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Showing: 61 - 80 of 212 results

  1. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    Published
    Jan. 23, 2018, 2:04 p.m.
    ...each plasmid needed for the whole experiment Total volume (μL) of plasmid needed for the whole experiment... the equation is labeled with its corresponding column in the calculator spreadsheet: We hope that this...

  2. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...Red2Green, a program that uses this waste as plastic lumber for things like truck bumpers, plastic adirondack...this material to produce various grades of plastic lumber and distribute it to industrial and residential...

  3. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ... may be electrochemical or light-based, with luminescent and fluorescent reporters being especially popular...such as the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes. Examples ...

  4. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ... require injection of substrate to produce bioluminescence. Substrate pharmacokinetics and biodistribution...localization: In vivo imaging with fluorescent or bioluminescent reporters usually only detects the signal of...

  7. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...iGABASnFR, and accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses to ...electrical activity through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used...

  8. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ... The amount of aeration, temperature, culture volume, antibiotic, and medium can all affect copy number...conditions aren't working against you!  The culture inoculum Freshly streaked bacteria have higher copy numbers...

  11. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...assemble hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences...prepared as plexWell™ libraries, sequenced on an Illumina® sequencer, and then analyzed with our plasmid...

  12. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ... particles to your solution while still using a volume that’s compatible with your experimental setup.... the filter - When using a concentration filter column, make sure to rinse off the surface of the filter...

  13. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...new NEBNext Ultra II FS DNA Library Prep Kit for Illumina sequencing and took a class at NEB’s new teaching...prior NEBNext Ultra II DNA Library Prep Kit for Illumina, one was only able to extract libraries from previously...

  14. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...stored either in a dark, opaque vial or wrapped in aluminum foil to prevent ambient light from degrading them...

  15. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...calcium dynamics in the cytosol and sarcoplasmic reticulum are of interest to muscle biologists, while neuroscientists... Overview of AAV Encoded Calcium Biosensors Illuminating Epigenetics with a FRET Based Biosensor Fluorescent...

  16. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...direct the fluorescent protein to the Endoplasmic Reticulum (ER). If homodimerization occurs (most likely ...structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are visible. These whorls can be...

  18. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...either through a chromogenic or chemiluminescent reaction. Chemiluminescence, which is light produced by ...

  19. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...management software aren’t just limited to large volume facilities; it can be useful in academic labs where...collision. It may even be worth it to have extra columns in your database to the effect of `dev_name` and...

  20. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... expected range. For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than...state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased...
Showing: 61 - 80 of 212 results