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We narrowed to 256 results for: idua

Showing: 61 - 80 of 256 results

  1. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ... of antibodies from 19 COVID-19 convalescent individuals (Tong et al., 2021). Another version of the spike...determine how effective sera from vaccinated individuals are at neutralizing the different SARS-CoV-2 ...

  2. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...Fidelity, it turns out, might depend more on the individual sequence(s) than on how well that sequence fits...potential for mismatches, so you can redesign individual pieces as needed to improve fidelity. NEBridge...

  3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...takes about one day, even for six plates (576 individual plasmids)! These libraries are then QC’d, pooled...the raw data from each sample in our pool and individually assembles the reads into a single FASTA sequence...

  4. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...develop new working relationships with these individuals who often had different styles from their predecessors...organization speaks in “one voice” whilst among individual members, many opinions may exist. I learnt to...

  5. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...managing is not “one size fits all”. Manage the individuals in your team: learn what motivates them (keep...benefit of the team. By properly managing the individuals in your team you will have a much better chance...

  6. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ... publication page, and finally on down to the individual plasmid page. The depositing lab page contains...it would cost to buy each plasmid in the kit individually. If I order the plasmid, how will it be sent...

  7. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...additional experiments investigating the role of individual genes or entire pathways in a particular disease...specific gene within the genome (see Fig. 2). Individual gRNAs are designed in silico using publicly available...

  8. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...of genome engineering via CRISPR.  Though the individual topics are not surprising, the real value here...book left them wanting to learn more. There are individual paragraphs that may have warranted their own ...

  9. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...IgG subclasses. Instead, you can pre-incubate individual primary antibodies with secondary nanobodies ... with three IgG1 antibodies. Antibodies were individually incubated with a secondary nanobody conjugated...

  10. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...viral cocktail are injected, by chance lots of individual cells will be transduced with multiple viral ...notice in figure 4b how difficult it is to trace individual cell’s axons, particularly the two green neurons...

  11. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...label subcellular structures in live cells. Four individual expression constructs were created, each containing...cloning. The modules (Figure 2) are contained in individual plasmids and are each flanked by the same multiple...

  12. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...diluted in a series ranging from 1:2 to 1:32. Each individual step of the series is consistent at 1:2. When...the %CV of the standard curve, one averages the individual %CVs for each point on the curve. ‌The smaller...

  13. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ... within ~250 nm of each other. For reference, individual presynaptic terminals, which are jam packed with...their relative positions, allowing you to resolve individual points with a conventional, diffraction-limited...

  14. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...run the journal club. Some are led by a single individual who walks through the paper figure by figure....implications rather than diving deep into the individual data points. All formats should be an open forum...

  15. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...successfully expressed as fusions that retain their individual activities (1). Researchers also discovered the...transcriptional activation (TA) domains retain their individual activities when separated (2), and that DBD and...

  16. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...enables users to conveniently select and deposit individual documents or more complex datasets into the desired... edit to particular groups and/or particular individuals, the issue of third-party access to the ELN arises...

  17. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...the years we have accumulated more than 300,000 individual nucleotide sequences. More than half of these...Quick-stepping to a FASTA file   With our outline of individual steps to automate plasmid sequence assembly, ...

  18. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...amplification. And as anyone who has worked with individual plasmids knows, some plasmids amplify well and... amplification, this step also depends on the individual library. So, on each library’s page, you can ...

  20. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...your modified pCAG-EGXXFP plasmid along with the individual gRNAs in their PX330 plasmids (or similar plasmids...being explored and it allowed control over each individual component of the system. However, it is easier...
Showing: 61 - 80 of 256 results