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We narrowed to 19 results for: ogn

Showing: 1 - 19 of 19 results
  1. Antibody Guide

    Type
    Collection
    ...capable of recognizing and binding to epitopes on antigens, they are also capable of recognizing and binding...source secondary recognizes], e.g. , “goat anti-rabbit.” Choose a secondary that recognizes your primary ...proteins, naturally produced by the immune system to recognize and bind foreign bodies. Antibodies can be used...proteins of interest. The protein that an antibody recognizes is called an antigen, while the specific binding... probability that an antibody will be able to recognize and bind to any given antigen. A mature B cell...specificity in antibody-based methods. Antibodies can recognize and bind other antibodies using epitopes present...important to choose a monoclonal antibody that will recognize an available epitope on your protein of interest...
  2. TALEN Guide

    Type
    Collection
    ... of a zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer... the ZF nuclease, the components of the array recognize individual nucleotides. For over ten years, zinc...identified a large number of zinc fingers that recognize various nucleotide triplets. With some trial and... and error, ZF arrays are able to recognize their targets with fairly high specificity. Fifteen years ...Iowa State University published the nucleotide recognition code of the TAL effectors, which were isolated...which single nucleotide the TAL effector will recognize: ( Figure 1B ) HD targets cytosine, NI targets...
  3. Genetic Code Expansion

    Type
    Collection
    ...interest with the modified codon that is recognized by the cognate orthogonal tRNA. Figure 1: Use of synthetase...have also been explored. A tRNA is engineered to recognize the reassigned codon. An aaRS enzyme is engineered...machinery. The orthogonal aaRS enzyme should not recognize endogenous tRNAs, and the orthogonal tRNA should...plasmid expressing the engineered tRNA and its cognate aaRS enzyme evolved to incorporate ncAAs. This ...plasmids also contain one or multiple copies of the cognate tRNA gene. ID Plasmid Synthetase Origin ncAA Expression...engineering RF2 and tRNATrp to mitigate native UGA recognition. Farren Isaacs Return to top Other Resources ...
  4. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...in cells or organisms. Each recombinase enzyme recognizes and binds pairs of short, specific DNA target...reporters . Experimental Considerations Recombinase Recognition Sites Each enzyme functions analogously but interacts...GAAGTTCCTATTCtctagaaaGTATAGGAACTTC Table 1. Summary of Cre, Dre, and Flp Recognition Sites. Controlling Recombinase Expression By ...interest and another FRT site. Flp recombinase recognizes and mediates recombination between the two FRT...RMCE): When matching but mutually incompatible recognition sites flank both a DNA target site and an exogenous...
  5. CRISPR Guide

    Type
    Collection
    ...regions, as it recognizes TTTV (V = A, C, or G) PAM sites. Engineered versions of Cas12a recognize different... mutations in multiple domains to expand PAM recognition, increase specificity, and decrease off-target...Faecalibaculum rodentium ) has increased fidelity, and recognizes NNTA, a PAM sequence often found in plants, expanding...mechanisms, including blocking crRNA loading or PAM recognition, inhibiting Cas9’s endonuclease domain, or inducing...EnCas12a, an engineered version of AsCas12a that recognizes TYCV and TATV PAM sites. The DETECTR platform...using Cas13 Cas13 is a type VI CRISPR enzyme that recognizes ssRNA. Type VI enzymes, which include Cas13a ...crRNA precursors to mature crRNAs. Upon ssRNA recognition by the crRNA, the target RNA is degraded. In ...
  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ... gfg, gfg-1 OGFR opioid growth factor receptor - OGN osteoglycin DKFZp586P2421, OG, OIF, SLRR3A OPRD1 ...triggers several processes to activate cells to recognize and get rid of infectious pathogens. The innate...immunity. Once an adaptive immune cell is primed to recognize a specific antigen, it finds and attacks pathogens...expand and give rise to memory cells that can recognize a recurrent infection and rapidly initiate a secondary...The cornerstone of the immune response is the recognition by T cells of foreign antigens on the surface...presenting cells. B cells in secondary lymphoid organs recognize specific antigens through the B cell receptor ...Engagement of the TCR by an MHC molecule presenting its cognate antigen along with co-stimulation activates the...
  7. Deisseroth INTRSECT Collection

    Type
    Collection
    ...placing two orthogonal recombinase (Cre and Flp) recognition sequences within synthetic introns. INTRSECT ...) of a molecular tool and adding recombinase recognition sequences (e.g. Lox sites, FRT sites) inside ...introns (A,D). The addition of these recombinase recognition sequences enables directional control of the ...
  8. Institute for Protein Innovation

    Type
    Collection
    ...antibodies exhibit distinct binding specificities — recognizing individual α or β subunits, or specific αβ subunit...developed recombinant versions of antibodies that recognize common epitope tags to help purify, detect, or...
  9. mTOR Pathway

    Type
    Collection
    ...key metabolic regulator, correlates with poor prognosis in many cancers.... either mTORC1 or mTORC2 correlates with poor prognosis in many cancers, including breast, colon, ovarian...
  10. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ... rather than DNA. In bacteria, once they have recognized and cleaved the target RNA sequence, they adopt...
  11. Antibody Production

    Type
    Collection
    ...in-house or through outside labs, to confirm recognition of the expected target or to confirm expected...
  12. Viral Vector Guides and Plasmids

    Type
    Collection
    ...necessary genes to achieve this goal. When a virus recognizes a host cell, it injects its viral genome into...
  13. Open Enzyme Collection

    Type
    Collection
    ...Eco31I 165507 pOpen-NotIR (GC) NotI (GC-rich recognition site) 165508 pOpen-HindIIIR HindIII 165526 pOpen-PstIR...
  14. Rett Syndrome

    Type
    Collection
    ...Age of diagnosis in Rett syndrome: patterns of recognition among diagnosticians and risk factors for late...
  15. Luciferase Plasmid Collection

    Type
    Collection
    ...EGFP-NanoLuc ( GpNLuc ) and LSSmOrange-NanoLuc ( OgNLuc ) fusions for BRET-based imaging and other applications...
Showing: 1 - 19 of 19 results