...resources on the Addgene blog Summer SciComm Series: Cognitive LoadSummer SciComm Series: Modes of CommunicationIntro...university I think you should check out.” A scicomm group project on the Leith river. Photo courtesy...CommunicationIntro to Our Summer SciComm Series!...

...Addgene blog Summer SciComm Series: Modes of Communication Intro to Our Summer SciComm Series!...

...Welcome to the last of our Summer SciComm Series! We hope you've enjoyed this small glimpse into the ...Cognitive Load SSCS: Preprints Intro to our Summer SciComm Series  ...

...Addgene blog:  Summer SciComm Series: Cognitive LoadIntro to our Summer SciComm Series    ...convince your audience to come to you (a TikTok scicomm account aimed at octogenarians is going to be a...contextualizing data. Often overlooked as a means of scicomm. Digital or print distribution.  Typically limited...people who are not already actively interested in scicomm. Difficult to do in-depth storytelling. Requires...

...second post in our Summer SciComm series and it’s time for one of my favorite scicomm bandstands: cognitive... permanent store of knowledge (a schema).”  In scicomm, I like to think of cognitive load as the amount...resources on the Addgene blog Intro to our Summer SciComm Series...

...Welcome to our Summer SciComm Series, where we’ll be talking about science communication, best practices...about my favorite thing - science communication (scicomm)! We have so many fun topics planned for this summer...question underlying all communications efforts.  Scicomm definitions (as used by Addgene)    Science communication...podcasts, science pub nights, and pop sci books. Most scicomm efforts fall under informal learning; however, ...

...gmail.com    For more info about starting your own SciComm organization and to learn about some of SITN's ...

... scicomm...

...have children but are interested in testing your SciComm skills or are interested in a career in education...

...Takeshi Imai from Kyushu University. Stochastic multicolor labeling is a powerful solution for discriminating...neuronal reconstruction. To achieve stochastic multicolor labeling, Brainbow used the Cre-loxP system to...introducing a mixture of XFP genes. Stochastic multicolor labeling is possible when each of the XFP genes...Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling of neuronal circuits with fluorescent...

...the teams at Addgene, ComSciCon, and many more great organizations. At ComSciCon, our primary mission ...with ComSciCon, find us on Twitter, Facebook, Instragram, or send an email to info@comscicon.org. Many...guest post was contributed by Nathan Sanders of ComSciCon, the Communicating Science Conference series for...positive asset throughout your career? 1. Make scicomm practice a priority The good news for young scientists...communicating the information you intended. 3. Join the scicomm community Like most aspects of science, communication...are passionate about communicating science.  ComSciCon's events have 3 main goals: To help graduate students...communication from across the US and Canada convene at ComSciCon's annual flagship workshop.  Our 2018 workshop ...

...on the Addgene blog Summer SciComm Series: Masters of SciCommSummer Scicomm Series: Modes of CommunicationCareers...Drummond & Fischhoff, 2017; Kahan et al., 2012). Scicomm in the community During my PhD, I’ve also found...

...will highlight the evolution of the  Brainbow multicolor labeling system - a perfect example of the continued...Nature. 2007 Nov 1;450(7166):56-62. PubMed. A technicolour approach to the connectome. Lichtman JW, Livet....1566. Epub 2011 Feb 6. PubMed. Flybow: genetic multicolor cell labeling for neural circuit analysis in ...

...in any dicot, but they were developed mostly with model plants in mind, Arabidopsis and Nicotiana benthamiana...Nicola Patron is Head of Synthetic Biology at the Sainsbury Laboratory, where she often feels more like...any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work in wheat...Twitter Addgene: You are active on twitter @nicolabiologist, where you tweet about plant synthetic biology...Tim-Martin Ehnert, Stefan Werner, Jonathan D. G. Jones, Nicola J. Patron, and Sylvestre Marillonnet. ACS Synth...

...Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This...persistently fluorescent during development. Stochastic multicolor labeling systems, many based on Brainbow, now...improving the level of detail possible for stochastic multicolor labeling of the brain. Labeling Drosophila neurons... of transgenic zebrafish lines that allow for multicolor labeling of an entire organism or a specific ...Zebrabow as a system that takes advantage of the multicolor labeling possible from Brainbow. Tracing clonal...researchers who developed Tetbow. Conclusion Stochastic multicolor cell labeling systems allow single cell dynamics...Central PMCID: PMC3713494. Ghigo, Clément, et al. "Multicolor fate mapping of Langerhans cell homeostasis."...

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... risk of an antibody.  In silico prediction of adverse reactions In silico assessment uses computer modeling... 2022.   Limitations of the in silico approach The in silico approach cannot replicate the complexity...from the in silico assessments, as well as analyze the contributing factors that in silico prediction ...responses vary depending on the person. However, in silico and in vitro assessment of immunogenicity can help... evaluate the immunogenicity of therapeutics in silico. These algorithms are trained on vast amounts of...and the efficacy.  Better together Combining in silico and in vitro approaches enables researchers to ...safer and more effective mAb therapeutics. The in silico method of immunogenicity assessment can help in...

...targeting the pMB1 replicon on pTargetF (Jiang et al., 2015). In addition to the pMB1 replicon, pTargetF contains...PrhaB, 2) changed the replicon of pCas into a non-temperature-sensitive replicon, and 3) added the sacB...for multicopy chromosomal integration (ex: for protein expression) (Zhang et al., 2020). Multicopy chromosomal... Most recently, we’ve developed a new system “Multicopy chromosomal integration using CRISPR-associated... the S. pyogenes Cas9, a temperature-sensitive replicon for self-curing, an arabinose inducible lambda-red... expression of the sgRNA that targets the pMB1 replicon, resulting in cutting of the plasmid. At this ... the gRNA on pCas that is specific to the pMB1 replicon of pTargetF may exhibit a more serious leaky expression...

...been uniquely challenging to apply in dicotyledonous plants, or dicots. But Jae-Yean Kim’s lab recently "... find in this post: Optimized prime editing in dicots Self-labeling tags for single-molecule biophysics...neurodegeneration research   Optimized prime editing in dicots By Emily P. Bentley Prime editing (PE), initially...delivered with an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  ...editing tools PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase...suggesting the improvements may be broadly applicable to dicot species. In addition, they generated offspring tomato... improve rapid and precise plant breeding. Find dicot prime editing plasmids here! Vu, T. V., Nguyen,...

...experiment is targeted amplicon sequencing. The information from targeted amplicon sequencing is highly ... genome for off-target sites irrespective of in silico prediction. These techniques differ in important...heterogenous populations, low sensitivity Targeted Amplicon Sequencing Provided 0.01% (variable) Sequence-...rate and the repair product sequence. Targeted amplicon sequencing doesn’t tell the whole story ...These strategies can be used in concert with in silico prediction to create a more comprehensive list ...unbiased detection isn’t as sensitive as targeted amplicon sequencing. For example, GUIDE-seq seems to have...contrasts with detection frequencies of 0.01% in amplicon sequencing (Hendel et al. 2015), a significant...