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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 262 results for: rigi

Showing: 61 - 80 of 262 results

  1. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...applications. Get More Cpf1 Background Here THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN ... What we still don't know about Cpf1 In their original paper characterizing Cpf1, Zetsche et al. suggested...

  2. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...immune system of bacteria. These Acr proteins were originally found in type I CRISPR-Cas systems.   Four years...

  3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...Survival strain. For plasmids that contain the R6Kγ origin of replication, choose the Pir1 strain. If your...with scientists around the world. This post was originally written by Tyler Ford in March 2017 and updated...

  4. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...post. From Mice to Men When this article was originally written, the main focus was on editing animal... and targeted therapy possible. This post was originally written in 2014 by Kendall Morgan and updated...

  5. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...a fisherman would reel in a big catch. It was originally suspected that Cas3 moved a couple of kilobases...mutations if the chromosome is repaired with all the original DNA. With Cas3, you don’t have to play these odds...

  6. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ... Protein & Cell. Update (August 3rd, 2017) THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN .../ssDNA complex in the cell. As proposed in the original article, to achieve greater efficacy, researchers...

  7. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...Copy number is partially specified by a plasmid’s origin of replication, but can also be affected by the... lysate volume limits, the copy number of some origins of replication (e.g. p15A, ColE1) can be increased...

  8. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...modifications listed can refer to changes made to the original backbone by the depositing lab, or by a previous...previously deposited with Addgene, you can list the original vector’s plasmid number here. Gene/insert: This...

  10. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ... to community colleges, and has helped provide original research experiences for thousands of undergraduates...cytosolic MDH (hMDH1). They then expressed the original and recombinant enzymes in bacteria, purified ...

  11. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...at each step in the storage process. After the original sample is barcoded, the lab team takes over and...be transformed into different cells than were originally requested. Most of our samples (98.2%) transform...

  12. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...cross-contamination, we recommend retrieving plasmids from your original Addgene kit plate as few times as possible and...gets contaminated, you can resort back to your original Addgene kit plate for fresh bacteria. Performing...

  13. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... [IPI-SV5-Pk1] is a recombinant version of the original V5 tag mouse monoclonal antibody, which recognizes...4 protein, mScarlet3 was 50% brighter than the original worm-optimized wrmScarlet. mScarlet-I3, while ...

  15. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...citations so that people can go back and read the original reference for the data. For example, we summarized...how big the studies were, and you could find the original paper if you wanted to learn more. Another example...

  16. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...fold improvement in detection efficiency over the original CROPseq. Find CROPseq-multi plasmids here Walton...2021). Check out their paper for details on the original antibodies. Find Anti-SARS-CoV-2 Antibodies here...

  17. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...Addgene, you’ll usually receive an aliquot from the original library constructed by the depositing lab. (For... library should be very similar to that of the original library. Like amplification, this step also depends...

  18. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ... shown in Figure 2, this was likely due to the original transfection conditions. Now that we have successfully...PMCID: PMC3321166. Resources on the Addgene Blog Origin of Replication The Promoter Region – Let's Go! ...

  19. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...duplications (slightly larger bands sizes relative to the original template). Due to the small difference in size...mutated product can then be cloned back into the original plasmid using the unique restriction sites. Alternatively...

  20. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...phosphoserine-incorporation mutant for a residue that wasn’t originally a serine (e.g. mutating a threonine residue to... @nieminm.   References 1. Cohen, Philip. "The origins of protein phosphorylation." Nature cell biology4.5...
Showing: 61 - 80 of 262 results