...concentration with an Amicon column Apply 15 mL of PBS to the reservoir of a Amicon Ultra-4 30 kDa MWCO ...Columns, 7 kDa MWCO, 10 mL, Thermo Fisher 89893 Amicon Ultra-15 30 kDa MWCO column, Millipore Sigma UFC903024...2 with a buffer exchange/concentration using an Amicon 30 kDa MWCO buffer exchange column. (Optional) ...

...mL Bleocin 5 µg/mL Carbenicillin 100 µg/mL Chloramphenicol 25 µg/mL Coumermycin 25 µg/mL Gentamycin 10...

...5 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol 25 mg/mL (dissolve in EtOH) 25 µg/mL Coumermycin...

...Quantify the individual Maxipreps by Nanodrop, Picogreen, or Qubit. Each different DNA measurement type...