...orchestrate the formation of high-functioning-but-temporary teams that have a specific purpose and finite ...year, or a few months, or even a few weeks. The important thing to remember is that every team should have...when I do come across one. Also, remember, one important feature that all projects have in common - and... What does a good team need? Besides that all-important sense of purpose, there are many more things necessary... function is certainly necessary, but equally important is making sure all team members understand what...their responsibilities are. That’s a subtle but important distinction. If I invite someone to be on a project...

...because it will be difficult or (more likely) impossible to find; therefore all the time spent collecting...t guarantee a publication. There is an art to composing manuscripts and the ASM Scientific Writing and...writer, I was unsure if I should possibly take a composition class in the English Department. He suggested... coming to the writing workshop in D.C. had to compose and submit a manuscript that they were working .... Practice writing everyday Overall, the most important instruction the classes taught me was to write...

...understand the specific components that make up a plasmid and why each is important. Our “Plasmids 101” series...molecular biology knowledge and techniques, and empower you with a firm understanding of the fundamentals...Drives transcription of the target gene. Vital component for expression vectors: determines which cell ...and selectively grown on antibiotic plates.  Importantly, because the bacteria from which plasmids are...

...from one man teams.  The composition of the management team is more important than the product itself ...CEO of miLab, a Boston-based startup focused on empowering early-stage scientists. You can think of miLab... that raising capital for such a fund is an impossibility at our early stage.  After each pitch we made...diverse educational backgrounds (an essential component to any successful startup management team). Sreyan...

...warfare [Vilensky & Redman, 2003]). Most importantly, these compounds cross cell membranes, thus enabling ...nonspecifically soak up your fluorophore package. One important concept in FRET experimental design is that the...donor first where labeling specificity is most important. Donor labeling and the need for specificity Donor...protein molecules contain the donor and, more importantly there is no fluorescence background resulting...contrast can be improved using disulfide-containing compounds such as British anti-Lewisite (which was originally...

...enough DNA and competent bacteria to ensure all components of the library are represented. Typically, Addgene...may be amplified preferentially, skewing the composition of the final recovered DNA. This bias can be ...need to count how many times each of the library components is found in the read list. If you also sequenced...give you a bell curve of the abundance of each component of your library. You should look for two things...hope this post has helped clarify some of the important considerations when using, amplifying, and verifying...

...biosensor with two components - an enzyme that breaks down the HMO of interest into its component simple sugars...particularly notes their ability to be precisely spatio-temporally controlled as a key advantage over other inducible...learn more about Xin’s work with photoswitchable components of the ERK signaling pathway in our recent blog...Oligosaccharides (HMOs). HMOs are thought to play an important role in nuturing the development of beneficial...

...responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation and ... fluorescence range to better study the spatio-temporal dynamics of apoptosis in living animals. To do...and (3) β-strand 11 (β11). Each part contains components and residues that are necessary for fluorescence...zebrafish, the Shu Lab used FlipGFP to track the spatiotemporal dynamics of apoptosis during embryo development...allow scientists to observe both the spatial and temporal dynamics of apoptosis both in vitro and in living...

...secondary importance (or tertiary, or non-existant). On the very first night of the iPSC symposium, a panel...might be able to attend the 10 Years of iPSCs Symposium earlier this year, I jumped at the chance. Here... most scientists recognize and appreciate the importance of ethics, the topic can often take the back ... the stem cell community for addressing these important questions that the scientific method cannot address...

...not E. coli. They fall under the heading of two-component systems (TCSs). TCSs consist of a histidine kinase...created the first E. coli light-sensitive two-component system (TCS). Levskaya et al. fused a photosensory...gene expression. An E. coli light-sensitive two-component system (TCS). A hybrid histidine kinase/photosensor...experimented with the promoter strength of various components, removing inducible promoters that could crosstalk...optimization of light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ....

...believe that communication is the single most important skill that scientists need to succeed in their... always recognized and valued for its immense importance, it may well be what determines whether you get...wasted if you cannot convince others that it is important and relevant to them.   When you think about it...you will also have to explain why the work is important and how it should change how the members of your...fields become excellent science communicators. To empower graduate students to become ambassadors for their...

