We narrowed to 233 results for: ogn

... to cleave 4 gRNAs separated by 28 base Csy4 recognition sites from a polycistronic transcript. Although...only a few elements of tRNAs are necessary for recognition and cleavage. This sequence flexibility indicates...

... tagged with a Spaghetti Monster because they recognize this GFP scaffold itself. Fortunately the GFP1...GFP1 HA Spaghetti Monster RANbody doesn’t recognize the scaffold and is useful for detecting GFP. Spaghetti...

...to its target. While most secondary antibodies recognize broad isotype classes, it is possible to get secondary...multiplexed assays, using secondary antibodies which recognize specific isotype subclasses of primary antibodies...

...that arise during transcription are typically recognized by RNase H which hydrolyzes the RNA portion, ...Y., Hur, S. Cellular origins of dsRNA, their recognition and consequences. Nat Rev Mol Cell Biol, 23: ...

... have to worry about and that is something to recognize! Distributing plasmids, viral service, and more...every day in every way. Scientists know this and recognize it all the time. We love this feedback and are...

...even increase off-target effects, respectively. Recognizing these issues, researchers from the Zhang lab ...Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease." Nature 513.7519 (2014...

...be aware that there are some particular styles recognized to work well. It is important that your readers... that your text is easily read. Similarly, be cognizant of audience members that are color-blind; avoiding...

...insertion of a recognition sequence into the genomic DNA that could then be recognized by a DNA-binding...

...until either the PAM site or part of the guide’s recognition sequence is destroyed via mutation. By introducing...introducing a mutation in either the PAM or the guide recognition sequence in the donor DNA, you can guarantee ...

...techniques are free of specific restriction enzyme recognition sites. Thus, chaining methods are not necessarily...1961, Swartz et al., 1962), SalI, XhoI and MluI recognition sites, which contain CpG dinucleotides in their...

...minigenome. The viral polymerase complex then recognizes the encapsidated minigenome as a template for...gene (e.g. firefly luciferase). The polymerase recognizes the gene start signal and initiates transcription...

...optional fluorescence of FPs with facile antibody recognition of epitope tags. Thus they have created antigenic...imaging set-up. In contrast, smFP tags can be recognized by distinct, fluorophore-labeled antibodies with...

...DNA sequence containing the 38-50 bp sequence recognized by the serine integrase, finding tens of thousands...reverse transcriptases, followed bylanding site recognition and integration of cargo via Cas9-directed integrases...

... to fill this gap by both helping researchers recognize their MANY transferable skills learned while working...in which the goal is to generate unique ideas. Recognize ideas can come any time. Meetings shouldn’t be...

...sequence specific to a virus or pathogen. Once Cas13 recognizes and binds to the programmed sequence, it can ... Cas9 (Zetsche et al., 2015 ). Cas12a however recognizes a different PAM site and generates 5’ and 3’ ...

...organism? Zebrafish (Figure 1) were initially recognized as a useful vertebrate model based on their straightforward...learning larvae, providing unprecedented views into cognitive processes like decision making (Kim et al, 2017...

...their nests so can be studied over time. They recognize individual faces (I guess of other wasps) so can... can be used to study aspects of cognition. Spiralians - Crepidula atrasolea (Jonathan Henry) This black...

...target DNA (Cpf1 requires only a crRNA for target recognition and cleavage). C2c2 (now referred to as Cas13a... or C base pair. In bacteria, once Cas13a has recognized and cleaved its target RNA sequence as specified...

...ori typically contains iterons that serve as recognition sites for replication initiation proteins. These...depends on whether the host has the components to recognize the ori and allow for replication. However, if...

...factors and chromatin modifiers. These proteins recognize and bind motifs within the enhancer sequence, ...Differential expression of transcription factors, which recognize and bind to different DNA motifs, results in a...