We narrowed to 105 results for: transfection

1. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   Published

   March 24, 2016, 2:30 p.m.

   ... that are an order of magnitude greater than transfection approaches. Moreover, the cost of reagents is...

2. AAV Q&A with Tim Miles

   Type

   Blog Post

   Published

   May 17, 2022, 1:15 p.m.

   ...Animal Models Q9: Is there any difference in transfection efficiency when delivering these viruses via...

3. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ...IF-ICC.) This method was chosen because cells transfected with a plasmid to express the target antigen ...

4. Improving Transduction Efficiency with Exosome AAVs

   Type

   Blog Post

   Published

   March 14, 2019, 1:54 p.m.

   ...to modify it to improve yields. For example, transfecting the producer cell line with an expression vector...

5. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   Published

   Dec. 10, 2013, 3:13 p.m.

   ...cells after the plasmids have been introduced by transfection or electroporation. All you do to create a protein...

6. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

   Type

   Blog Post

   Published

   Nov. 6, 2018, 1:12 p.m.

   ...plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the plasmids used for these methods...Weintraub, and A.B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell,...

7. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ...an unregulated fashion and thus a marker for transfection. The other (mKate2) is regulated in the 3’ UTR...

8. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   Published

   March 19, 2019, 1:31 p.m.

   ...PPI) studies in plants that rely on transient transfection systems using Agrobacterium (such as Nicotiana...

9. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   Published

   June 1, 2017, 2:30 p.m.

   ...When a dish of mammalian cells is transiently transfected with a plasmid containing a fluorescent protein...

10. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...PubMed PMID: 26914317. 4. Mock, U. et al. mRNA transfection of a novel TAL effector nuclease (TALEN) facilitates...

11. Viral Vectors 101: Viruses as Biological Tools

    Type

    Blog Post

    Published

    March 21, 2023, 1:15 p.m.

    ...of cell types that are typically ‘difficult to transfect’, such as primary cells. They can also be directly...

12. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...base editor is split into two plasmids and co-transfected into cells for reconstitution. Image from Levy...

13. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...pSpCas9(BB)). The 2A-GFP allows for screening of transfected cells. Find pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR...

14. Working with Nuclear Receptors

    Type

    Blog Post

    Published

    July 15, 2014, 4:03 p.m.

    ...laboratories. The development of the transient transfection platform for assaying transcriptional activity...

15. 27 Hot Plasmids from 2016

    Type

    Blog Post

    Published

    Dec. 22, 2016, 3:03 p.m.

    ...single gene. The gRNA expressing cells were then transfected with a Cas9-expressing plasmid to generate a ...luciferase. The bait and prey plasmids are co-transfected and proteins are immunoprecipitated from the ... plasmid is also available). Second, they co-transfected short-arm donor vectors containing mAID and either...efficiently creating stable cell lines using direct transfection precluding the need for virus. Furthermore, ...

16. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...targeting experiments allows you to visualize transfection efficiency and even track localization in cellular...

17. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    Published

    April 14, 2022, 1:15 p.m.

    ...bonds, monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used...

18. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    Published

    Aug. 5, 2021, 12:15 p.m.

    ...hippocampal cultured neurons, imaged 14 days post-transfection, excited at 505 nm (right). Scalebar represents...

19. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ... plasmid). Viral production then proceeds by transfection of all three plasmids into a producer cell line...

20. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ... include both scrambled RNA motifs and cells transfected with BirA* protein alone (i.e. no RNA bait). ...