Skip to main content
Addgene
Showing: 61 - 80 of 225 results

  1. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ... have to worry about and that is something to recognize! Distributing plasmids, viral service, and more...every day in every way. Scientists know this and recognize it all the time. We love this feedback and are...

  2. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...to its target. While most secondary antibodies recognize broad isotype classes, it is possible to get secondary...multiplexed assays, using secondary antibodies which recognize specific isotype subclasses of primary antibodies...

  3. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...that arise during transcription are typically recognized by RNase H which hydrolyzes the RNA portion, ...Y., Hur, S. Cellular origins of dsRNA, their recognition and consequences. Nat Rev Mol Cell Biol, 23: ...

  4. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...  Sugar phosphate backbone modifications The recognition sequence for CRISPR-Cas9 targeting is led by ...cytoplasmic and nuclear nucleases, as they will be recognized as foreign RNA. To protect the RNA from degradation...

  6. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...be aware that there are some particular styles recognized to work well. It is important that your readers... that your text is easily read. Similarly, be cognizant of audience members that are color-blind; avoiding...

  7. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...techniques are free of specific restriction enzyme recognition sites. Thus, chaining methods are not necessarily...1961, Swartz et al., 1962), SalI, XhoI and MluI recognition sites, which contain CpG dinucleotides in their...

  9. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...until either the PAM site or part of the guide’s recognition sequence is destroyed via mutation. By introducing...introducing a mutation in either the PAM or the guide recognition sequence in the donor DNA, you can guarantee ...

  11. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...optional fluorescence of FPs with facile antibody recognition of epitope tags. Thus they have created antigenic...imaging set-up. In contrast, smFP tags can be recognized by distinct, fluorophore-labeled antibodies with...

  12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...target DNA (Cpf1 requires only a crRNA for target recognition and cleavage). C2c2 (now referred to as Cas13a... or C base pair. In bacteria, once Cas13a has recognized and cleaved its target RNA sequence as specified...

  15. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...the mutant versions of SpCas9 and SaCas9, which recognize PAM sequences other than NGG (Table-1). Type...or destruction of any restriction enzyme (RE) recognition site, RE analysis of PCR product could be used...

  16. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...sequence specific to a virus or pathogen. Once Cas13 recognizes and binds to the programmed sequence, it can ... Cas9 (Zetsche et al., 2015 ). Cas12a however recognizes a different PAM site and generates 5’ and 3’ ...

  17. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...from similar backgrounds, we would have similar cognitive biases and this would become apparent in the software... design. Each of us brings slightly different cognitive biases as a result of our diversity which in turn...

  18. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...ori typically contains iterons that serve as recognition sites for replication initiation proteins. These...depends on whether the host has the components to recognize the ori and allow for replication. However, if...

  19. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...DNA sequence containing the 38-50 bp sequence recognized by the serine integrase, finding tens of thousands...reverse transcriptases, followed bylanding site recognition and integration of cargo via Cas9-directed integrases...

  20. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ... to fill this gap by both helping researchers recognize their MANY transferable skills learned while working...in which the goal is to generate unique ideas. Recognize ideas can come any time. Meetings shouldn’t be...
Showing: 61 - 80 of 225 results