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Showing: 61 - 80 of 166 results

  1. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...of a tag or fusion with a reporter enzyme. For multicolor staining, careful consideration must be used ...staining with antibodies. Nanobodies also simplify multicolor staining experiments because they don’t require...

  2. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...In-vivo RGB marking and multicolour single-cell tracking in the adult brain. Gomez-Nicola D, Riecken K, Fehse...

  3. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...your experiment. Shorter amplicons tend to be better. Shorter amplicons make for shorter PCR programs...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ... 1), in China, Korea, Japan, Brazil, India, and Mexico. Table 2: Addgene distributors Distributor ...2015 Korea San Diego Biotech Lab 2018 Mexico e-nnovation Life Sciences 2020 India ...

  5. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...polypeptide chain. One part of the tRNA contains the anticodon,  a three nucleotide sequence complementary to... binds the amino acid that corresponds to the anticodon sequence. That means there are 61 possible tRNAs...

  6. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...base editing. They first created a defective chloramphenicol resistance gene (CamR) by introducing a point...): aaf8729. PubMed PMID: 27492474. 3. Gaudelli, Nicole M., et al. "Programmable base editing of A• T to...

  7. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...antibodies, making them difficult to use in a multicolor/multichannel imaging set-up. In contrast, smFP...specific reactivities making them more useful for multicolor imaging experiments than standard FPs. The Looger...

  8. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...sequencing data alongside the analysis of NGS-based amplicon sequencing data and found that the accuracy of...100-fold reduction in cost relative to NGS-based amplicon sequencing. To use the tool, you start by uploading...

  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...adeno-associated viral vector-mediated transduction of the corticospinal tract: Comparison of four promoters. Gene Therapy...Anterograde Transsynaptic Tagging: Mapping Corticocollicular Input-Defined Neural Pathways for Defense ...

  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ..., to produce a series of FP-RLuc8 fusions for multicolor imaging. In all of these fusions coelenterazine... of bright luminescent protein for real-time multicolour bioimaging. Nature Comm. 7, 13718. PubMed PMID...

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...causing dead cells to fluoresce.  When running a multicolor flow cytometry experiment, users must also prepare...Immunology 120: . https://doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le Van Kim C, Boulanger CM (2012) ...

  12. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...kits for plants that work for both monocots and dicots (Hahn et al., 2020; Chamness et al., 2023; Stuttmann... Multiplex Editing: One‐shot Generation of 8× Nicotiana Benthamiana and 12× Arabidopsis Mutants.” The ...

  14. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...experiment even begins! If you are performing multicolor experiments, you will want to be sure that the...Collection Find Photoactivatable FPs for Single and Multicolor in vivo Imaging Browse All Fluorescent Protein...

  15. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... a powerful tool to modify mammalian cells. Multicolor CRISPR labeling chromosomal loci in genome Thoru... School has recently developed a CRISPR-based multicolor tool enabling the imaging of multiple endogenous...2015. PubMed PMID: 26456817. Nano-lanterns for multicolor luminescence imaging  Fluorescent imaging techniques...

  16. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...Alexis Komor and the first adenine base editors from Nicole Gaudelli in David Liu’s lab in 2016. History of...deaminases. Adenine base editors The beginnings Nicole Gaudelli from David Liu’s lab created an adenine...

  17. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...transformation. Addgene depositors Sylvestre Marillonnet and Nicola Patron have assembled two collections of standardized... 10.1021/sb4001504. For further reading about Nicola Patron's MoClo kit and her plant synbio research...

  18. Antibodies 101 Series on TikTok

    Type
    Blog Post
    Published
    March 19, 2024, 1:15 p.m.
    ... #STEM #antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting ...

  19. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth day of ...

  20. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...deckthelab #virussnowflakes #sciart #thankyouscience #scicomm pic.twitter.com/DWKWaPs0iY— Xi Chen (@XiChen7889...
Showing: 61 - 80 of 166 results