Viral Vectors 101: Producing Your rAAV
Type
Blog Post
Published
July 16, 2024, 1:15 p.m.
...or determination of the median tissue culture infective dose (TCID50) (Zen et al., 2004).
To choose the...production process.
Helper-free production
Unlike lentivirus, AAV does not possess the ability to replicate...are measured. A TCID50 measurement reports an infectious titer, meaning the concentration of viral particles...2010). It is recommended that you measure your infectious titer, since it will have more impact on your...Adeno-Associated Viral Vectors by Oxidant-Based Disinfectants. Human Gene Therapy, 32(13–14), Article 13–14...Bleu, T., Sommer, J. M., & Wright, J. F. (2004). Infectious titer assay for adeno-associated virus vectors... with sensitivity sufficient to detect single infectious events. Human Gene Therapy, 15(7), Article 7....