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  1. Antibody Guide

    Type
    Guide
    ...capable of recognizing and binding to epitopes on antigens, they are also capable of recognizing and binding...source secondary recognizes], e.g. , “goat anti-rabbit.” Choose a secondary that recognizes your primary ...proteins, naturally produced by the immune system to recognize and bind foreign bodies. Antibodies can be used...proteins of interest. The protein that an antibody recognizes is called an antigen, while the specific binding... probability that an antibody will be able to recognize and bind to any given antigen. A mature B cell...specificity in antibody-based methods. Antibodies can recognize and bind other antibodies using epitopes present...important to choose a monoclonal antibody that will recognize an available epitope on your protein of interest...
  2. CRISPR Guide

    Type
    Guide
    ...regions, as it recognizes TTTV (V = A, C, or G) PAM sites. Engineered versions of Cas12a recognize different... mutations in multiple domains to expand PAM recognition, increase specificity, and decrease off-target...Faecalibaculum rodentium ) has increased fidelity, and recognizes NNTA, a PAM sequence often found in plants, expanding...mechanisms, including blocking crRNA loading or PAM recognition, inhibiting Cas9’s endonuclease domain, or inducing...EnCas12a, an engineered version of AsCas12a that recognizes TYCV and TATV PAM sites. The DETECTR platform...using Cas13 Cas13 is a type VI CRISPR enzyme that recognizes ssRNA. Type VI enzymes, which include Cas13a ...crRNA precursors to mature crRNAs. Upon ssRNA recognition by the crRNA, the target RNA is degraded. In ...
  3. Promoters

    Type
    Guide
    ...requires an associated sigma factor for promoter recognition and binding. On the other hand, the process in...binding to the promoter region. Each sigma factor recognizes different core promoter sequences. Figure 2. ...eukaryotes. Operons consist of a promoter (which is recognized by the RNA polymerase), an operator (a segment...
  4. Molecular Cloning Techniques

    Type
    Guide
    ...distance away from the recognition sequence. Cutting distal to their recognition site allows for the creation...the insert and plasmid in such a way that all recognition sequences are removed from the final product,...
  5. Chemogenetics Guide

    Type
    Guide
    ...mutations that allowed them to respond to their cognate PSEM. For example, PSAM Q79G,Q139G is activated... (GlyR) chloride-selective IPD. Binding of the cognate PSEM allows for the influx of Cl - ions, and inhibits...sodium- or potassium-selective IPD. Binding of the cognate PSEM allows for influx of Na + and/or K + ions,...
  6. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... export. Psi (Ѱ) in cis RNA packaging signal; recognized by nucleocapsid proteins and essential for efficient...packaging. cPPT in cis Central polypurine tract; recognition site for proviral DNA synthesis. Increases transduction...
  7. Molecular Biology Reference

    Type
    Guide
    ..., and at least one unique restriction enzyme recognition site (Figure 2). These elements allow for the...-binding protein has some flexibility in the recognition sequence. Epitope tags, on the other hand, are...
  8. Lentiviral Vector Guide

    Type
    Guide
    ...protein binds. Psi (Ѱ) in cis RNA packaging signal; recognized by nucleocapsid proteins and essential for efficient...packaging. cPPT in cis Central polypurine tract; recognition site for proviral DNA synthesis. Increases transduction...
  9. Modular Cloning Guide

    Type
    Guide
    ...enzymes BsaI and BpiI/BbsI to cut outside their recognition site, leaving DNA fragments with an overhang ...
  10. Adenovirus Guide

    Type
    Guide
    ...infections, such as the common cold. The immune system recognizes adenoviral elements rapidly and can mount a strong...
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