...another way to activate gene expression, and two-component systems used in synthetic biology use light to...Addgene also has yellow flame light-regulated two component systems designed by the Tabor lab. Which inducible...promoter is right for me? Here’s a list of some important variables to consider when choosing an inducible...’re expressing a gene that may be toxic, it’s important that your inducible promoter not be too leaky....Region Plasmids 101: Cre-lox Light-Switchable Two-Component Systems for E. coli Browse other blog posts from...

...cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen ...the linearized vector by T4 digestion; then the components are combined and annealed. The resulting recombination...BioRender.com.   SLIC also opened up a new world of multicomponent assembly, as fragments with overlapping sequence...efficiency (~20%). Figure 2: Schematic of multicomponent assembly with SLIC. Multiple fragments are ...issue is sequence similarity. If fragments in a multicomponent assembly have 5′ or 3′ sequence homology to...

... You can do more than just ‘sense’ biological compounds and reactions; you can quantify them with biosensors... how to use viruses to set up these systems. Components of a biosensor Some of the biosensors used in... that you are interested in. The most obvious component of a biosensor is the thing that you care about...or an electrolyte such as calcium. This varied component, which we will refer to as the analyte, needs ... fluorescence, pH change, heat, etc. The last component of the biosensor is the signal transducer which...

...attended a scientific symposium called Horizons in Molecular Biology. This annual symposium is organized by ...designated work areas and come with health risks. Importantly, with this method it is not possible to follow...Chemistry, Göttingen in Germany. I attended this symposium seeking inspiration to resolve the technical hurtle...FUNCAT-PLA provides both quantitative and spatio-temporal information about the turnover of specific endogenous...

...any other important features that differentiate this plasmid from its predecessor. Important features ...restriction sites used during cloning, and most importantly, the predicted full plasmid sequence. Other information...sequence verify the plasmids. This is the most important step in ensuring quality, as it will catch any...sometimes have to design new primers to sequence the important features. You can use primer design software such...

...co-coregulator) partners. The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins...comprehensive datasets that collectively address many important and fundamental questions in nuclear receptor ...states, their circadian regulation, as well as in important physiological states such as adipogenesis and ...for a reasonable fee. We at NURSA recognize the importance of this service to the NR research community ...coregulators. We look forward to growing this important relationship with Addgene for the benefit of the...

...the following four components into 293T cells. Figure 1: The key components of Nanoblades. Image...CRISPR applications. Let’s take a look at the key components of Nanoblades, how their editing efficiency and...for HDR editing and mouse-model generation. Key components of Nanoblades Nanoblades are a Cas9 RNP delivery...transgenic mice by microinjecting zygotes with CRISPR components. However, this sometimes requires intracellular...

...length can and should be varied as it can have important implications for the fusion protein effectivity...included for visualization purposes, but the key component of the editing assemblies are sensor-actuators...upon these original tools that allows for both spatiotemporal protein degradation and quantification of this...expressed under a desired promoter with a specific spatiotemporal expression pattern and an auxin inducible degron...Resources page.  Plasmids for delivering CRISPRi components by a single AAV vector are now available from...

...specific tissues and cell types. If desired, spatiotemporal control of transgene expression or activity...effective in both mouse strains. It is therefore important to review the literature for use of a capsid in...Monkeys and rodents. Image and caption adapted from Campos et al., 2023. AAV.CAP-Mac is a systemic capsid...730825. https://doi.org/10.3389/fimmu.2021.730825 Campos LJ, Arokiaraj CM, Chuapoco MR, Chen X, Goeden N...Wang, T., Wolfe, D. A., Arokiaraj, C. M., Ngo, V., Campos, L. J., Griffiths, J. A., Ichiki, T., Mazmanian...Ravindra Kumar, S., Arokiaraj, C. M., Plattner, V. M., Campos, L. J., Mich, J. K., Monet, D., Ngo, V., Ding, ...Scherrer, J., Winchester, J., Blackburn, R. J., Campos, L. J., Man, K. N. M., Sun, J., Chen, X., Lefevre...